The administration of high-fat diets, during 40 days to adult rats, provoked specific variations on serum and thymus fatty acids, as a consequence of differences in FA profile of their lipid sources. These results reflect the impact that eating habits have on health status. It is important to put emphasis not only on the reduction of total fat intake, but also on choosing healthy sources of fat, replacing saturated fatty acids by polyunsaturated and including oils with higher content of ω3 to keep a balanced ω6/ω3 ratio.
Objectives
Fatty acids have an important role in nutrition. The objective was to analyze the effect of diet containing olive oil, with and without the supplementation with omega 3, on serum, thymus and brain's fatty acid profiles of growing rats.
Methods
Weanling Wistar rats fed during 10 days a diet containing olive oil as fat (O group). Other group received the same diet supplemented with 24mg/day of fish oil (OS group). Control group(C) received diet according AIN´93. Serum, thymus and brain's fatty acids profiles were determined by gas chromatography. Statistical analysis used ANOVA.
Results
Results (%Area) were expressed as the Mean ± SD: SERUM: OLEIC O:23.44 ± 3.68a; OS:18.31 ± 2.22a; C:10.60 ± 2.01b; LINOLEIC(LA) O:12.44 ± 1.65b; OS:12.98 ± 4.31b; C:18.27 ± 2.81a;
LINOLENIC(ALA) O:0.30 ± 0.09b; OS:0.32 ± 0.08b; C:0.92 ± 0.34a; EPA O:0.65 ± 0.17b; OS:1.63 ± 0.49a; C:0.80 ± 0.23b; DHA: O:1.57 ± 0.58b; OS:4.00 ± 1.70a; C:1.33 ± 0.19b. THYMUS: OLEIC O:21.54 ± 5.92; OS:24.40 ± 5.04; C:18.22 ± 3.23; LINOLEIC O:5.90 ± 0.56b; OS:6.50 ± 0.61b; C:10.89 ± 2.18a; ALA O:0.27 ± 0.02b; OS:0.30 ± 0.07b; C:0.49 ± 0.19a; EPA O:0.49 ± 0.28; OS:0.50 ± 0.13; C:0.50 ± 0.12; DHA O:0.47 ± 0.10b; OS:0.70 ± 0.12a; C:0.52 ± 0.16b. BRAIN: OLEIC O:13.11 ± 2.64; OS:12.94 ± 1.07; C:13.14 ± 1.56; LA O:1.17 ± 0.46; OS:1.05 ± 0.33; C:1.26 ± 0.19; ALA O:0.15 ± 0.03; OS:0.12 ± 0.04; C:0.16 ± 0.06; EPA O:0.46 ± 0.18; OS:0.38 ± 0.09; C:0.33 ± 0.07; DHA: O:11.39 ± 2.04; OS:11.32 ± 1.69; C:11.66 ± 1.63. Means with one letter (a, b) in common, were not significantly different (p > 0.05). In sera, O and OS showed lower ALA and LA and higher oleic levels, compared to C. OS presented high levels of EPA and DHA. In thymus, O and OS groups showed lower levels of ALA and LA than C. DHA only increased in the OS group. No changes were presented in the brain.
Conclusions
The results suggest that olive oil exacerbated omega-9 family with diminution of essential fatty acids while organism tries to sustain brain essential fatty acids. Fish oil supplementation increased serum and thymus DHA levels, not modifying low levels of other essential fatty acids. Another source of supplementation may be convenient to analyze.
Funding Sources
Supported by University of Buenos Aires, Argentina.
The aim of this research was to evaluate the effect of tomato and tocopherols intake on IgE level in Lamina Propria of intestinal villi.An experimental rat model of protein malnutrition at weaning was used. Rats of Wistar strain were fed a protein‐free diet until they lost 25% of their initial body weight. This feeding procedure develops an allergic state. Re‐feeding was performed by the administration of an experimental diet containing 20% casein as the only source of protein (Re‐nourished group=R). Other experimental groups received this diet plus tomato (R+T) – equivalent to 200g/day for an adult man of 70kg‐ or plus a tocopherols mix (á, â, ã, ä) equivalent to those present in tomato fruit (R+Tph), added to drinking water during 35 days.Results showed that Lamina Propria of intestinal villi of R+T group presented higher % of IgE+ B cells compared to R (99±8; 62±11) and intake of tocopherols mixture reverted that situation lowering the levels to 31±6. These results would suggest that tomato could be an allergenic food as tested in this model. Instead, tocopherols were effective to alleviate the basal allergic status.
La anomalía de Ebstein (AE) consiste en el desplazamiento congénito de las valvas septal y posterior de la tricúspide hacia el ventrículo derecho. Gracias a esto, existe dilatación auricular por "atrialización" ventricular, insuficiencia tricuspídea y falla ventricular derecha. Un 30% se asocia con arritmias supraventriculares y síndrome de Wolff-Parkinson-White (WPW). Se presenta el caso de un paciente con ablación exitosa de vía anómala con coexistencia de AE y síndrome de WPW.
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