Detection of Fungi in Beans by the Laser Biospeckle Technique

Braga, Roberto A.; Rabelo, Giovanni Francisco; Granato, Luis R.; Santos, Ederson F.; Machado, José C.; Arizaga, Ricardo; Rabal, H.; Trivi, Marcelo

doi:10.1016/j.biosystemseng.2005.05.006

Cited by 96 publications

(31 citation statements)

References 7 publications

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“…The higher respiration rate and production of osmolytes that were observed in this study are examples of how the metabolic activity of the plant is altered, reflecting the analysis of biological activity. The biological activity of carrot's respiration rate was observed using the biospeckle laser technique and showed that, in addition to other factors, respiration could be one of the causes of the changes observed in biological activity (Alves et al, 2013;Braga et al, 2005). When the plants are subjected to some form of environmental stress, biotic or abiotic, they respond by enhancing their biological activity, as their defense systems are triggered (Parida and Das, 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Rezende¹,

Soares²,

Santos³

et al. 2017

Aust J Crop Sci

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Cape gooseberry (Physalis peruviana L.) cannot tolerate high levels of salinity. Salt stress is one of the most damaging abiotic stresses that affects plant development. However, there are some evidences that silicon (Si) can act as a mitigating agent of environmental stresses. Towards understanding the stress using tissue culture, we investigated the effect of in vitro NaCl-induced salt stress in cape gooseberry and the potential mitigating effect provided by Si. Nodal segments were inoculated in MS medium with different combinations of NaCl concentrations (0.5 and 1.0% NaCl) and silicic acid (0.0, 0.5, and 1.0 g L -1 ). The experimental design was completely randomized, in a factorial design with a control treatment [(2x3) + 1], totalizing 7 treatments. The control treatment consisted of an additional treatment without the addition of any of the factors. Isoenzyme activity, proline content, CO 2 level and biological activity were assessed after 30 d. We found an increase in all antioxidant enzymes (SOD, CAT and POD) activities when salt stress was imposed, indicating that the plant has an efficient system to protect itself against stress. Among these enzymes, Si played a mitigating role only in POD activity. In relation to other evaluated enzymes, NaCl enhanced the EST and MDH activities, followed by a subsequent decrease in MDH. Si showed different performances according to the concentration of NaCl. Electrophoretic analysis represented by bands illustrated these behaviors. Proline content increased as salinity increased, and Si effect was observed by increasing the levels of this amino acid. High CO 2 level was found at the concentration of 1.0% NaCl, and Si contributed to decrease this variable to normal levels. The reduction in the respiratory rates may contribute to the allocation of carbon to other chemical reactions such as the synthesis of new tissues. Salt stress also increased the biological activity of leaves and there was no effect of Si for this variable. We conclude that salt stress causes a great damage to in vitro-grown cape gooseberry plants and the addition of 1.0 g L -1 Si can ameliorate that damage for some characteristics. However, more studies are necessary since the physiological changes played by Si have been poorly understood for dicots.

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Section: Discussionmentioning

confidence: 99%

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Rezende¹,

Soares²,

Santos³

et al. 2017

Aust J Crop Sci

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“…Les moments d'inertie mesurés, et montrant une dynamique de speckle qui diminue avec la baisse de qualité des fruits, ont été utilisés comme indicateurs de qualité et de sénescence 1 pour les fruits. Finalement, le biospeckle a été validé comme un outil pour la détection d'agents pathogènes dans les grains de café ou de blé par Braga et al [4].…”

Section: Fruits Climactériquesunclassified

Le speckle pour mesurer la maturité des fruits climactériques

Abboud

Brun

2016

Photoniques

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“…Features of seed tissue image, acquired by a CCD-camera, can be amplified and assessed by a specific image analysis software. The technique is not destructive and has been applied to test bean (Phaseolus vulgaris L.) seed viability and the presence of fungi colonies on the seed coat [18,19]. An alternative technique is the digital X-ray imaging, which was used to evaluate the effects of mechanical damages on seed quality of maize (Zea mays L.) [20].…”

Section: From Germinating Seed 2-d Images To Surface 3-d Modeling Andmentioning

confidence: 99%

New Perspectives for Seed Germination Testing Through Digital Imaging Technology

Dell’Aquila

2009

TOASJ

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Abstract:A digital image of a plant seed can be regarded as a two-dimensional object which can be measured in size, shape and color density during the development stage of germination by computer image analysis technology. Seed changes its biological structure passing from a quiescent stage to a proliferating one, and any morphological variation can be associated with the corresponding variation of seed geometry and color space components. Future perspectives are addressed to design comprehensive biological models that may contribute to studying seed quality patterns possibly integrated with those proposed for other biological systems.

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Detection of Fungi in Beans by the Laser Biospeckle Technique

Cited by 96 publications

References 7 publications

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Effects of silicon on antioxidant enzymes, CO2, proline and biological activity of in vitro-grown cape gooseberry under salinity stress

Le speckle pour mesurer la maturité des fruits climactériques

New Perspectives for Seed Germination Testing Through Digital Imaging Technology

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