This study was conducted to investigate the Cytomegalovirus (CMV) infections of pregnant women. The samples were collected from Mosul and Baghdad hospitals in Iraq for two years and the tested women within the age category of under 20 to above 39 years. One thousand five hundred samples were taken as serum, to use in ELISA (IgM, IgG). EDTA blood, Heparin blood and cervical swabs were used in molecular tests. Three hundred positive samples demonstrating the presence of IgM and IgG antibodies using ELISA test. IgM antibodies were positive in 146 (48.7%) CMV, IgG antibodies were positive in 189 (63%) for CMV. DNA was extracted and Real-Time PCR indicates positive in only four samples (1.3%) in CMV from all 300 positive samples in ELISA tests.

Serological detection of IgG antibodies to antigens one soil isolate 0f thermophilic actinomycetes belonged to the species Thermoactinomyces vulgaris in (70) sera of patients with farmer's lung disease and hay fever patients compared with control normal peoples in the same environmental condition were carried out using ELISA method. The results indicated that (19) sera from symptomatic patients having IgG level between (1.007-1.626) I.U/ml, four of them having farmer's lung disease and the remaining have hay fever as diagnosed by clinical. The IgG level between (0.293-0.944) I.U/ml indicated negative and which was found in(16) patients two of them having farmer's lung disease and the remaning have hay fever. The IgG level of normal people were devided in two groups: first account positive control was between (1.089-2.147) I.U/ml and the second account negative was between (0.819-0.959) I.U/ml. The present study has demonstrated the usefulness of estimation of specific IgG antibodies activity to thermophilic actinomyces by ELISA for the diagnosis of farmer's lung disease as a screening test of large number of samples with sufficient high sensitivity.

Background: TORCH complex (Toxoplasma gondii, others, Rubella virus, Cytomegalovirus, and Herpes simplex virus) infections in pregnant women may attribute to bad obstetric outcomes.Objective: To investigate the role of TORCH as an etiology of bad obstetric outcome using a molecular technique.Materials and Methods: The samples were collected from women with Bad Obstetric History attending clinics in Mosul and Baghdad hospitals in Iraq over a period from (15/4/2013) to (1/6/2014) and from (1/5/2017) to (1/11/2017). The women included in the study were with mean age of (26±6.1) years and a range of 22 to 39 years. Blood samples, throat and cervical swabs were collected from 300 women ELISA positive seroprevalence of TORCH for PCR testing.Results: DNA and RNA were extracted and Real-Time PCR indicates negative results for T.gondii, Rubella and HSV I&II, but were CMV positive in only four samples represented (1.3%) from total 300 positive samples in ELISA tests.Conclusion: ELISA test is considered as a preliminary and screening test for TORCH infections. Real Time PCR is an essential tool in the research laboratory. It has engendered wider acceptance than the conventional PCR due to its improved rapidity, sensitivity, reproducibility and the reduced risk of carry-over contamination

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