An experiment was carried out at the Biotechnology Laboratory of Bangladesh Sugarcane Research Institute (BSRI), Ishurdi, Pabna for the development of drought tolerant somaclones. Five sugarcane varieties viz. Isd 20, Isd 35, Isd 36, Isd 37 and Isd 38 were used as plant material. Unexpanded spindle leaf sheaths were used as explants in tissue culture. In the first culture, the MS medium (BM) was supplemented with 2,4-D (3 mgL-1) and coconut water (10 %) for callus induction. The callus was then sub-cultured on fresh BM with BAP (2.0 mgL-1) and Kinetin (1.0 mgL-1) for plantlet initiation (2nd culture). In the third culture, initiated plantlets were sub-cultured again on fresh BM contained NAA (5.0 mgL-1) for root development. In all cultures BM was supplemented with 0.0, 5.0, 7.5 and 10.0% poly ethylene glycol (PEG) and was semi solidified with 0.6% agar to select somaclone variant plantlets of sugarcane in vitro. In the first culture 100% explants initiated callus on medium supplemented with no PEG. Callus induction, proliferation and plantlet regeneration decreased with increased level of PEG. At 7.5% PEG, the callus induction was highest (80%) in varieties Isd 35 and Isd 38. Callus was induced but became reddish black and senescence within 40 days on BM supplemented with 10.0 % PEG. Both shoot and root production decreased with increased PEG level in the medium. At 7.5 % PEG in BM, the highest shoot number was in Isd 38 (5.5 per culture), root number (7.6 per shoot) and root length (1.2 cm) were in the variety Isd 38. The highest shoot length was in Isd 37 and Isd 38 (1.8 cm). Survival percentage of in vitro regenerated plantlets was 100 % during hardening in low cost polythene house and in establishment in the field. Keywords: Somaclone; sugarcane; drought stress; plantlet; in vitro DOI: http://dx.doi.org/10.3329/agric.v9i1-2.9475 The Agriculturists 2011; 9(1&2): 18-28

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An investigation was conducted at two farmers' field viz Lalpur (Natore) and Sirajgonj during two consecutive cropping seasons (2003)(2004)(2005) to screen flood stress tolerant sugarcane clones. At Lalpur location clones such as I 110-98, I 139-98, I 142-98 and I 137-96 showed highly tolerant reaction having tolerance rating scale 1 against periodic natural flood stress, and obtained 118.8, 99.2, 108.3 and 121.4 t ha -1 estimated cane yield respectively. At Sirajgonj location clones such as I 82-98, I 98-98, I 110-98, I 139-98, I 142-98, I 202-97 and I 137-96 showed tolerant reaction having tolerance rating 2, and produced 114.3, 112.7, 112.3, 80.6, 102.8, 103.2 and 113.9 t ha -1 estimated cane yield respectively. Results indicate that BSRI bred above mentioned clones have built in potential to grow up to 90 cm depth periodic flood stress period for 90days.In Bangladesh flood stress tolerant variety development is a crying need for sustainable sugarcane cultivation since it is gradually pushed into low lying char areas prone to periodic inundation by flood water due to higher demand of cereal and vegetable crops.Sugarcane genotypes usually show different tolerance to high water tables based on shoot dry matter. Glaz et al. (2002) grew nine sugarcane cultivars under 15 and 38 cm water table depths. They reported a 8.3% mean yield reduction at 15 cm water table, but two cultivars showed similar yields at both water tables, used and yield of one cultivar was reduced by 25% at 15 cm water table. Deren and Raid (1997) tested four sugarcane cultivars for flood tolerance. Field was flooded for 10 after 3 days of planting. Over a 2-year period, yield reductions ranged from 12 to 16 % when flooded fields were compared to non-flooded fields. Information on yield and yield contributing characters under flood stress conditions are limited. Therefore, this study was undertaken to collect some information on yield potential of sugarcane genotypes under flood stress condition.The experiments were conducted at flood prone areas such as Lalpur (Natore) and Sirajgonj during 2003-2005 cropping seasons. Seven promising clones and one commercial variety (standard) were tested at both Lalpur (Natore) and Sirajgonj locations. The experiments were laid out in RCB design with three replications. Two budded setts were planted at trenches following end to end method of planting. NPKS fertilizers were applied @ 325 kg urea, 250 kg TSP, 190 kg MP, 180 kg Gypsum and 9 kg ZnSO 4 per hectare. Urea was applied in 3 splits and MP in two splits. Intercultural operations were done as and when required. The experimental field was inundated up to 90 cm by flood water, and lasted up to 90 days. Data on tolerance rating scale during flood stress period, while cane yield, tillers and millable canes production were recorded at harvest. Tolerance rating scale was recorded on the basis of greenness of leaves and other data recorded.The results on yield contributing characters and yield of flood affected clones have been presented in the Figs. 1 -2. In c...

The experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh from November 2021 to April 2022 to assess the effect of sulphur fertilization and weed management on yield performance of faba bean. The experiment consisted of three levels of sulphur viz. 0, 20 and 40 kg S ha-1 and four levels of weed management practices viz. control (weedy check), weed-free up to 30 days after sowing (DAS), weed-free up to 60 DAS, weed-free throughout the growth period. The experiment was laid out in a randomized complete block design with three replications.  The highest number of branches plant-1 (11.63), pods plant-1 (70.68), seeds pod-1 (5.50), 1000-seed weight (267.9 g), seed yield (2.72 t ha-1), and stover yield (2.93 t ha-1) were recorded with 40 kg ha-1.  In the case of weed management, the highest number of branches plant-1 (10.16), pods plant-1 (66.03), seeds pod-1 (6.0), 1000-seed weight (266.7 g), seed yield (2.43 t ha-1), stover yield (2.85 t ha-1) and harvest index (45.80%) was found at weed free throughout the growth period. Whereas the lowest values of all parameters were found minimum in weedy check. In interaction, the highest number of branches plant-1 (13.0), pods plant-1 (78.69), seeds pod-1 (7.0), 1000-seed weight (269.5 g), seed yield (2.87 t ha-1), stover yield (2.98 t ha-1) and harvest index (49.37%) were found in 40 kg ha-1 S with weed-free throughout the growth period. Whereas the lowest parameters were found minimum in control. Therefore, application 40 kg ha-1 S along with weed-free conditions throughout the growth period appears as the suitable combination for faba bean cultivation. Res. Agric. Livest. Fish. 10(1): 33-42.

The experiment was conducted during the period from November 2015 to June 2016 at the Laboratory of Biochemistry Department, Sher-e-Bangla Agricultural University (SAU) and Soil Science Laboratory of Bangladesh Agriculture Research Institute (BARI), to find out the effect of different wheat (Triticum aestivum L.) genotypes on proximate composition. Six wheat genotypes, i.e., BARI Gom 25, BARI Gom 26, BARI Gom 27, WYCYT (E-15) (Line), WYCYT (E-16) (Line) and PAVON-76 (Local) were used as a treatment for the experiment. The highest weight of 1000 grains, dry matter content, carbohydrate content, protein content, lipid content, ash content were recorded from BARI Gom 26 (46.45 g), BARI Gom 27 (92.53%), WYCYT (E-16) (69.59%), BARI Gom 27 (10.08%), BARI Gom 26 (2.20%), BARI Gom 26 (2.38%), respectively. Nevertheless, the lowest weight of 1000 grains, dry matter content, carbohydrate content, protein content, lipid content, ash content were recorded from PAVON-76 (Local) (39.25 g), WYCYT (E-15) (Line) (90.43%), BARI Gom 26 (64.87%), WYCYT (E-16) (Line) (9.5%), WYCYT (E-16) (Line) (1.74%), WYCYT (E- 16) (Line) (1.86%), respectively. SAARC J. Agric., 19(2): 171-179 (2021)