Bio-speckle assessment of bruising in fruits

Pajuelo, M.; Baldwin-Olguin, Guillermo; Rabal, H.; Cap, Nelly; Arizaga, Ricardo; Trivi, Marcelo

doi:10.1016/s0143-8166(02)00063-5

Cited by 105 publications

(35 citation statements)

References 9 publications

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“…For example, studies carried out using this technique with bean seeds showed increased biological activity when they were inoculated with certain fungi, depicting an increased defense system against biotic stress (Rabelo et al, 2011). Apples that suffered mechanical injury showed a decline in activity at the location where the physical damage occurred (Pajuelo et al, 2003). According to Romero et al (2009), the biological activity of tomato fruits increased as they matured, while another study showed that a decrease in chlorophyll content was accompanied by increased biological activity in apple fruits (Zdunek and Herppich,2012).…”

Section: Discussionmentioning

confidence: 99%

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Rezende¹,

Soares²,

Santos³

et al. 2017

Aust J Crop Sci

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Cape gooseberry (Physalis peruviana L.) cannot tolerate high levels of salinity. Salt stress is one of the most damaging abiotic stresses that affects plant development. However, there are some evidences that silicon (Si) can act as a mitigating agent of environmental stresses. Towards understanding the stress using tissue culture, we investigated the effect of in vitro NaCl-induced salt stress in cape gooseberry and the potential mitigating effect provided by Si. Nodal segments were inoculated in MS medium with different combinations of NaCl concentrations (0.5 and 1.0% NaCl) and silicic acid (0.0, 0.5, and 1.0 g L -1 ). The experimental design was completely randomized, in a factorial design with a control treatment [(2x3) + 1], totalizing 7 treatments. The control treatment consisted of an additional treatment without the addition of any of the factors. Isoenzyme activity, proline content, CO 2 level and biological activity were assessed after 30 d. We found an increase in all antioxidant enzymes (SOD, CAT and POD) activities when salt stress was imposed, indicating that the plant has an efficient system to protect itself against stress. Among these enzymes, Si played a mitigating role only in POD activity. In relation to other evaluated enzymes, NaCl enhanced the EST and MDH activities, followed by a subsequent decrease in MDH. Si showed different performances according to the concentration of NaCl. Electrophoretic analysis represented by bands illustrated these behaviors. Proline content increased as salinity increased, and Si effect was observed by increasing the levels of this amino acid. High CO 2 level was found at the concentration of 1.0% NaCl, and Si contributed to decrease this variable to normal levels. The reduction in the respiratory rates may contribute to the allocation of carbon to other chemical reactions such as the synthesis of new tissues. Salt stress also increased the biological activity of leaves and there was no effect of Si for this variable. We conclude that salt stress causes a great damage to in vitro-grown cape gooseberry plants and the addition of 1.0 g L -1 Si can ameliorate that damage for some characteristics. However, more studies are necessary since the physiological changes played by Si have been poorly understood for dicots.

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Section: Discussionmentioning

confidence: 99%

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Rezende¹,

Soares²,

Santos³

et al. 2017

Aust J Crop Sci

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“…Speckle activity has been extensively studied and as results of several research projects in Optics a wide variety of parameters and methodologies for measuring the activity of such kind of speckle patterns have been proposed. In literature a wide range of applications are reported, from industrial applications like paint drying monitoring [1][2][3] to physiological activity of living objects such as blood flow, heartbeat, viability of seeds, maturity and bruising in fruits among others [4][5][6][7][8][9][10]. The metrological uses of dynamic speckle well consolidated by systematic testing of all the approaches arising by the many applications [11] suggest that it can be used as a tool for monitoring cultivation of microalgae in laboratory scale photobioreactor.…”

Section: Introductionmentioning

confidence: 99%

Bio-speckle for microalgae growth monitoring in laboratory scale photobioreactor

Romero¹,

Monaldi²,

Domínguez³

et al. 2017

Opt. Pura Apl.

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ABSTRACT:Microalgae are widely used in different industries -pharmaceutical, cosmetic, in those dealing with pollution prevention, aquaculture and especially biofuels. Because of the wide range of use, there is an active interest in microalgae large-scale cultivation. Standard techniques for algae biomass production requires cultivation in nutritious culture media, and well-defined external parameters, such as controlled conditions of light intensity, temperature and aeration gas. Commercial production of microalgae is expensive, and the addition of nutrients in culture media contributes to its high cost. The evaluation of the quality of culture media using wastewater effluents or agro-industrial waste can be a way to reduce algal cultures costs. This research aims to develop a noninvasive method for the testing of effectiveness of agro-industrial waste, previously treated, as culture media through daily monitoring of population increase microalgae. We found that it is possible to detect the growth of the microorganisms by using statistical cumulants as numerical approaches for analyzing the dynamic speckle patterns produced by samples of Chrorella Vulgaris algae cultivated in bold basal culture medium.Key words: Biospleckle, microalgae growing, speckle activity, autocorrelation function, optical density. RESUMEN:Las microalgas, son ampliamente utilizadas en las industrias farmacéutica, cosmética, en aquellas relacionadas con la prevención de contaminación, acuacultura y especialmente en biocombustibles. Debido a su amplio rango de aplicaciones existe un gran interés en el cultivo de microalgas a gran escala. Las técnicas estándar para la producción de biomasa algal requieren de su cultivo en medios nutritivos y parámetros externos bien definidos tales como condiciones controladas de iluminación, temperatura y aireación. La producción comercial de microalgas es costosa y la adición de nutrientes a los medios de cultivos contribuye a aumentar el costo. La evaluación de la calidad de medios de cultivos usando aguas residuales o residuos agroindustriales puede resultar en una vía para reducir costos. Este trabajo tiene por objetivo el desarrollo de un método no invasivo para monitorear la eficiencia de residuos agroindustriales, previamente tratados, como posibles medios de cultivos a través del monitoreo diario del crecimiento poblacional de las microalgas. Hemos encontrado que es posible detectar el crecimiento de los microorganismos usando cumulantes estadísticos como una aproximación numérica para analizar la actividad de patrones de speckle dinámico producidos por muestras de algas Chrorella Vulgaris cultivadas en medio basal Bold.Palabras clave: Biospleckle, crecimiento de microalgas, actividad de speckle, función de autocorrelación, densidad óptica. REFERENCES AND LINKS / REFERENCIAS Y ENLACES[1] H. Rabal, M. Trivi, R. Arizaga, N. Cap, G.Romero, E. Alanís, "Transient phenomena analysis using dynamics speckle patterns," Opt. Eng. 38(1), 57-62 (1996).https://doi.org/10.1117/1.600789[2] G. Romero, E. Alanís, H...

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Section: Introductionmentioning

confidence: 99%

“…Illumination of a test object with a speckle pattern is essential in the monitoring of biological activity [2], measurements of object roughness [3], thickness and range [4], and in microscopy [5]. All of these applications [2]- [5] use a fully-developed speckle field (FDSF). A speckle field is considered fully developed when the mean roughness height of the diffuser is in the order or larger than the light source wavelength λ and the illuminated spot size is much larger than the any lateral scale of the diffuser [6].…”

Section: Introductionmentioning

confidence: 99%

Object wave reconstruction by speckle illumination and phase retrieval

Almoro¹,

Hanson²

2009

JEOS:RP

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An innovative setup for the speckle-based phase retrieval method is proposed. In the conventional setup, a plane wave illuminates the test object and the transmitted wavefront is incident on a diffuser aperture generating a speckle field. The sampled speckle intensities at axially displaced planes are input into a phase retrieval algorithm based on a wave propagation equation. In the new setup, the arrangement of the diffuser and the object is reversed. A plane wave incident on the diffuser generates a speckle field which, in turn, is used to illuminate the object. The transmitted wavefront is then directed to the camera sensor. The advantage of the proposed setup is the increased resolution since the limiting aperture is the full area of the sensor.

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Bio-speckle assessment of bruising in fruits

Cited by 105 publications

References 9 publications

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Bio-speckle for microalgae growth monitoring in laboratory scale photobioreactor

Object wave reconstruction by speckle illumination and phase retrieval

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