The interaction between fosfomycin (FOS) and bovine serum albumin (BSA) has been investigated effectively by multi-spectroscopic techniques under physiological pH 7.4. FOS quenched the intrinsic fluorescence of BSA via static quenching. The number of binding sites n and observed binding constant KA were measured by the fluorescence quenching method. The thermodynamic parameters ΔG0, ΔH0 and ΔS0 were calculated at different temperatures according to the van’t Hoff equation. The site of binding of FOS in the protein was proposed to be Sudlow’s site I based on displacement experiments using site markers viz. warfarin, ibuprofen and digitoxin. The distance r between the donor (BSA) and acceptor (FOS) molecules was obtained according to the Förster theory. The effect of FOS on the conformation of BSA was analyzed using synchronous fluorescence spectra (SFS), circular dichroism (CD) and 3D fluorescence spectra. A molecular modeling study further confirmed the binding mode obtained by the experimental studies.
Interaction of procainamide hydrochloride (PAH) with human serum albumin (HSA) is of great significance in understanding the pharmacokinetic and pharmacodynamic mechanisms of the drug. Multi-spectroscopic techniques were used to investigate the binding mode of PAH to HSA and results revealed the presence of static type of quenching mechanism. The number of binding sites, binding constants and thermodynamic parameters were calculated. The results showed a spontaneous binding of PAH to HSA and hydrophobic interactions played a major role. In addition, the distance between PAH and the Trp–214 was estimated employing the Förster's theory. Site marker competitive experiments indicated that the binding of PAH to HSA primarily took place in subdomain IIA (Sudlow's site I). The influence of interference of some common metal ions on the binding of PAH to HSA was studied. Synchronous fluorescence spectra (SFS), 3D fluorescence spectra and circular dichroism (CD) results indicated the conformational changes in the structure of HSA.
Chloramphenicol is considered a prototypical broad band spectrum antibiotic, alongside the tetracyclines, and finds extensive applications in pharmaceuticals. Hence, its oxidation kinetic study is of much significance in understanding the mechanistic profile in biological systems. In this regard, a systematic study of oxidation of chloramphenicol (CHP) by diperiodatocuprate(III) (DPC) in the presence of micro amounts (10 −8 mol/dm 3 ) of Pd(II) and Os(VIII) catalysts has been investigated spectrophotometrically in aqueous alkaline medium at a constant ionic strength of 0.10 mol dm −3 . The reaction between CHP and DPC in alkaline medium exhibits 1:2 stoichiometry in both catalyzed reactions (CHP:DPC). The oxidation products in both reactions were found to be the same and were identified and confirmed by IR, GC-MS, and 1 H NMR. The order with respect to DPC concentration was unity, while the order with respect to CHP concentration varied from first order to zero order as the concentration of CHP increased. The rates increased with increase in [OH − ] and decreased with increase in [IO 4 − ]. The order with respect to [Pd(II)] and [Os(VIII)] was unity. It is observed that the catalytic efficiency for the title reaction is in the order of Pd(II) > Os(VIII). The catalytic constant was also determined at different temperatures for both reactions. The proposed mechanisms and the derived rate laws are in concurrence with the observed kinetics. The activation parameters with respect to the limiting step of the mechanism were calculated, and the thermodynamic quantities were also determined. Kinetic experiments suggest that [Cu(H 2 IO 6 )(H 2 O) 2 ], [Pd(OH) 2 Cl 2 ] 2− , and [OsO 4 (OH) 2 ] 2− are the reactive oxidizing species of Cu(III), Pd(II), and Os(VIII).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.