Seed Viability and RNA Extraction Methods of Erythrina velutina Under Drought Stress

Abstract

Abiotic stress caused by rigorous environmental conditions, such as drought, has negative effects on seeds. The species from arid and semiarid areas have mechanisms to maintain seed viability. In this study, the analyses of physical and physiological quality of the seeds of Erythrina velutina and their response to drought stress and RNA extraction methods were carried out. The seeds were collected from mother trees in two different provenances of natural occurrences of the species. The physical quality, viability, and vigor of the seeds were evaluated by morphometric parameters, germination, and electrical conductivity tests. The seeds were submitted to drought stress conditions for 0, 24, 48, and 72 hours on -0.8 MPa polyethylene glycol solution. To evaluate the viable RNA extraction method, the seed embryos were used for the extraction of this molecule and with an assessment of quantity and quality. The seeds from Pernambuco that were harvested in 2008 were bigger and presented lower vigor when in comparison to seeds that were harvested from Sergipe in 2012. The seeds under drought stress conditions presented null germination for all the treatments. After the drought stress, they were germinated on a paper substrate moistened with water, with better responses from the Lot Sergipe, 2012. The imbibition of seeds in the solution for 24 hours provides an increase in germination for less vigorous seeds, being indicated as a pre-germinative treatment. The RNA quality obtained by using a commercial extraction kit has better results, providing materials without contaminants when compared to the TRIzol method.

Bioindicadores florísticos do estado de conservação da mata ciliar do rio Pajeú, município de Serra Talhada – PE (Brasil)

“…Este fato está associado ao motivo dela possuir elevada capacidade de adaptação às mudanças climáticas do semiárido brasileiro, de modo que sua retirada influencia, negativamente, na qualidade ambiental da região. Souza et al (2020) enfatizam que o mulungu apresenta alta resistência à seca e rápido crescimento, tolerando variações de precipitações pluviométricas entre 326 e 2500 mm anuais e temperaturas de 22,4ºC a 26,6ºC. Sendo assim, considera-se esta espécie como euripotente, por suportar elevada variação hídrica nos períodos de escassez.…”