Evaluation of the binding interaction between bovine serum albumin and dimethyl fumarate, an anti-inflammatory drug by multispectroscopic methods

Multiple Spectroscopic and Theoretical Approaches to Study the Interaction between HSA and the Antiparasitic Drugs: Benznidazole, Metronidazole, Nifurtimox and Megazol

“…As can be seen in Table 1, , suggesting a high binding affinity between serum albumin and the antiparasitic drugs under study. 13,24,29,30 For BZL, MTZ and MZ samples, the decrease of K a values with the increasing of temperature indicates a decreased stability of the HSA:drug complex at a higher temperature, compatible with the static fluorescence quenching mechanism already mentioned. 13,31 On the other hand, for NFX, the increase of K a values with increasing temperature suggests that at higher temperatures the increased mobility inside the serum albumin structure allows the ligand to find improved binding within the protein pocket.…”

Synthesis, characterization and molecular docking of new C,N‐palladacycles containing pyridinium‐derived ligands: DNA and BSA interaction studies and evaluation as anti‐tumor agents

“…Förster resonance energy transfer is a non‐destructive spectroscopic method whose rate depends on the following conditions: (i) the relative orientation of the donor and acceptor transition dipoles that can produce fluorescence light with a sufficiently long lifetime and high quantum yield; (ii) the extent of overlap of fluorescence emission spectrum of the donor with the absorption spectrum of the acceptor; and (iii) the distance between the donor and the acceptor should be suitable (less than 8 nm) …”

“…[77] Förster resonance energy transfer is a non-destructive spectroscopic method whose rate depends on the following conditions: (i) the relative orientation of the donor and acceptor transition dipoles that can produce fluorescence light with a sufficiently long lifetime and high quantum yield; (ii) the extent of overlap of fluorescence emission spectrum of the donor with the absorption spectrum of the acceptor; and (iii) the distance between the donor and the acceptor should be suitable (less than 8 nm). [78] The energy transfer effect is related to the distance between the acceptor and the donor and the critical energy transfer distance, R 0 , presented by Förster's energy transfer theory, [79] and could be calculated from equation (5):…”

On the purported “backbone fluorescence” in protein three-dimensional fluorescence spectra

“…Indeed, a variability in exact peak positions and relative intensities similar to those observed in our three labs can also be noticed among the papers claiming backbone uorescence. [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] Some of the signals in the 3D uorescence spectra are not due to uorescence, but to light scattering, and can therefore be observed also for a sample that does not contain any uorophore, such as buffer or pure water. The line labelled as 'a', corresponding to the matrix diagonal, i.e.…”

“…9 Also in 2016 many examples of papers making the same claim can be found in the literature. [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] These latter references are solely meant as examples of the widespread nature of the backbone uorescence claim and are not an allinclusive list. More importantly, we would like to point out that 3D uorescence spectra are simply one of the many experiments reported in those articles.…”