Seed storage under controlled temperature and humidity maintains the viability and the vigor of seeds for a long time while preserving their longevity. Ex situ conservation in a seed storage chamber is perfect for simulating the cool dry conditions that are associated with long-term seed storage. This helps to preserve the availability of seeds for the recovery of degraded areas and to ensure a supply of propagating material for various purposes in silvicultural production, or as raw materials for biotechnological use. The present work evaluated the physical and physiological quality of Enterolobium contortisiliquum (Vell.) Morong seeds from an ex situ collection that was kept in a seed storage chamber at 6 °C ± 3 °C and with 60% ± 5% of RH in waterproof packaging. The water content, the physical quality by X-ray tests, germination, electrical conductivity, and the integrity and quality of RNA that was extracted from the embryonic axes were all determined. It was possible to identify full, damaged, malformed, translucent, and empty seeds. The seeds that had been stored for 1, 5, 9, and 10 years presented 78, 82, 42, and 38% of normal seedlings, respectively, while the lots of 6 and 7 years presented 0 and 6%. The seed storage conditions were the same for all of the lots. A water content higher than 6% influenced the quality of the seeds. The E. contortisiliquum seeds demonstrated high longevity. They could be stored for up to 10 years under controlled conditions.
A fin de indagar la incidencia de los cambios acaecidos en los ámbitos urbano y rural, el objetivo del estudio fue caracterizar y comparar la expresión del crecimiento infanto-juvenil en los partidos bonaerenses de La Plata, Brandsen, Magdalena y Punta Indio. Se realizó un estudio antropométrico transversal en 7875 niños/as de 4,0-12,9 años de edad. Fueron relevados peso corporal, estaturas total y sentado, pliegues subcutáneos tricipital y subescapular y se calculó el Índice de Masa Corporal. La información socio-ambiental de residencia fue obtenida mediante una encuesta autoadministrada. Para estimar diferencias entre partidos fueron calculadas frecuencias y comparadas mediante pruebas de Chi2 y Tukey. Los datos antropométricos fueron analizados aplicando modelo lineal, considerando edad, sexo y localidad y sus interacciones. Los resultados indicaron que los niños de Punta Indio presentaron mayor tamaño corporal y ponderal junto con mayor reserva adiposa subcutánea, al tiempo que mayor porcentaje de padres y madres contaban con trabajo formal y niveles educativos más altos. Se concluye que, Brandsen, Punta Indio y Magdalena, circundantes al área metropolitana de La Plata, presentan sensibles diferencias socio-ambientales, aunque compartan semejanzas en el paisaje y tradición agropecuaria. Esa heterogeneidad se vería reflejada en la variabilidad del crecimiento de la población infantil.
Abiotic stress caused by rigorous environmental conditions, such as drought, has negative effects on seeds. The species from arid and semiarid areas have mechanisms to maintain seed viability. In this study, the analyses of physical and physiological quality of the seeds of Erythrina velutina and their response to drought stress and RNA extraction methods were carried out. The seeds were collected from mother trees in two different provenances of natural occurrences of the species. The physical quality, viability, and vigor of the seeds were evaluated by morphometric parameters, germination, and electrical conductivity tests. The seeds were submitted to drought stress conditions for 0, 24, 48, and 72 hours on -0.8 MPa polyethylene glycol solution. To evaluate the viable RNA extraction method, the seed embryos were used for the extraction of this molecule and with an assessment of quantity and quality. The seeds from Pernambuco that were harvested in 2008 were bigger and presented lower vigor when in comparison to seeds that were harvested from Sergipe in 2012. The seeds under drought stress conditions presented null germination for all the treatments. After the drought stress, they were germinated on a paper substrate moistened with water, with better responses from the Lot Sergipe, 2012. The imbibition of seeds in the solution for 24 hours provides an increase in germination for less vigorous seeds, being indicated as a pre-germinative treatment. The RNA quality obtained by using a commercial extraction kit has better results, providing materials without contaminants when compared to the TRIzol method.
Characterizing dispersal structures is crucial for species identification and selecting diverse germplasm. This study aimed to investigate the morphology of Spondias tuberosa dispersion units and assess the efficacy of x-ray imaging in characterizing their internal morphology. X-ray imaging successfully revealed the internal structures, enabling the identification of filled, translucent, malformed, and empty seeds. The morphological analysis provided valuable insights into the dispersal units and presented a non-destructive and efficient method for future germplasm studies.
Knowing the relationship between the physical and physiological characteristics of native forest seeds is essential for the successful recovery of riparian forests. Thus, the work was carried out to analyze the physical and physiological parameters of seeds of native forest species for use in the recovery of riparian forests by direct seeding. Were evaluated the physical quality (water content, mass, the weight of one thousand seeds, and the number of seeds per kilogram), morphometric characteristics (length, width, and thickness), and physiological quality in the laboratory (viability) of Cassia grandis L.f., Enterolobium contortisiliquum (Vell.) Morong., Guazuma ulmifolia Lam., and Libidibia ferrea var. leiostachya (Benth.) LPQueiroz. The experiment in the field was implanted in a randomized block design (RBD) with four blocks, testing seeds treated to overcome the four species dormancy. The direct sowing was in 10 planting lines, each composed of 10 pits (30 x 30 x 30 cm), spaced 2.0 x 1.0 m. E. contortisiliquum seeds, despite having the highest values for physical and morphometric characteristics, and having a high percentage of germination, was the species with the lowest percentage of emergence in the field, being below G. ulmifolia, which has seeds of smaller size and weight, and L. ferrea var. leiostachya which, despite having low germination, was the second with the highest percentage of emergence. The study allowed us to observe that there was no direct relationship between the physical and physiological attributes of forest seeds and their ability to emerge.
Areas undergoing forest restoration need to be monitored to achieve ecosystem services. This work was carried out to evaluate the establishment of the riparian forest area in the restoration process, after 18 years of intervention, through temporal analysis of the landscape, biotic and soil indicators. Moreover, a temporal analysis of the landscape, biomass and Gross Primary Production (GPP) was carried out, via geoprocessing, at five-intervals within the period from 2002 to 2020, as well as biotic indicators (seed rain, seed bank, seedlings), and edaphic indicators from 2019 to 2020. The seed rain presented 1,197 propagules, belonging to 27 different species. The soil seed bank showed higher density in the rainy season (21.3 seeds/m2), 25 seeds, seven species, and only one botanical family recorded. In the seedling bank, 1,193 seedlings were reported, belonging to 28 botanical families, in which 57% of the individuals are arboreal-shrubby of the forest. Edaphic conditions improved compared to the initial project implementation. In 2010 using the temporal analysis of the landscape, the planting area was 100% covered with dense vegetation. For biomass and the GPP is a linear increment over time. The successional stage of the area is advanced, with a significant representation of secondary and climax species. The monitoring favored understanding the dynamics of the restoration environment and conservation.
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