International agreements combined with the recent Chilean Forestry Policy (2015-2035) represent a challenge and an opportunity for forest landscape restoration in Chile. Nevertheless, restoring over 500,000 ha using mainly native species seems like a daunting task by 2035. Here, we discuss the three major bottlenecks that currently constrain the restoration efforts of forest ecosystems in Chile. First, Chile urgently needs a national strategic plan for forest landscape restoration, which should take into account mid-and long-term goals. There is also a need to prioritize resource allocation for efficient use, promoting the creation of economic subsidies for restoration that consider different types of forest ecosystems. Second, there is a great need for better nursery protocols in plant production as well as to strengthen educational programs for professional and technical training, given the lack of high-quality personnel in Chilean nurseries. This would help increase the currently limited national plant supply, improving quality and increasing the diversity of native species suitable for forest restoration. Lastly, taking advantage of novel eco-technological tools and promoting innovative plantation design would help to overcome the usually deficient results in the establishment phase, leading to higher survival rates and promoting better performance of native species. Beyond current experiments that are relatively efficient on a small spatial scale, the practice of forest restoration needs to become massive and successful at a landscape scale. More and better applied research is crucial for improving the impact of forest landscape restoration, so that Chile can achieve its forest restoration challenge in the next decade.

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Facing rapid loss of biodiversity as a consequence of climate change, Chile has formally pledged to restore 600,000 ha of native forest by 2035. This effort, however, has not considered the amount and quality of native plants required to meet this pledge. Thus, we examined data collected during the annual, government-conducted census of small- and medium-sized nurseries from central Chile, which account for 78% of the nation’s total plant production, to assess if current production is sufficient to meet Chile´s restoration needs. We coupled this with data collected during our series of ongoing research projects to determine if nurseries are currently meeting minimum seedling quality standards based on morpho-physiological attributes. Our four-year analysis (2016–2019) shows that the number of native seedlings has increased by only 4%, but because only 19% of nursery managers have training, just 29% of all seedlings meet quality criteria for restoration. Thus, under the current rate and quality of plant production, meeting restoration pledges desired by the year 2035 would not be achieved until 2181. This timeline can be accelerated through an urgent expansion of nursery space, implementation of a continuous program for technology and knowledge transference, and strong support through governmental policies.

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Efecto de la concentración de nitrógeno sobre atributos morfológicos, potencial de crecimiento radical y estatus nutricional en plantas de Eucalyptus globulus producidas a raíz cubierta RESUMENSe evaluó el efecto de la aplicación de cuatro concentraciones de nitrógeno (50, 100, 150 y 200 mg L -1 ) sobre atributos morfológicos, potencial de crecimiento radical y estatus nutricional de plantas de Eucalyptus globulus producidas a raíz cubierta. La frecuencia de aplicación fue semanal, manteniendo constantes los niveles de los otros macroelementos. Además, se consideró un esquema de riego al 20% de disminución de pérdida de peso de bandeja. Las variables altura de planta y área foliar alcanzaron su máximo valor al fertilizar con 200 mg L -1 , mientras que para el diámetro de cuello y número de raíces nuevas los valores obtenidos no difieren estadísticamente al fertilizar con concentraciones de 150 y 200 mg L -1 . Para el rango de concentraciones ensayadas se concluye que los mejores resultados se obtienen al fertilizar con 200 mg L -1 , ya que produce plantas con un mayor potencial de crecimiento radical y mejores atributos morfológicos, siendo la única desventaja los bajos niveles de nitrógeno foliar obtenidos al finalizar el ensayo.Palabras clave: Eucalyptus globulus, fertilización nitrogenada, estatus nutricional, atributos morfológicos.

Microbial mats are a laminated organic-sedimentary ecosystem, found in a wide range of habitats. Fluctuating diel and seasonal physicochemical gradients characterize these ecosystems, resulting in both strata and microenvironments that harbor specific microbial communities. This study was undertaken to compare two types of microbial mats across seasons to further understand the structure of fungal communities in hypersaline microbial mats and their seasonal dynamics. The structure and diversity of fungal communities was documented in young transient and mature hypersaline microbial mats from a tropical region (Puerto Rico) using one culture-dependent and three culture-independent molecular techniques based on the internal transcribed spacer (ITS) region of ribosomal DNA: terminal restriction fragment length polymorphism (TRFLP), denaturing gradient gel electrophoresis (DGGE) and clone libraries. Two microbial mats (one young and transient, one mature) were sampled in Nov 2007 (wet season), Jan 2008 (intermediate season) and Mar 2008 (dry season) in the Cabo Rojo Solar Salterns on the southwestern coast of Puerto Rico. Traditional and molecular techniques revealed strong spatial and temporal heterogeneities in both microbial mats. Higher abundance of isolates and phylotypes were observed during the wet season, and diversity decreased from the top (oxic) to the bottom (anoxic) layers in both seasons. Some of the species isolated belong to the genera Aspergillus, Cladosporium, Hortaea, Pichia and Wallemia, which often are isolated from hypersaline environments. The most abundant clones belong to Acremonium strictum and Cladosporium halotolerans, which were not isolated in pure culture. The differences observed using culture-based and molecular techniques demonstrates the need of combining methods to study the diversity of fungi in a given substrate.

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An increase in the severity of drought events on Mediterranean climates highlights the need of using plant material adapted to drought during restoration efforts. Thus, we investigated between-population morpho-physiological differences in Cryptocarya alba and Persea lingue, two native species from Mediterranean central Chile, for traits that could effectively discriminate population performance in response to water restriction (WR) testing. Three populations from each species were subjected to WR treatment and physiological, morphological, and growth parameters were assessed at the beginning and at the end of the experiment. In C. alba, the most xeric population displayed smaller plants with mesophyllous leaves and lower photosynthetic rates indicating a resource saving strategy. Moreover, the xeric population performed better during WR than the most mesic populations, exhibiting higher water use efficiency (iWUE) and maintenance of growth rates. All C. alba populations responded equally to WR in terms of morphology and biomass partitioning. In contrast, differences among P. lingue populations were subtle at the morpho-physiological level with no apparent relation to provenance environmental conditions, and no morphological traits were affected by WR. However, in response to WR application, the most mesic population was, as observed through reduction in relative growth rates, more affected than xeric populations. We attribute such discrete differences between P. lingue provenances to the lower distributional range of selected populations. Our results show that relative growth rates in both species, and iWUE only in C. alba, exhibited population specific responses upon WR imposition; these results correspond with the environmental conditions found at the origin of each populations. Both traits could further assist in the selection of populations for restoration according to their response to water stress.