In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISH-B1 through enhanced axillary branching

Pati, Rajesh; Chandra, Ramesh; Chauhan, U.K.; Mishra, Maneesh; Srivastava, Navin

doi:10.1007/s12298-008-0032-0

Cited by 26 publications

(23 citation statements)

References 33 publications

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“…The results of this molecular study revealed that all the primers used in this study showed a monomorphic pattern in all the sample plants, confirming the genetic uniformity of the regenerated plantlets and that no variation was induced during in vitro propagation of A. marina (Figures 3À5). The findings of this investigation corroborate several earlier reports on genetic analysis of regenerated plantlets in Mentha arvensis, [16] Aegle marmelos, [27] Bambusa balcooa, [28] Gerbera jamesonii [29] and Gloriosa superba [30], using SPAR analysis.…”

Section: Genetic Fidelity Of Regenerated Plantssupporting

confidence: 91%

Clonalin vitromultiplication of grey mangrove and assessment of genetic fidelity using single primer amplification reaction (SPAR) methods

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Faisal

Hegazy

et al. 2015

Biotechnology & Biotechnological Equipment

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An efficient protocol for clonal multiplication of an important mangrove, Avicennia marina, was developed through in vitro culture of nodal segments obtained from a mature plant. The nodal explant induced multiple shoots when cultured on the Murashige and Skoog (MS) basal medium supplemented with varying concentrations and combinations of 6-benzyladenine (BA) and a-naphthalene acetic acid. The highest response in terms of per cent regeneration (73%), average number of shoots/explant (3.25 § 0.25) and maximum shoot length (5.2 § 0.27 cm) was obtained on the MS medium supplemented with BA 5.0 mmol/L C NAA 1.0 mmol/L C 3 g/L activated charcoal after 8 weeks of culturing. The regenerated shoots were rooted well in the MS medium supplemented with 1.0 mmol/L indole-3-butyric acid with an average of 2.9 § 0.24 roots per microshoot. The rooted plantlets were successfully transferred to pots containing normal garden soil with 70% survival rate. The genetic stability of the regenerated plants was evaluated using single primer amplification reaction (SPAR) methods viz., random amplified polymorphic DNA, directed amplification of minisatellite DNA and intersimple sequence repeat polymorphism. The SPAR analysis revealed monomorphic banding patterns in all in vitro regenerated plantlets of A. marina and similar with that of the mother tree confirming their genetic uniformity and clonal fidelity.

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Section: Genetic Fidelity Of Regenerated Plantssupporting

confidence: 91%

Clonalin vitromultiplication of grey mangrove and assessment of genetic fidelity using single primer amplification reaction (SPAR) methods

Alatar

Faisal

Hegazy

et al. 2015

Biotechnology & Biotechnological Equipment

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“…Earlier reports demonstrated that plant regeneration was possible through apical shoot bud, node, internodes, leaf and nucellar tissues explants (Pati et. al., 2008;Arumugam et.…”

Section: Resultsmentioning

confidence: 99%

“…al., 1982). The tree grows wild in dry forests on hills and plains of central Southern India, Burma, Pakistan, Bangladesh, Srilanka, Northern Malaya, Java and Philippine Islands (Pati et. al., 2008).…”

Section: Introductionmentioning

confidence: 99%

In vitro clonal multiplication of Aegle marmelos (L.) Corr. through cotylodonary node culture

Akter

Banu

Habib

et al. 2013

Bangladesh J. Sci. Ind. Res.

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Clonal multiplication of Aegle marmelos was achieved through in vitro culture. Cotyledonary nodes from one month old in-vitro grown seedlings of Aegle marmelos were cultured on MS medium supplemented with BAP, Kn, and IBA either alone or in combination. The highest regenerative response was observed on medium containing 2.5 mg/L BAP and 0.5 mg/L Kn from node and shoot tip within 8-10 days. When regenerated shoots were subcultured on MS medium supplemented with 0.2 mg/L BAP and 0.02 mg/L Kn, maximum number of multiple shoots were observed after third phase of subculture. Poor response was found using MS medium supplemented with Kn only. In vitro induced shoots were transferred into root induction medium consisting of half-strength MS supplemented with auxins, IAA, IBA or NAA. Rooting was best in medium supplemented with 1.0 mg/L IBA. Rooted plantlets were acclimatized and transferred to the soil with 96% survival rate. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15408 Bangladesh J. Sci. Ind. Res. 48(1), 13-18, 2013

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“…Micropropagation protocol of Pati et al (2008a;2008b) was followed to regenerate plantlets of Aegle marmelos Corr.…”

Section: Explant and Growth Conditionsmentioning

confidence: 99%

“…Micropropagation technique has been developed in Aegle mormelos Corr. from mature tree through enhanced axillary branching for mass multiplication (Pati et al, 2008;Pati et al, 2008a;Pati et al, 2008b;Raj and Basavaraju, 2012). However, high mortality of micropropagated plants during acclimatization and subsequently poor field establishment remains a major bottleneck in commercealizing the micropropagation protocol to industry.…”

Section: Introductionmentioning

confidence: 99%

Histological and Biochemical Changes in <i>Aegle marmelos</i> Corr. before and after Acclimatization

Rajesh¹

2013

tgmb

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Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage, and field established plants). The biochemical result showed that micropropagated plantlets produced significantly low total chlorophyll (0.042 mg/g fresh weight), reducing sugar (3.227%), NR activity (1.353 NO 2 /h/g fresh weight) and but higher protein (0.048 µg/g) during in vitro phase. The in vitro raised plants showed abnormal histological features like altered leaf mesophyll, absence of thick cuticle, sunken stomata, poorly developed stem and root histology. These results revealed that the photoautrophic mode of nutrition during in vitro phase increased the survival rate during acclimatization compared to photoheterotrophic mode of nutrition. This suggests that photoautotrophism phenemoneon has substantial influence on the physiology and development of in vitro regenerated Aegle marmelos Corr. plantlets.

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In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISH-B1 through enhanced axillary branching

Cited by 26 publications

References 33 publications

Clonalin vitromultiplication of grey mangrove and assessment of genetic fidelity using single primer amplification reaction (SPAR) methods

Clonalin vitromultiplication of grey mangrove and assessment of genetic fidelity using single primer amplification reaction (SPAR) methods

In vitro clonal multiplication of Aegle marmelos (L.) Corr. through cotylodonary node culture

Histological and Biochemical Changes in <i>Aegle marmelos</i> Corr. before and after Acclimatization

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