Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream

Abstract

Fusidic acid is an antibiotic steroid widely used for the treatment of serious infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Microbiological methods are indispensable to determine the mean percentage of antimicrobial in medicaments during manufacturing and quality control processes. The aim of this study was to develop and validate a microbiological method for the quantification of fusidic acid in dermatological cream by turbidimetry, using Staphylococcus epidermidis (ATCC 12228) and casoy broth as the culture medium. The validation parameters were in accordance with ICH specifications and demonstrated accuracy, precision, selectivity, and robustness, with linear ranges from 0.25 to 2.25μgmL(-1). This method is an alternative to the diffusion agar assay currently employed to quantify fusidic acid in dermatological cream, since it is sensitive, fast, and more economical.

Evolution of green chemistry and its multidimensional impacts: A review

“…In the literature there are many physical-chemical and microbiological methods for the evaluation of drugs and pharmaceuticals which contemplate green analytical chemistry items such as HPLC methods using only ethanol and water in the mobile phase (Tótoli and Salgado, 2014, Pedroso et al, 2016, Spagnol et al, 2016, Rodrigues and Salgado, 2016, Figueiredo et al, 2017, Kogawa et al, 2017, Marco and Salgado, 2017), spectrophotometry in the ultraviolet region (UV) using aqueous solution as diluent (Kogawa and Salgado, 2013, Alessio et al, 2017, Kogawa and Salgado, 2016), spectrophotometry in the visible region (Vis) using aqueous solution as diluent (Brbaklic et al, 2017, Rechelo et al, 2017), spectrophotometry in the infrared region (IR) using only potassium bromide as reagent (Moreno and Salgado, 2012, Tótoli and Salgado, 2012, Vieira et al, 2012, Kogawa and Salgado, 2013, Piantavini et al, 2014, Mallah et al, 2015, Marco and Salgado, 2016, Trindade and Salgado, 2017), capillary electrophoresis (CE) with migration time less than 5 min (Kogawa et al, 2014, Tótoli et al, 2015, Chierentin et al, 2016) and microbiological methods with results in 4 h (Kogawa et al, 2012, Tótoli and Salgado, 2013, Cazedey and Salgado, 2013, Vieira et al, 2014, Pedroso and Salgado, 2014, Chierentin and Salgado, 2015, Silva and Salgado, 2015, Tótoli and Salgado, 2015, Curbete and Salgado, 2016, Kogawa and Salgado, 2016).…”

“…A time-consuming method that releases results within 24 h or more, such as microbiological results for antibiotics, will make products expensive or, if released without this analysis, perhaps inefficient which can promote the overload of the health system and contribute to microbial resistance (Kogawa et al, 2012, Tótoli and Salgado, 2013, Cazedey and Salgado, 2013, Vieira et al, 2014, Pedroso and Salgado, 2014, Chierentin and Salgado, 2015, Silva and Salgado, 2015, Tótoli and Salgado, 2015, Curbete and Salgado, 2016, Kogawa and Salgado, 2016).…”

NIR‐Responsive Polyurethane Nanocomposites Based on PDA@FA Nanoparticles with Synergistic Antibacterial Effect

“…[40,41] The unique antibacterial mechanism of FA reduces the occurrence of cross-resistance with other antibiotics. [42,43] Hence, it is widely used in topical antimicrobial treatments of the skin. [44,45] Therefore, immobilization of FA on substrates for antibacterial properties due to the versatility and adhesiveness of the PDA NPs as multiple reaction sites.…”

Optimization of Microbiological Method by Turbidimetry for Quantification of Rifaximin Tablets: Validation, Application and Evaluation of Degraded Compounds

“…Rifaximin does not present microbiological method described neither in official compendia [11][12][13][14][15][16] nor in the literature and they are extremely important and necessary in the evaluation of power of antimicrobials [17][18][19][20][21][22][23][24]. The physical-chemical methods are unable to provide this type of information.…”