A new Badnavirus species detected in Bougainvillea in Brazil

Rivas, Eliana Borges; Duarte, L. M. L.; Alexandre, M. A. V.; Fernandes, Flora Maria de Campos; Harakava, Ricardo; Chagas, C. M.

doi:10.1007/s10327-005-0227-y

Cited by 11 publications

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References 4 publications

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“…2). Previously, bacilliform virus particles have been shown to be associated with bougainvillea plants with CVB in Brazil (Rivas et al 2005) and mottling, chlorosis, vein-banding and stunting in Taiwan (Tsai et al 2005).…”

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First report of two distinct badnaviruses associated with Bougainvillea spectabilis in India

2010

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Two distinct types of symptoms, severe yellow mosaic and chlorotic vein-banding, were observed on Bougainvillea spectabilis at two different locations in India. Electron microscopy revealed bacilliform virus particles in leaf samples from both places. PCR amplification, cloning, sequencing and sequence analysis of reverse transcriptase and ribonuclease H coding region in open reading frame 3 indicated that two distinct badnaviruses are associated with B. spectabilis in India.Keywords Badnavirus Á Bougainvillea spectabilis Á Delhi Á Tirupati Á PCR Bougainvillea spectabilis Willd (flower paper) is a widely grown ornamental plant in India. During 2008, B. spectabilis plants developed distinct severe yellow mosaic (SYM) in parts of Tirupati (Fig. 1a). Subsequently in 2009, a chlorotic vein-banding (CVB) was noticed on leaves of B. spectabilis on the campus of the Indian Agricultural Research Institute, New Delhi (Fig. 1b). Leaf dip electron microscopy of symptomatic bougainvillea leaves revealed the association of bacilliform virus particles measuring 120-150 nm 9 20 nm (Fig. 2). Previously, bacilliform virus particles have been shown to be associated with bougainvillea plants with CVB in Brazil (Rivas et al. 2005) and mottling, chlorosis, vein-banding and stunting in Taiwan (Tsai et al. 2005).To confirm the virus identity, symptomatic leaves of bougainvillea collected from Tirupati and Delhi were subjected to polymerase chain reaction (PCR) amplification using two degenerate Badnavirus primers viz. BadnaFP (5 0 ATGCCITTYGGIAARAAYGCICC3 0 ) and BadnaRP (5 0 CCAYTTRCAIACISCICCCCAICC3 0 ) corresponding to putative ribonuclease H (RNase H) and reverse transcriptase (RT) coding regions (Yang et al. 2003). Both samples yielded an amplicon of approximately 600 bp, and no amplification was observed in healthy, symptomless bougainvillea (Fig. 3). The amplicons were cloned in pGEM-T easy vector (Promega, Madison, WI, USA) and sequenced. BLAST analysis of the 577 bp of RNase H and RT nucleotide sequences of viral DNA from bougainvillea with SYM symptoms from Tirupati (accession GQ254410) had maximum sequence identity of 73% in open reading frame 3 (ORF 3) of Bougainvillea spectabilis chlorotic vein-banding virus (BsCVBV) (accessions EU034539, DQ347841, and DQ103759). An identical sequence size of amplified viral DNA isolated from bougainvillea with CVB symptoms from Delhi (accession GQ254411) had 72% sequence identity with the sequences of same three BsCVBV isolates. Nucleotide and amino acid sequences of putative RT and RNase H region of ORF 3 of these two badnaviruses were analyzed using Bio Edit version 7.0.4 for sequence identities with other badnaviruses. The two badnaviruses infecting bougainvillea in India shared an identity of 76.2% in nucleotide and 88.6% in amino acid sequences with each other. Comparison of nucleotide and amino acid sequences of badnaviruses infecting bougainvillea from India and Taiwan showed identities in the range of 72.3-73.5% for nucleotides and 83.4-84.9% in amino acids of RT and RNase H. Be...

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First report of two distinct badnaviruses associated with Bougainvillea spectabilis in India

2010

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“…In addition, bougainvillea badnaviruses are not transmitted by mechanical inoculation (Rivas et al. ; Tsai et al. ).…”

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“…This discovery was later confirmed based on sequence homology with badnaviruses (Rivas et al. ). Yamashita et al.…”

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confidence: 87%

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Genetic Diversity of Badnaviruses Associated with Bougainvillea in Brazil

Alexandre

Duarte

Harakava

2015

Journal of Phytopathology

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The genus Bougainvillea is native to South America and is cultivated worldwide for ornamental purposes. To investigate the diversity of bougainvillea badnavirus populations, plants with various types of symptoms from different regions of Brazil were collected and subjected to DNA extraction, PCR, cloning and sequencing. Sequence analysis revealed that clones from the Bras ılia (DF) isolate showed maximum identity of 71.7%. No genetic diversity was observed in partial RT/RNase H sequences of Badnavirus isolates derived from the bougainvillea clones grown in São Paulo (SP) state, which shared a monophyletic group with 100% bootstrap in the tree topology reconstructed by maximum likelihood. One clade consistently supported by 100% bootstrap formed by clones of the samples from Minas Gerais (MG) and Cear a (CE) states and Taiwan was also observed. Furthermore, DF, CE and MG isolates formed a monophyletic group with the Delhi isolate, which may represent a new Badnavirus species that infects bougainvillea. Two Brazilian isolates and one from Taiwan appeared to have recombination sites in the RT and RNase H conserved region of BCVBV. These results demonstrate the existence of at least three probably new bougainvillea badnavirus species or strains, which may be spread, as this ornamental is vegetatively propagated.

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“…No Brasil, uma doença conhecida como clareamemento de nervuras tem sido atribuída a um Badnavirus, tanto na espécie B. spectabilis (CHAGAS et al, 2001) como em B. glabra (YAMASHITA et al, 2004). Em 2005, foi relatado um novo Badnavirus (Bougainvellea bacilliform virus) causando sintomas de manchas cloróticas em folhas de B. spectabilis (RIVAS et al, 2005). Há relatos na literatura da ocorrência de vírus (Impatiens necrotic spot vírus) causando sintomas de amarelecimento e manchas nas folhas, bem como clareamento e necrose de nervuras (SHAHRAEEN, GHOTBI, MEHRABAN, 2002).…”

Section: Doenças Infecciosas Da Primaveraunclassified

Identificação de fitoplasmas associados à síndrome do amarelecimento foliar da cana-de-açúcar e ao superbrotamento de primavera (Bougainvillea spectabilis) no estado de São Paulo

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A new Badnavirus species detected in Bougainvillea in Brazil

Cited by 11 publications

References 4 publications

First report of two distinct badnaviruses associated with Bougainvillea spectabilis in India

First report of two distinct badnaviruses associated with Bougainvillea spectabilis in India

Genetic Diversity of Badnaviruses Associated with Bougainvillea in Brazil

Identificação de fitoplasmas associados à síndrome do amarelecimento foliar da cana-de-açúcar e ao superbrotamento de primavera (Bougainvillea spectabilis) no estado de São Paulo

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