Organic material is widely used for propagation of orchids, namely coconut water, extracts of tomatoes, bananas, bean sprouts and potatoes which can be added to certain media as a provider of nutrients, amino acid minerals, and growth regulators. This study aims to determine the effect of the concentration of organic matter in coconut water and banana extract on MS media on the regeneration of PLB (protocorn like bodies) Dendrobiumgatton sunray. The study was conducted at the Biotechnology Laboratory, Faculty of Agriculture, Bengkulu University, from September to December 2019. This study used a Complete Randomized Block Design (RCBD) with two factors. The first factor was the concentration of coconut water with 3 levels namely: A0 = 0 (Control), A1 = 75 ml.L -1 , A2 = 150 ml.L -1 , and the second factor was the concentration of banana extract with 5 levels namely: B0 = 0 (Control), B1 = 75 g.L -1 , B2 = 150 g.L -1 . The results showed that coconut water did not affect all variables, however the banana extract had a significant effect on all variables except for the number of shoots. There was an interaction between the two treatments, namely the variable plantlet weight. Orthogonal polynomial analysis showed that there was an interaction between the two treatments, where coconut water and banana extract had a good response on the plantlet growth when given together as much as 75 ml.L -1 (A1) and 75 g.L -1 (B1). The concentration of both treatment was lower than it was given individually.
The black soybean (Glycine soya Sieb. & Zucc) is a species of legume native of East Asia, widely grown for its edible bean, which has numerous uses. Furthermore, it is used as a raw material in the soy sauce industry, and its protein and antioxidant content is preferred to yellow soybeans. This study was conducted to evaluate the in vitro callus growth pattern of four varieties of black soybean grown in various combinations of cytokinins and 2,4-dichlorophenoxyacetic acid (2,4-D), and was performed at the Agronomy Laboratory, Faculty of Agriculture, the University of Bengkulu. A completely factorial randomized design (RAL) with 10 replications was used in this experiment. The first factor was four varieties of black soybeans, namely Detam-1, 2, 3, and 4. Similarly, the second factor was the type of cytokinin, namely K0 = 0 (control / without plant growth regulators), K1 = 2 mg.L -1 BAP + 0.5 mg.L -1 2,4-D, and K2 = 2 mg.L -1 Kinetin + 0, 5 mg.L -1 2,4-D. The mature cotyledon explants were cultured on Murashige and Skoog (MS) Medium. The results showed that Detam 2, 3 and 4 black soybean varieties grown on MS + 2 mg.L -1 BAP + 0.5 mg. L -1 2,4-D medium produced more friable callus with clear green-yellow color as compared to those grown on MS + 2 mg.L -1 Kinetin + 0.5 mg.L -1 2,4-D. The greatest compact callus with yellow-green color were formed by Detam 1 variety planted on MS medium + 2 mg.L -1 Kinetin + 0.5 mg.L -1 2,4-D. The greatest embryogenic callus production (100%), the heaviest callus fresh weight (1.53 g) and the greatest fresh culture yield (1.58 g) of yellow-green callus with friable texture were produced by Detam 3 variety grown on MS + medium 2 mg.L -1 BAP + 0.5 mg.L -1 2,4-D.
Citrus hystrixis a famous horticutural crops product today. Fresh fruits of Citrus hystrix from Indonesia are mainly exported to European countries by PT Nusantara Segar Global under the trademark of Java Fresh. Futhermore, the seedlings of Citrus hystrix were also used to be a rootstock for RGL citrus due to its vigorous root growth, adataptive to marginal land, and resistance to dry season. The objective of research was to determine the effects of difference kind and concentration of cytokinin to induce shoot of Citrus hystrix. The research was conducted from January to April 2019 at the Laboratory of Biotechnology and Tissue Culture, Faculty of Agriculture, Bengkulu University. The research was designed to be Completely Randomized Block Design (CRBD). First factor was kind of cytokinin (BAP and Kinetin). Second factor was the concentration of cytokinins (0 ppm, 1 ppm, 2 ppm, 3 ppm, 4 ppm, and 5 ppm). Therefore, there were 12 combinations with 3 replications. The results show, the number of shoot leaf of Citrus hystrix shows a different respon to the type of cytokinin. The best type of cytokinin in the shoot of Citrus hystrix was BAP, that produced the highest number of leaves, the highest number of shoots and the heaviest wet weight of shoots, namely 10.42 leaves/bottle, 3.2 shoots and 0.46 gr. The optimum concentration of BAP was 2.58 ppm.
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