Background: Candida is one of the commensals housing the normal oral flora. Oral candidiasis is a common opportunistic infection caused by Candida species (Candida albicans being the most common one). Green tea is well-known for its various health benefits, and the literature reveals ongoing studies on the action mechanisms of green tea extract, in vitro and in vivo. Aims and Objectives: The present study was undertaken to assess antifungal effect of green tea extracts; if proven, could it be used in future as an adjunctive treatment modality for treating candidiasis. Materials and Methods: About 15 healthy volunteers with coated tongue were included for the study. Oral sample, from dorsum of the tongue was collected by commercially available pre-sterilized swabs. The material was inoculated into Sabouraud's Dextrose Agar; confirmed for the presence of Candida species. The sensitivity test was carried out on a lawn culture with 25% and 50% green tea extracts at 24 h and 48 h intervals. The antifungal activity was assessed. Distilled water was used as a control. Student's paired t-test was used to assess the difference in inhibition effect between 25% and 50% of green tea extract solution. Results: Statistically significant increase in the zone of inhibition was noticed at both 25% and 50% concentration of green tea extracts when compared to control group, which did not show any zone of inhibition. Conclusion: Having shown antifungal effects, the green tea extracts may be considered in future as a therapeutic adjunct.
Cucurbits belonging to family Cucurbitaceae or the gourd family of flowering plants, are very important nutritionally. After harvest, storing of these cucurbits in optimal conditions is important to ensure that the nutrients are not lost. In the present investigation, an attempt was made to study the effect of various storage conditions on the nutrient content of three most commonly growing and easily available cucurbits, Cucumis sativus L. (Cucumber), Momordica charantia L. (Bitter gourd) and Luffa acutangula L. (Shiradi, Luffa). It was observed that protein content, vitamin C, iron and crude fiber content in all the cucurbits studied showed a gradual reduction with increase in days of storage. Temperature of storage 0 was also found to be an important factor for the nutrients to be maintained in Cucurbits. Storage at 15 C was found to be 0 better as the decrease in nutrients was found to be minimum when the fruits were stored at 15 C compared to storage at 0 room temperature and 5 C
Background: Xylene is used as a clearing and dewaxing agent during routine tissue processing in histopathology laboratories. Despite its excellent clearing property, xylene is associated with adequate amount of toxicity. Therefore, various substitutes for xylene such as mineral oil and vegetable oil mixtures have been explored and are well documented in English literature. Aims and Objectives: This study had aimed at assessing the efficacy of bleached vegetable oil as a clearing and a dewaxing agent. The objectives were to compare the clearing ability of bleached vegetable oil with that of xylene. Materials and Methods: A total of 12 normal oral mucosal samples (n = 12) were cut into pairs forming two groups -Group A tissues (n = 12) were processed in xylene and Group B tissues (n = 12) were processed in bleached vegetable (palm) oil. The parameters such as transparency of the tissues, nuclear and cytoplasmic staining were assessed between the two groups. Results: Tissues from both the study groups were transparent and had yielded good serial sections; adequate nuclear and cytoplasmic staining was observed in 91.7% of sections treated with bleached palm oil.
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