Bleached vegetable oil as a suitable bio-safe alternative to xylene: An exploratory study

Abstract

Background: Xylene is used as a clearing and dewaxing agent during routine tissue processing in histopathology laboratories. Despite its excellent clearing property, xylene is associated with adequate amount of toxicity. Therefore, various substitutes for xylene such as mineral oil and vegetable oil mixtures have been explored and are well documented in English literature. Aims and Objectives: This study had aimed at assessing the efficacy of bleached vegetable oil as a clearing and a dewaxing agent. The objectives were to compare the clearing ability of bleached vegetable oil with that of xylene. Materials and Methods: A total of 12 normal oral mucosal samples (n = 12) were cut into pairs forming two groups -Group A tissues (n = 12) were processed in xylene and Group B tissues (n = 12) were processed in bleached vegetable (palm) oil. The parameters such as transparency of the tissues, nuclear and cytoplasmic staining were assessed between the two groups. Results: Tissues from both the study groups were transparent and had yielded good serial sections; adequate nuclear and cytoplasmic staining was observed in 91.7% of sections treated with bleached palm oil.

PENGARUH PENGGUNAAN MINYAK ZAITUN DENGAN PEMANASAN SEBAGAI LARUTAN PENJERNIH (CLEARING) TERHADAP KUALITAS SEDIAAN JARINGAN HEPAR MENCIT (Mus musculus)

“…Tahap penjernihan (clearing) merupakan salah satu langkah penting dalam histoteknik. Xylene digunakan sebagai larutan rutin penjernih, namun paparan jangka pendek xylene dapat merusak sistem tubuh manusia dan memiliki efek toksik bagi penggunanya (Alwahaibi et al, 2018) karena itu banyak penelitian terdahulu mengidentifikasi kemampuan minyak nabati yang lebih safety sebagai alternatif pengganti xylene (Madhura et al, 2016).…”