Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis )

Plá, Davinia; Sanz, Libia; Sasa, Mahmood; Acevedo, Manuel; Dwyer, Quetzal; Durbán, Jordi; Pérez, Alícia; Rodríguez, Yania; Lomonte, Bruno; Calvete, Juan J.

doi:10.1016/j.jprot.2016.10.006

Cited by 49 publications

(38 citation statements)

References 82 publications

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“…Araneids seem to rely on acylpolyamines, sicariids and theridiids on proteins, and the majority of other families on peptides (Kühn‐Nentwig et al., ). A similar pattern was found in several species of snakes (Barlow et al., ; Fernández et al., ; Pla et al., ). A comparative study on 12 species of Conus snails (Phuong et al., ) also failed to find correspondence between the prey type utilized by the snails and the composition of conotoxins, identified by transcriptomic analysis.…”

Section: Discussionsupporting

confidence: 84%

Venom of prey‐specialized spiders is more toxic to their preferred prey: A result of prey‐specific toxins

Pekár

Líznarová

Bočánek

et al. 2018

Journal of Animal Ecology

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In specialized predators, a variety of adaptations have evolved to such a level of specificity that they allow very effective exploitation of focal prey. Venom is an essential adaptive trait of predatory venomous species, such as spiders, yet our knowledge of spider venom is incomplete. In agreement with the prey preference hypothesis, we expected that the venom of spider specialists should be more toxic to focal than to alternative prey, because it is composed of prey-specific toxins. Here we used spiders with three types of trophic specializations: specialists that were ant-eating, termite-eating and spider-eating. We compared the efficacy of prey capture of preferred and alternative prey (measured as paralysis latency) with that of related generalists and profiled the venom of the studied species using proteomic methods. We used 22 spider species: six myrmecophagous, two termitophagous, three araneophagous and 11 euryphagous generalist species belonging to different families. We found that ten of the eleven specialist species induced significantly shorter paralysis latency in preferred prey than in alternative prey. Generalists exhibited either similar efficiency on both prey types or slightly higher efficiency on preferred prey. Multivariate analysis of proteomic profiles (peptides and proteins) revealed significant differences between trophic specializations, particularly in peptides. Specialists appear to have venom composed of unique specific compounds as revealed by the multivariate ordination and indicator analysis. These components are likely prey-specific toxins.

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Section: Discussionsupporting

confidence: 84%

Venom of prey‐specialized spiders is more toxic to their preferred prey: A result of prey‐specific toxins

Pekár

Líznarová

Bočánek

et al. 2018

Journal of Animal Ecology

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“…Moreover, these methods also offer a means for assessing cross-recognition between particular components in the venoms of different snake species, or intraspecific variations related to geographical distribution or age [32, 50–62]. In conjunction with venomics data, antivenomics represents a significant step forward in the preclinical characterization of antivenoms, bringing further information to support decisions on the selection of venom immunogens for the production of improved antivenoms, for example.…”

Section: Antivenomics: the Immunorecognition Profiling Of Venom Antigensmentioning

confidence: 99%

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Lomonte

Calvete

2017

J Venom Anim Toxins Incl Trop Dis

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125

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This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and discusses how these may be combined through diverse experimental approaches with the goal of achieving a more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms. Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological (antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails produced by venomous snakes, responsible for thousands of deaths every year.

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“…However, defining the venom cross-reactivity landscape of an antivenom is not a trivial matter, given the well-documented occurrence of venom variability in space (intra- and interpopulation) and time (ontogenetic) within and between all taxonomic levels [19,20,21,22]. This circumstance prevents the use of phylogenetic distance as a measure of venom compositional relatedness, even between closely-related species [23].…”

Section: Introductionmentioning

confidence: 99%

Third Generation Antivenomics: Pushing the Limits of the In Vitro Preclinical Assessment of Antivenoms

Plá

Rodríguez

Calvete

2017

Toxins

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Second generation antivenomics is a translational venomics approach designed to complement in vivo preclinical neutralization assays. It provides qualitative and quantitative information on the set of homologous and heterologous venom proteins presenting antivenom-recognized epitopes and those exhibiting impaired immunoreactivity. In a situation of worrying antivenom shortage in many tropical and sub-tropical regions with high snakebite mortality and morbidity rates, such knowledge has the potential to facilitate the optimal deployment of currently existing antivenoms and to aid in the rational design of novel broad specificity antidotes. The aim of the present work was to expand the analytical capability of the immunoaffinity second-generation antivenomics platform, endowing it with the ability to determine the maximal binding capacity of an antivenom toward the different toxins present in a venom, and to quantify the fraction of venom-specific antibodies present in a given antivenom. The application of this new platform, termed third generation (3G) antivenomics, in the preclinical evaluation of antivenoms is illustrated in this paper for the case of antivenom EchiTAb-Plus-ICP® reactivity towards the toxins of homologous (B. arietans) and heterologous (N. melanoleuca) venoms.

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Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis )

Cited by 49 publications

References 82 publications

Venom of prey‐specialized spiders is more toxic to their preferred prey: A result of prey‐specific toxins

Venom of prey‐specialized spiders is more toxic to their preferred prey: A result of prey‐specific toxins

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Third Generation Antivenomics: Pushing the Limits of the In Vitro Preclinical Assessment of Antivenoms

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