Necrotic lesions surrounded by yellow areas on leaves followed by stem necrosis were observed on chrysanthemum plants cultivated in Atibaia, Sào Paulo State, Brazil. The host range, in vitro properties and particle morphology of the causal virus were typical of a Tospovirus. Serological studies demonstrated that the virus differed from tomato spotted wilt. tomato chlorotic spot, groundnut ringspot and inpatiens necrotic spot Tospoviruses. The virus isolate is thus possibly a representativeof a new serogroup of a new virus.

Badnavirus in Bougainvillea spectabilis showing virus-like symptoms was identified by the presence of bacilliform particles, measuring 125-130 ¥ 30-40 nm in leaf-dip preparations and by analysis of its putative open reading frame 3 sequence. The virus, tentatively named Bougainvillea bacilliform virus (BBV), had the highest identities (up to 60%) with Spiraea yellow leaf spot virus, Gooseberry vein banding associated virus, Taro bacilliform virus, and Citrus yellow mosaic virus. In phylogenetic analysis, BBV clustered with Badnavirus putative species. Attempts to transmit the virus to several hosts failed. This is the first report of a new Badnavirus detected in Bougainvillea.

,3 milhões. Este trabalho apresenta os resultados de um levantamento feito nos mercados de flores do estado de São Paulo, somados a dados e informações obtidas junto aos principais produtores de bulbosas ornamentais. As principais espé-cies cultivadas e comercializadas no País como flores de corte e vaso são: alstroeméria (Alstroemeria sp.); amarílis (Hyppeastrum sp.); copo-de-leite (Zantedeschia sp.); gladíolo (Gladiolus X grandiflorus); hemerocale (Hemerocallis sp.) e lírio (Lilium sp.). Observou-se que a produção e, consequentemente, o mercado é crescente, porém o País enfrenta um grande entrave interno com relação à legislação que regulamenta a proteção de cultivares e o uso de defensivos químicos. Palavras-chave: mercado, produção, alstroeméria (Alstroemeria sp.), amarílis (Hyppeastrum sp.), copo-de-leite (Zantedeschia sp.), gladíolo (Gladiolus X grandiflorus), hemerocale (Hemerocallis sp.) ABSTRACT Ornamental bulbs in BrazilThe ornamental bulbs market in Brazil, in 2010, moved a total value of exports of U.S. $ 13-14 million and imported U.S. $ 7.3 million. This paper presents the results of a survey from the flower markets of São Paulo, in addition to data and information obtained from the major producers of ornamental bulbs. The main species cultivated in Brazil and marketed as cut flowers and pot are: Alstroemeria sp., Hyppeastrum sp., Zantedeschia sp., Gladiolus X grandiflorus, Hemerocallis sp. and Lilium sp. It was noted that production and, consequently, the market is growing, but the country faces a major obstacle with respect to the internal laws governing the protection of plant cultivars and the use of chemical pesticides.

Petunia plants collected in SaÄ o Paulo City, Brazil, showing yellow mosaic, were naturally infected by a virus of the genus Tobamovirus identi®ed according to particle morphology and size, host range, physical properties and cytopathic eects. On the basis of serological properties, amino acid composition and nucleotide sequence of the coat protein gene, the virus isolate was identi®ed as a new strain of Tobacco mosaic virus (TMV-p). A conspicuous feature of this virus infection is the presence of virus-like particles within the mitochondrial matrix. The data from phylogenetic analysis indicate that TMV-p belongs to subgroup 1 of the genus Tobamovirus.

The complete nucleotide sequence of an isolate of tomato mottle mosaic virus (ToMMV) was determined. The virus, originally isolated from symptomatic tomato plants found in a county near the city of São Paulo, Brazil, has a genome with 99% nucleotide sequence identity with ToMMV from Mexico, China, Spain, and the United States.

Petunia plants from a nursery in the State of Rio Grande do Sul, Brazil, showed pronounced vein banding and contained isometric particles with diameters of approximately 45 and 30 nm. The larger ones apparently represent a caulimovirus, while the smaller ones, which included both empty shells and full particles, were identified as those of a new tymovirus for which we propose the name Petunia vein banding virus (PetVBV). Originally, PetVBV was transmitted only with difficulty to healthy petunia plants. However, from an experimentally infected petu-nia, it was later readily transmitted also to Nicotiana benthamiana and Nicandra physalodes, but not to other species in the Solanaceae or other plant families. It produces cytopathic effects typical for tymovirus infections. Its coat protein shows approximately 65% amino acid sequence identity with those of Eggplant mosaic and Andean potato latent viruses, to which it is also serologically more closely related than to any other tymoviruses.

Mosaic symptoms in Mirabilis jalapa plants from a public garden in the city of São Paulo, Brazil, were associated with the presence of Tomato chlorotic spot virus (TCSV-Mir-BR07). TCSV-Mir-BR07 was mechanically transmitted to healthy M. jalapa plants. Phylogenetic analysis revealed a monophyletic group formed by South American TCSV isolates separate to those from Central and North America. This is the first report of natural TCSV infection in M. jalapa.

The culture and commercialization of ornamental plants have considerably increased in the last years. To supply the commercial demand, several Hemerocallis and Impatiens varieties have been bred for appreciated qualities such as flowers with a diversity of shapes and colors. With the aim of characterizing the tobamovirus isolated from Hemerocallis sp. (tobamo-H) and Impatiens hawkeri (tobamo-