In this large, prospective, multinational cohort, more than one half of all cases of non-HACEK gram-negative bacillus endocarditis were associated with health care contact. Non-HACEK gram-negative bacillus endocarditis is not primarily a disease of injection drug users.
This work presents a new technique as a potential methodology to analyse seeds. The technology is known as dynamic speckle, or biospeckle, an optical phenomenon produced when active materials, such as biological tissue, are illuminated by laser light. In the present work, the biological activity of seed tissues has been inferred from quantitative and qualitative measurements of their speckle activity. The aim is to show that the biospeckle technique has a potential as a methodology to assess seed viability. One aspect that needs to be investigated is how the water content in the seeds affects bio speckle activity. An experiment has been performed to determine the effect of humidity in the results. Seed activity for different levels of humidity was determined using quantitative and qualitative methods. Also, in others experiments, viable and non viable seeds with different specific humidity levels could be classified using the same technique.
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Chemotaxis has a meaningful role in several fields, such as microbial physiology, medicine and biotechnology. We present a new application of dynamic laser speckle (or biospeckle) to detect different degrees of bacterial motility during chemotactic response experiments. Encouraging results showed different bacterial dynamic responses due to differences in the hardness of the support in the swarming plates. We compare this method to a conventional technique that uses white light. Both methods showed to be analogous and, in some cases, complementary. The results suggest that biospeckle processed images can be used as an alternative method to evaluate bacterial chemotactic response and can supply additional information about the bacterial motility in different areas of the swarm plate assay that might be useful for biological analysis.
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