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Rapid clonal micropropagation protocol of Aegle marmelos (L.) Corr. cv. CISH-B1 was achieved by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10-15th nodal region of shoots during September gave quick in vitro bud burst (5.33 days) when cultured on MS medium supplemented with BAP, 8.84 μM + IAA 5.7 μM. The maximum number of proliferated shoots (9.0/explant) were obtained on same medium supplemented with BAP 8.84 μM + IAA 5.7 μM. The micro shoots were rooted (100 %) on ½ strength MS medium supplemented with IBA 49.0 + IAA 5.7 μM. In vitro rooted plants were acclimatized on autoclaved coconut husk containing ½ strength MS plant salt mixture and under shade net house (50 % shade 70-80 % RH). The plants were established in the field after acclimatization. The micropropagated plants were tested for its genetic fidelity using 13 RAPD, 3 ISSR and 2 DAMD primers. Profile obtained by all the three Single Primer Amplification Reaction (SPAR) technique from mother tree and micropropagated plants revealed genetic integrity of micropropagated plants with that of mother tree.

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-Genetic transformation of guava (Psidium guajavaL

Although large number of varieties of guava has been selected, only few selections viz., Allahabad Safeda, Sardar, Pant Prabhat and Lalit are being exploited commercially. A medium tall less seeded, colored variety with good keeping quality and resistance to guava wilt disease is need of the day. Guava is being propagated through budding, stooling and grafting. Wilt infected mother plants of guava are playing major role in spreading the disease beyond leap and bounds. There is an urgent need to produce disease free planting material in guava. Biotechnology can help in solving some of the long-standing problems of guava cultivation. Research on guava biotechnology is still in its infancy. This paper describes current status and future need of biotechnological research for improvement of guava.