An Agrobacterium mediated transformation system of guava (Psidium guajava L.) with endochitinase gene

Mishra, Maneesh; Jalil, Syed Uzma; Sharma, Nimisha; Hudedamani, Umesh

doi:10.1590/1984-70332014v14n4a36

Cited by 20 publications

(12 citation statements)

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“…Our group developed a genetic transformation system in guava (Psidium guajava L.) using in vitro grown shoot tips as explants (Mishra et al 2014). A total of eleven transgenic guava plants introgressed with full-length Trichoderma-endochitinase gene and neomycin phosphotransferase (npt-II) gene under the control of CaMV 35S promoter and NOS terminator (Saiprasad et al 2009) were developed.…”

Section: Resultsmentioning

confidence: 99%

“…We developed two promising transgenic lines of guava expressing the Trichoderma-endochitinase gene (Mishra et al 2014). The transgenic guava plants were screened against F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

“…Chitinases have been shown to possess an antifungal role in disease resistance (Broglie et al 1991;Sela-Buurlage et al 1993). We have developed transgenic guava overexpressing the endochitinase gene derived from Trichoderma harzianum (Mishra et al 2014). A total of 11 transgenic lines have been developed.…”

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confidence: 99%

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In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii

Mishra¹,

Jalil²,

Mishra³

et al. 2016

CZECH J GENET PLANT

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Mishra M., Jalil S.U., Mishra R.K., Kumari S., Pandey B.K. (2016): In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii. Czech J. Genet. Plant Breed., 52: 6-13.Guava wilt disease is a severe threat to guava growers all over the world. It is caused by the soil-borne fungus Fusarium oxysporum f.sp. psidii. To control the disease, the Trichoderma-endochitinase gene was first introgressed into guava (Psidium guajava L.). The transgenic plantlets were screened in vitro for resistance against the wilt pathogen. Six-months-old genetically transformed plants raised in cocopeat under in vitro conditions were inoculated with a 7-days old culture of F. oxysporum f.sp. psidii. The presence of the pathogen in the cocopeat medium was confirmed by cultural as well as PCR analysis using species-specific primers. The roots of transgenic plants were wounded to facilitate the entry of the pathogen. The histopathological analysis revealed the presence of mycelium in vascular bundles. However, none of the plants showed symptoms of wilt disease during the investigation. In vitro pathogen inhibition assay and subsequently spore germination assay revealed that the crude leaf extract of transformed plants inhibited the germination of fungal conidia. The leaf tissue studied for expression of endochitinase revealed that two transgenic plants showed very high activity of N-acetyld-glucosamine (0.741 and 0.738 µM/min/µg of protein, respectively) which clearly indicated that transgenic plants could not develop any symptoms of wilt disease due to overexpression of endochitinase.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii

Mishra¹,

Jalil²,

Mishra³

et al. 2016

CZECH J GENET PLANT

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“…Cysteine, dithiothreitol (DTT), polyvinyl pyrrolidone, and ascorbic acid (Perl et al 1996, Olhoft and Somers 2001, Paz et al 2004, Frame et al 2006, Mishra et al 2014) are antioxidants that have been used to increase the potential of Agrobacterium to infect tissues by inhibiting the antimicrobial exudates produced by plant cells during the infection and cocultivation stages and/or by preventing the occurrence of necrosis in the plant tissue (Gupta 2010).…”

Section: Rav Souza Et Almentioning

confidence: 99%

Agrobacterium-mediated genetic transformation of a tropical elite maize line

Souza

Alves

Carneiro

et al. 2017

Crop Breed. Appl. Biotechnol.

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“…The unexpected frost can destroy sensitive seedlings in many fruit crops. Genetic transformation of guava with cold hardiness genes (CBF1, CBF2 and CBF3) make these plants able to tolerate cold temperatures that normally would kill unmodified seedlings (Table 1) [ [24][25][26].…”

Section: Cold Tolerancementioning

confidence: 99%

Transgenic Research in Fruit Crops: Current Status

Sharma¹,

Singh²

2016

Adv Genet Eng

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Gene mining for various biotic and abiotic stresses could be utilized only when we can transfer them to our important crops so that a large number of the population can be benefitted by this. In this light gene transfer through various methods can be done and it will be more useful where traditional breeding approach is not feasible. However, transgenic research in fruit crops is very low so that present information will be utilized by fruit breeders to a design future program for developing transgenic that ultimately open the way for those crops where gene transfer is difficult through conventional breeding.

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An Agrobacterium mediated transformation system of guava (Psidium guajava L.) with endochitinase gene

Abstract: -Genetic transformation of guava (Psidium guajavaL

Cited by 20 publications

References 21 publications

In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii

In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii

Agrobacterium-mediated genetic transformation of a tropical elite maize line

Transgenic Research in Fruit Crops: Current Status

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