This study was conducted to investigate the Cytomegalovirus (CMV) infections of pregnant women. The samples were collected from Mosul and Baghdad hospitals in Iraq for two years and the tested women within the age category of under 20 to above 39 years. One thousand five hundred samples were taken as serum, to use in ELISA (IgM, IgG). EDTA blood, Heparin blood and cervical swabs were used in molecular tests. Three hundred positive samples demonstrating the presence of IgM and IgG antibodies using ELISA test. IgM antibodies were positive in 146 (48.7%) CMV, IgG antibodies were positive in 189 (63%) for CMV. DNA was extracted and Real-Time PCR indicates positive in only four samples (1.3%) in CMV from all 300 positive samples in ELISA tests.

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Hyperimmunized egg yolk (HIY) was produced in two layer hens by four successive immunization with live attenuated Newcastle disease vaccine (NDV) by seven days intervals for each vaccination process. Fifty broiler chicks were used for treatment with hyperimmunized yolk. They were divided into five groups. The first group was treated at 14th days of age by orally and intramuscular injection (five chicken for each route). The second group was boostered with (HIY) after 7 days of first dose at14 days of age. The third group as that of the first group but treated at 21 st day of age. The fourth group was treated at 21 st days and boostered after 7 days. The fifth group was ten chicks remain without any treatment used as control comparison of all groups. Immune response was measured using HI technique.The results showed that the group of 14 th day of age with booster dose gave high antibody titer by intramuscular injection (second group).

The present work aimed to study the pathogenicity of Archanobacterium pyogenes by using one day old chicks and chicken Embryonated eggs .The chicks were given the bacteria(4.2x10 8 CFU/ml) by orally administration and intrapertoneal injection, while the Embryonated eggs inoculated into allontoic sac at 12 days of incubation. The chicks were show dullness, decrease in food intake and nervous sings, most chicks were died after 2-3 days, and the bacteria were reisolated from internal organs, and yolk sac. The inoculation of Embryonated chicken eggs resulting in embryonicdeath, reduce hatchery percentage and deformity of embryo. The study showed that A. pyogenesisvirulence to both chicken Embryonated eggs and one day old chicks.

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Background: TORCH complex (Toxoplasma gondii, others, Rubella virus, Cytomegalovirus, and Herpes simplex virus) infections in pregnant women may attribute to bad obstetric outcomes.Objective: To investigate the role of TORCH as an etiology of bad obstetric outcome using a molecular technique.Materials and Methods: The samples were collected from women with Bad Obstetric History attending clinics in Mosul and Baghdad hospitals in Iraq over a period from (15/4/2013) to (1/6/2014) and from (1/5/2017) to (1/11/2017). The women included in the study were with mean age of (26±6.1) years and a range of 22 to 39 years. Blood samples, throat and cervical swabs were collected from 300 women ELISA positive seroprevalence of TORCH for PCR testing.Results: DNA and RNA were extracted and Real-Time PCR indicates negative results for T.gondii, Rubella and HSV I&II, but were CMV positive in only four samples represented (1.3%) from total 300 positive samples in ELISA tests.Conclusion: ELISA test is considered as a preliminary and screening test for TORCH infections. Real Time PCR is an essential tool in the research laboratory. It has engendered wider acceptance than the conventional PCR due to its improved rapidity, sensitivity, reproducibility and the reduced risk of carry-over contamination