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Fusidic acid, an antibiotic produced from the Fusidium coccineum fungus, belongs to the class of steroids, but has no corticosteroid effects. It is indicated for the treatment of infections caused by methicillin-resistant Staphylococcus aureus strains. The aim of this study was to search for the properties of fusidic acid published so far in the literature, as well as the methods developed for its determination in biological samples and pharmaceutical formulations. From the findings, we can conclude that fusidic acid has been used for decades and is indicated for the treatment of serious infections caused by Gram-positive microorganisms to this day. Furthermore, it is a hypoallergenic agent, has low toxicity, shows low resistance, and has no cross-resistance with other clinically used antibiotics. The analytical method of high-performance liquid chromatography has been widely used for determining fusidic acid, since it can reduce the cost and time of analysis, making it more viable for routine quality control in the pharmaceutical industry.

Fusidic acid is an antibiotic steroid widely used for the treatment of serious infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Microbiological methods are indispensable to determine the mean percentage of antimicrobial in medicaments during manufacturing and quality control processes. The aim of this study was to develop and validate a microbiological method for the quantification of fusidic acid in dermatological cream by turbidimetry, using Staphylococcus epidermidis (ATCC 12228) and casoy broth as the culture medium. The validation parameters were in accordance with ICH specifications and demonstrated accuracy, precision, selectivity, and robustness, with linear ranges from 0.25 to 2.25μgmL(-1). This method is an alternative to the diffusion agar assay currently employed to quantify fusidic acid in dermatological cream, since it is sensitive, fast, and more economical.

Fusidic acid is an antibiotic steroid indicated for the treatment of infections caused by the genus Staphylococcus, including methicillin resistant Staphylococcus aureus strains, and other Gram-positive bacteria. In the present study, a stability-indicating reversed-phase liquid chromatography (RP-LC) method was developed and validated for the determination of fusidic acid in dermatological cream as an alternative to existing methods. Analyses were performed using a C 18 column and guard column at room temperature, eluting with an isocratic mobile phase of acetonitrile and water (72:28, v/v), adjusted to pH 3.5 with acetic acid, pumped at a flow rate of 1.0 mL min -1 , detection at 210 nm and 20 µL of injection volume. The forced degradation study was conducted under acidic, alkaline, neutral, photolytic, and oxidative stress conditions. The method was validated according to ICH and FDA guidelines; it was linear, precise, accurate, selective, and robust over concentrations of 5-95 µg mL , respectively. Therefore, we conclude that this method is suitable for quantifying fusidic acid in pharmaceutical dermatological creams and determining its stability, representing a more economical and practical alternative for routine analysis in quality control. Uniterms:Fusidic acid/stability-indicating/quality control/validation. Chromatography/reversed-phase.

The current study aimed to investigate if a pulp lesion, which is a local inflammatory disease, can promote alterations in insulin signal and insulin sensitivity (IS). Twenty‐eight two‐month‐old (230g) Wistar male rats were randomly divided into a control group (CN) and a pulp lesion group (PL). PL group was submitted to pulp lesion induction through the opening of the coronal pulp chamber of the upper right first molars by using a dental handpiece, followed by its exposure to oral cavity, promoting bacterial contamination and chronic inflammation. Thirty days after PL induction, the following evaluations were performed: 1) pp185 (IRS‐1/IRS‐2) tyrosine phosphorylation status (pp185 P‐Tyr), after insulin stimulation, in periepididimal white adipose tissue (WAT) and gastrocnemius muscle (G) by Western Blotting analysis; 2) IS determination by intravenous insulin tolerance test. The results showed that PL promoted: 1) a significant decrease (p<0.05) in the pp185 P‐Tyr in WAT (CN=152.9±12.7 vs PL=111.3±10.2 arbitrary units/ìg of protein, n=7) and in G (CN=133.1±9.1 vs PL=103.7±7.6 arbitrary units/ìg of protein, n=7); 2) a significant difference (p<0.05) in the IS (glucose disappearance speed rate: CN=3.04±0.26 vs PL=1.97±0.25 %min, n=7). These data suggest that PL is capable of changing insulin signal in both WAT and G, and causing insulin resistance in rats. Financial Support: FAPESP (2011/04255–8).