First Complete Genome Sequence of an Isolate of Tomato Mottle Mosaic Virus Infecting Plants of Solanum lycopersicum in South America

Nagai, Alice; Duarte, L. M. L.; Chaves, Alexandre Levi Rodrigues; Alexandre, M. A. V.; Ramos-González, Pedro Luis; Chabi-Jesus, Camila; Harakava, Ricardo; Santos, Déborah Yara Alves Cursino dos

doi:10.1128/genomea.00427-18

Cited by 10 publications

(7 citation statements)

References 11 publications

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“…ToMMV has already been reported from Spain (Ambrós et al, 2017) and the Netherlands (Fowkes et al, 2022) but to our knowledge this is the first report of ToMMV in seeds produced in France. The results of this study, together with the report on its presence in a sample misidentified as ToMV in the past (Nagai et al, 2018), indicate that ToMMV had already been present before its first description and is possibly more widespread than currently known.…”

supporting

confidence: 49%

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Identification of Tomato mottle mosaic virus in historic seed accessions originating from France, the Netherlands and Spain, indicates a wider presence before its first description

et al. 2023

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Tomato mottle mosaic virus (ToMMV) is a tobamovirus found in a Solanum lycopersicum sample collected in Mexico in 2009. To assess the possible presence of ToMMV in Europe, accessions from a historic seed collection were tested by real-time RT-PCR and Illumina sequencing. ToMMV was identified in historical seed accessions produced in France, the Netherlands and Spain. Three different near complete genome sequences were obtained, each corresponding to the country in which the seeds had been produced. Positive samples from France and Spain could be related to the same production location and year, respectively, while the identical genome sequences from the Netherlands were obtained from samples produced in different locations and years between 1981 and 2007. The latter could be due to the fact that the Dutch seed accessions had been repacked in the past at the same location and time as accessions with a relatively high virus load from 2007. This indicates that possibly only the seeds from 2007 originated from ToMMV-infected plants, while the detection of ToMMV in the older seed accessions resulted from cross contamination. This data shows that ToMMV has been around in Europe before its first description and is possibly more widespread than currently known.

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supporting

confidence: 49%

“…plants collected in Brazil in 1992, for instance, were not reported before 2018 (Nagai et al, 2018). To gain more insight into its presence in Europe, historical seed accessions of S. lycopersicum and Capsicum spp.…”

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confidence: 99%

Identification of Tomato mottle mosaic virus in historic seed accessions originating from France, the Netherlands and Spain, indicates a wider presence before its first description

et al. 2023

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“…It was first reported in Mexico in 2013, on a sample collected in 2009 [21]; the associated disease is characterized by symptoms of plant stunting, severe mosaic, mottling, distortion, and necrosis on leaves, and fruit necrosis in tomato [21][22][23]; in pepper, symptoms such as apical yellowing and necrosis were observed [22,24]. After the first report, ToMMV has been detected in the United States [25], Spain [24], Israel [26], China [27], Brazil [28], and Czech Republic [29]. Pepper seed lots imported into Australia were also found to be infected by ToMMV [30].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a One-Step Reverse Transcription Real-Time PCR Assay for Simultaneous Detection and Identification of Tomato Mottle Mosaic Virus and Tomato Brown Rugose Fruit Virus

Tiberini¹,

Manglli²,

Taglienti³

et al. 2022

Plants

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Tobamovirus species represent a threat to solanaceous crops worldwide, due to their extreme stability and because they are seed borne. In particular, recent outbreaks of tomato brown rugose fruit virus in tomato and pepper crops led to the establishment of prompt control measures, and the need for reliable diagnosis was urged. Another member of the genus, tomato mottle mosaic virus, has recently gained attention due to reports in different continents and its common features with tomato brown rugose fruit virus. In this study, a new real-time RT-PCR detection system was developed for tomato brown rugose fruit virus and tomato mottle mosaic virus on tomato leaves and seeds using TaqMan chemistry. This test was designed to detect tomato mottle mosaic virus by amplifying the movement protein gene in a duplex assay with the tomato brown rugose fruit virus target on the CP-3’NTR region, which was previously validated as a single assay. The performance of this test was evaluated, displaying analytical sensitivity 10−5–10−6-fold dilution for seeds and leaves, respectively, and good analytical specificity, repeatability, and reproducibility. Using the newly developed and validated test, tomato brown rugose fruit virus detection was 100% concordant with previously performed analyses on 106 official samples collected in 2021 from different continents.

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“…Spain [26], Israel [28], China [29], Brasil [30] and Czech Republic [31]. Pepper seed lots imported in Australia were also found infected by ToMMV [32].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a One-Step Reverse Transcription Real-Time PCR Assay for Simultaneous Detection and Identification of Tomato Mottle Mosaic Virus and Tomato Brown Rugose Fruit Virus

Tiberini¹,

Manglli²,

Taglienti³

et al. 2021

Preprint

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Tobamovirus species represent a threat to solanaceous crops worldwide, due to their extreme stability and being seed-borne. In particular, recent outbreaks of tomato brown rugose fruit virus in tomato and pepper crops led to the establishment of prompt control measures, and the need for reliable diagnosis was urged. Another member of the genus, tomato mottle mosaic virus, has recently risen attention due to reports in different continents and its common features with tomato brown rugose fruit virus. In this study, a new real-time RT-PCR detection system was developed for tomato brown rugose fruit virus and tomato mottle mosaic virus on tomato leaves and seeds using TaqMan chemistry. This test was designed to detect tomato mottle mosaic virus by amplifying the movement protein gene in a duplex assay with tomato brown rugose fruit virus target on the CP-3’NTR region, which was already validated as a single assay. The performance of this tests was evaluated, displaying analytical sensitivity 10-5-10-6-fold dilution for seeds and leaves, respectively, and good analytical specificity, repeatability, and reproducibility. Using the newly developed and validated test, tomato brown rugose fruit virus detection was 100% concordant with previously performed analyses on 106 official samples collected in 2021 from different continents.

show abstract

First Complete Genome Sequence of an Isolate of Tomato Mottle Mosaic Virus Infecting Plants of Solanum lycopersicum in South America

Cited by 10 publications

References 11 publications

Identification of Tomato mottle mosaic virus in historic seed accessions originating from France, the Netherlands and Spain, indicates a wider presence before its first description

Identification of Tomato mottle mosaic virus in historic seed accessions originating from France, the Netherlands and Spain, indicates a wider presence before its first description

Development and Validation of a One-Step Reverse Transcription Real-Time PCR Assay for Simultaneous Detection and Identification of Tomato Mottle Mosaic Virus and Tomato Brown Rugose Fruit Virus

Development and Validation of a One-Step Reverse Transcription Real-Time PCR Assay for Simultaneous Detection and Identification of Tomato Mottle Mosaic Virus and Tomato Brown Rugose Fruit Virus

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