Assessment of Seed Viability by Laser Speckle Techniques

Braga, Roberto A.; Fabbro, Inácio Maria Dal; Borém, Flávio Meira; Rabelo, Giovanni Francisco; Arizaga, Ricardo; Rabal, H.; Trivi, Marcelo

doi:10.1016/j.biosystemseng.2003.08.005

Cited by 117 publications

(57 citation statements)

References 16 publications

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“…The CV associated to agricultural experiments are classified as low if they are under 10%, medium when they fall between 10% and 20%, high when they are included between 20% and 30%, and very high when they are above 30%. Research work performed on biospeckle tests has shown that CV was relatively high when compared with traditional agricultural experiments, as can be seen in the report by Braga et al [1], where the obtained CV was 21.99%. Observing the statistical results presented on Table 2, it can be concluded that, in this experimental work, higher CV (25.86%) were only found for seed moisture content of 69%.…”

Section: Determination Of the Optimum Moisture Content Of Purple Ipê supporting

confidence: 55%

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Determination of the Optimum Moisture Content of Purple Ipê (Handroanthus heptaphyllus) Seeds for Biospeckle Tests

Fracarolli¹,

Enes²,

Fabbro³

et al. 2016

JAST-A

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Abstract:Handroanthus heptaphyllus (botany synonymy Tabebuia heptaphylla) is considered to have very important economical, ornamental and medicinal value. It is included in the Bignoniaceae botanical family, whose multiplication is quite solely carried by seeds. Forest dispersion elements, i.e., the seeds, deserve a deep understanding on their physiological performance. The objective of this research work was to determine the optimum seed moisture content for laser interaction in biospeckle tests, which is based on laser interferometry. Seeds were hydrated to the level of 69% and then divided into four subsamples, i.e., (1) seeds without teguments, (2) seeds with tegument, (3) seeds without teguments frozen at 0 °C and (4) seeds with teguments frozen at 0 °C. The four subsamples were then separated into five moisture level groups (28%, 35%, 54%, 63% and 69%) and exposed to the laser. The biospeckle phenomenon quantification was carried out by means of the moment of inertia (MI) approach. A positive correlation between MI and seed moisture content was obtained. It was also verified that seed moisture content between 28% and 54% showed better interaction with the laser. The results are important for the application of the biospeckle technique as a possible tool to assess the quality of purple ipê seeds.

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Section: Determination Of the Optimum Moisture Content Of Purple Ipê supporting

confidence: 55%

“…New methods of seed analysis have been discussed as novel proposals for research topics [1,2]. Nuclear magnetic resonance, near-infrared hyperspectral imaging, X-ray analysis and response to light stimuli are included among these scientific reports to assess the seed quality and viability [3][4][5][6][7].…”

mentioning

confidence: 99%

Determination of the Optimum Moisture Content of Purple Ipê (Handroanthus heptaphyllus) Seeds for Biospeckle Tests

Fracarolli¹,

Enes²,

Fabbro³

et al. 2016

JAST-A

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“…Considering a subjective biospeckle activity measurement, both inverse characteristic times show a maximum after 4 h that may be consistent with the beginning of a new physiological process inside seeds or less water flux activities in both seeds. As demonstrated in [43], it remains a relevant result as it efficiently provides information relative to the viability of a seed, only after 2 h of imbibition.…”

Section: Illustrationmentioning

confidence: 99%

Evaluation of 3D/2D Imaging and Image Processing Techniques for the Monitoring of Seed Imbibition

et al. 2018

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Seed imbibition is a very important process in plant biology by which, thanks to a simple water income, a dry seed may turn into a developing organism. In natural conditions, this process occurs in the soil, e.g., with difficult access for a direct observation. Monitoring the seed imbibition with non-invasive imaging techniques is therefore an important and possibly challenging task if one tries to perform it in natural conditions. In this report, we describe a set of four different imaging techniques that enable to addressing this task either in 3D or in 2D. For each technique, the following items are proposed. A detailed experimental protocol is provided to acquire images of the imbibition process. With the illustration of real data, the significance of the physical quantities measured in terms of their relation to the income of water in the seed is presented. Complete image analysis pipelines are then proposed to extract dynamic information on the imbibition process from such monitoring experiments. A final discussion compares the advantages and current limitations of each technique in addition to elements concerning the associated throughput and cost. These are criteria especially relevant in the field of plant phenotyping where large populations of plants are imaged to produce quantitatively significative traits after image processing.

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“…Applications in agriculture and food areas are numerous, and some additional examples include measurement of bull sperm motility, analysis of seeds (Braga et al, 2003;Vivas et al, 2017), root growth…”

Section: Analysis Of Biospeckle Laser Applicationsmentioning

confidence: 99%

When noise became information: State-of-the-art in biospeckle laser

Braga

Alves²

2017

Ciênc. agrotec.

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Laser was presented to science and industry in the 1960s and shortly became a useful tool in many areas, with applications based on its multiple characteristics such as coherence of light, which presents a phenomenon known as interference pattern, or speckle, when beam returns from an illuminated surface. Despite great application of speckle pattern, its residual presence, for example, in interferometric approaches was considered as a noise, demanding filtering. However, grains themselves became information as their dynamic changes in time started to be linked to biological sample activity. Dynamic laser speckle has been since then a phenomenon widely used to monitor biological activities in many areas from agriculture to medicine. It is known as biospeckle laser (BSL) when adopted in biological material, with high sensitivity to follow very tiny movements in biological tissues, linked to changes in speckle provided by scatterer activities inside and outside cells. Since the 1970s, biospeckle laser usage follows a crescent technologic spiral where technological developments opened room for new applications, while new demands regarding biological monitoring forced the development of new methodologies. Therefore, potential adoption of the phenomenon as a sensor, for instance, in agricultural and medical processes, as well as constant offer of new devices provided new turns in the BSL technologic spiral and opened room for technique improvement. In this study, I present a short history of biospeckle laser (BSL) with applications and development associated with challenges regarding its usage in portable and accessible devices or even in commercial equipment. And the history was packed in a temporal diagram identifying the breakpoints responsible for improvements in the use of the technique.Index terms: Optical metrology; biosystems; challenges. RESUMOO laser foi apresentado à ciência e à indústria em 1960, e rapidamente tornou-se uma ferramenta útil à muitas áreas do conhecimento, que fazem uso de suas múltiplas características, como é o caso da coerência da luz, que é responsável pelo fenômeno conhecido como padrão de interferência, ou speckle; este padrão de interferência ocorre quando a luz retorna do material iluminado formando uma figura de interferência para o observador. Apesar da grande aplicação, a presença residual do padrão de speckle em abordagens interferométricas apresentavase como um ruído que demanda filtragem. Apesar da dificuldade em alguns casos, os grãos de speckle tornaram-se fonte de informação em situações dinâmicas, quando suas mudanças começaram a ser relacionadas à atividade do material biológico iluminado. Speckle laser dinâmico tornou-se o termo usado para denominar o fenômeno que passou a ser usado para monitorar a atividade biológica e não biológica em aplicações desde a agricultura até aquelas relacionadas à medicina. Este fenômeno também é conhecido como biospeckle laser (BSL), quando aplicado em material biológico, e com grande sensibilidade para seguir os menores movimentos ...

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Assessment of Seed Viability by Laser Speckle Techniques

Cited by 117 publications

References 16 publications

Determination of the Optimum Moisture Content of Purple Ipê (Handroanthus heptaphyllus) Seeds for Biospeckle Tests

Determination of the Optimum Moisture Content of Purple Ipê (Handroanthus heptaphyllus) Seeds for Biospeckle Tests

Evaluation of 3D/2D Imaging and Image Processing Techniques for the Monitoring of Seed Imbibition

When noise became information: State-of-the-art in biospeckle laser

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