No abstract

HIGHLIGHTS The seeds obtained from the fruit pulp industry have a physiological quality for seedling propagation. There is a high potential for reducing fruit pulp industry residues by using these seeds for propagation. Higher pixel density is observed for full seeds with an efficiency of forming normal seedlings.

Storage of desiccation-tolerant seeds is one of the most effective germplasm conservation strategies; however, several species of tropical and subtropical humid forests have seeds that are sensitive to desiccation, and recalcitrant seeds, making conservation a challenge. Recalcitrant seeds deteriorate during storage due to high respiration rates and metabolic activity, and protocols employing osmoprotective solutions aim to minimize those effects to maintain seed viability for a more extended period. Hancornia speciosa, a fruit tree considered a priority for research in Brazil, is a desiccation-sensitive species. Thus, this study aimed to assess the physiological parameters of viability, vigor, and enzymatic activity of H. speciosa seeds stored in an osmoprotective solution. Germination percentage, water content, electrical conductivity, shoot, root, seedling length, peroxidase activity, and heat-resistant protein concentration were determined for seeds collected during summer and winter harvests. In addition, gene sequences were explored through gene ontology using Blast analysis to identify the biological and molecular processes associated with enzymatic action during storage. Summer-collected seeds performed better in viability and vigor and are recommended for storage in the osmoprotective solution. After being stored in the solution, seeds collected in the winter improved germination and vigor. H. speciosa seeds harvested in the summer or winter and stored in the osmoprotective solution remain viable for up to 90 days. Peroxidase and heat-resistant proteins are active; these enzymes' expression regulation should be investigated in future studies.

Abiotic stress caused by rigorous environmental conditions, such as drought, has negative effects on seeds. The species from arid and semiarid areas have mechanisms to maintain seed viability. In this study, the analyses of physical and physiological quality of the seeds of Erythrina velutina and their response to drought stress and RNA extraction methods were carried out. The seeds were collected from mother trees in two different provenances of natural occurrences of the species. The physical quality, viability, and vigor of the seeds were evaluated by morphometric parameters, germination, and electrical conductivity tests. The seeds were submitted to drought stress conditions for 0, 24, 48, and 72 hours on -0.8 MPa polyethylene glycol solution. To evaluate the viable RNA extraction method, the seed embryos were used for the extraction of this molecule and with an assessment of quantity and quality. The seeds from Pernambuco that were harvested in 2008 were bigger and presented lower vigor when in comparison to seeds that were harvested from Sergipe in 2012. The seeds under drought stress conditions presented null germination for all the treatments. After the drought stress, they were germinated on a paper substrate moistened with water, with better responses from the Lot Sergipe, 2012. The imbibition of seeds in the solution for 24 hours provides an increase in germination for less vigorous seeds, being indicated as a pre-germinative treatment. The RNA quality obtained by using a commercial extraction kit has better results, providing materials without contaminants when compared to the TRIzol method.

O conteúdo desta obra, inclusive sua revisão ortográfica e gramatical, bem como os dados apresentados, é de responsabilidade de seus participantes, detentores dos Direitos Autorais.Esta obra foi publicada pela Editora Itacaiúnas em maio de 2020.

Marker-assisted selection aims to improve quantitative traits in Eucalyptus, and molecular markers allow the identification of (the expression) of challenging alleles. Aiming to overcome this challenge our work presents a systematic review of the use of marker-assisted selection and candidate proteins to identify markers associated with traits of Eucalyptus. The research carried out a prospection of content obtained by keywords in the scientific databases Scopus and Web of Science. The bibliographic data generated was imported using the Bibliometrix package in R software. The nucleic acid sequences were prospected in the Genetic Sequence Database – GenBank. The proteins were prospected in UNIPROT and the 3D structures were obtained at the Swiss Bioinformatics Institute - SIB. The prospection resulted in 63 scientific articles after duplicates were removed, from 41 journals, with an average publication of 12.4 articles per year and an average of 25.2 citations per document. China and Brazil stand out in the number of publications. RAPD technique was the most used in studies to obtain alleles with traits of interest in Eucalyptus. The ISSR and SSR markers were highlighted for studies on genotype resistance. The sequences associated with resistance to galling organisms in Eucalyptus are related to TAC1 transcription regulatory proteins, mediator 2; zinc finger protein 11, associated with the expressed genes TAC1, At5g64680, and ZFP11.

<p dir="ltr"><span>A busca por inovações tecnológicas é uma importante ferramenta de auxílio para o planejamento da conservação das espécies florestais e seus produtos não madeireiros. Este trabalho objetiva realizar um estudo de prospecção científica e tecnológica do uso e do potencial de sementes florestais. Para a busca dos artigos, foram utilizadas as plataformas Scopus e Web of Science. As análises estatísticas foram geradas com o auxílio do pacote </span><span>Bibliometrix</span><span> do </span><span>software</span><span> R. As patentes foram prospectadas e analisadas usando-se o Orbit Intelligence. Foram mapeados 229 artigos e 387 documentos de patentes. A China é o principal detentor das patentes, incluindo produtos patenteados a partir de espécies florestais brasileiras. Embora o Brasil apareça como um dos países depositários, com destaque para as espécies nativas, ele não foi o país de origem de nenhuma patente da pesquisa. As patentes foram desenvolvidas principalmente para atender às indústrias de alimentos, de medicamentos e de cosméticos.</span></p>

Hancornia speciosa Gomes is a fruit and medicinal tree species native to South America, which in Brazil is considered of potential economic value and priority for research and development. We present a map of the state-of-art, including articles, patents, and molecular data of the species to identify perspectives for future research. The annual scientific production, intellectual, social, and conceptual structure were evaluated, along with the number of patent deposits, components of the plant used, countries of deposit, international classification and assignees, and the accessibility of available molecular data. Brazil has the most significant publications (306) between 1992 and 2020. Technological products (29) have been developed from different tissues of the plant. Most of the articles and patents were developed by researchers from public universities from different regions of Brazil. The molecular data are sequences of nucleotides (164) and proteins (236) of the chloroplast genome and are described to identify the species as DNA barcodes and proteins involved in photosynthesis. The compilation and report of scientific, technological, and molecular information in the present review allowed the identification of new perspectives of research to be developed based on the gaps in knowledge regarding the species and perspectives for the definition of future research. Supplementary Information The online version contains supplementary material available at 10.1007/s10722-021-01319-w.