Microalgae is a very potential to be developed biota Because of its abundant amount on earth. Research on microalgae and its application as pigment analysis, provides important information about chemical compounds such as carotenoids and chlorophyll roomates that can be developed into drugs. Microalgae such as Chlorella vulgaris, Nannochloropsis sp., Porphyridium cruentum and Spirulina platensis has known had carotenoid pigments and chlorophyll content, but the optimization of the production of pigment in microalgae life phase has not been known in detail. Chlorophyll content in microalgae in the logarithmic phase of Chlorella vulgaris 200-1500 mg/L, Nannochloropsis sp. 100-500 g/L, Porphyridium cruentum 500-800 g/L and Spirulina platensis 1000-3500 mg/L and for stationary phases microalgae Chlorella vulgaris 100-1000 mg/L, Nannochloropsis sp. 200-500 g/L, Porphyridium cruentum 900-2000 mg/L and Spirulina platensis 2000-6000 mg/L. While the carotenoid content of microalgae in the logarithmic phase of Chlorella vulgaris 10-40 g/L, Nannochloropsis sp. 10-60 g/L, Porphyridium cruentum 10-60 ug/L and Spirulina platensis 20-40 ug/L and for stationary phases microalgae Chlorella vulgaris 10-50 g/L, Nannochloropsis sp. 10-70 g/L, Porphyridium cruentum 70-130 ug/L and Spirulina platensis 20-1100 mg/L.

Praktek Kerja Lapang (PKL) dilaksanakan di Unit Pelaksana Teknis Pengembangan Budidaya Air Tawar Umbulan, Kabupaten Pasuruan, Provinsi Jawa Timur pada tanggal 18 Desember 2017 – 18 Januari 2018. Tujuan dari Praktek Kerja Lapang ini adalah untuk mengetahui secara langsung serta mendapatkan pengalaman secara jelas tentang teknik pembenihan ikan mas (Cyprinus carpio) dalam pengembangan lebih lanjut dan permasalahan dalam usaha pembenihan ikan mas yang di hadapi serta cara pemecahannya. Metode yang di gunakan pada PKL ini adalah metode deskriptif, dengan teknik pengambilan data meliputi data primer dan data sekunder. Pengumpulan data ini dilakukan secara observasi, wawancara dan partisipasi aktif. UPT PBAT Umbulan memiliki luas keseluruhan lahan 6,25 hektar. Pemijahan ikan mas dilakukan secara alami. Induk ikan mas yang digunakan berasal dari Punten dan Sukabumi. Perbandingan berat induk ikan mas antaraojantan dan betina yaitu 2 : 1. Parameter Kualitas air untuk pembenihan ikan mas Antara lain : suhu 26,6-31,05 C; pH 7,1- 7,36; dan DO 6,23-10,8 mg/l. Berdasarkan hasil perhitungan, didapatkan nilai Fertilization Rate (FR) telur ikan mas sebesar 91,73%, Hatching Rate (HR) ikan mas sebesar 74,4% serta Survival Rate (SR) sebesar 30,44%. Pakan buatan yang digunakan dalam pemeliharan benih adalah pellet HI-PRO-VITE 781-3 yang telah dihaluskan dengan kadar protein 31%-33%. Benih ikan mas yang dipanen di UPT PBAT Umbulan memiliki ukuran 1-3 cm. UPT PBAT Umbulan mampu memproduksi benih sebanyak 50.000-100.000 ekor setiap bulan dengan harga benih diangka 10 Rp per benih ukuran 1-2 cm dan 50 Rp per benih ukuruan 5 cm.

S. costatum is one type of phytoplankton used in the maintenance of fish, shrimp, and crab larvae, but most are given in the maintenance of tiger shrimp larvae (Penaeus monodon) from the phase of nauplius phase after zoea. S. costatum has the advantages found in the autolysis enzyme itself so that it is easily digested by the larvae and does not pollute the cultivation media. Therefore knowledge about how is the effect of nutrients in S. costatum commercial life of food culture on laboratory scale up to mass scale. The method S. costatum is generally started by sterilizing tools and materials, preparation of culture media for laboratory scale (2 liters), semi-mass scale (80 liters) and mass scale (2 tons), fertilizer application, seed selection, the cost of counting S. costatum cells with peak population depends on the 36th hour with the highest number of cells in each culture scale items, namely laboratory scale of 602.547 × 103 cells/mL, semi-mass scale of 484.713 × 103 cells/mL and mass scale of 133.757 × 103 cells/mL then the last process is harvesting until it becomes flour. The obstacles encountered are environmental problems such as unstable weather and can be contaminated.

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The objectives of this research were: 1) determine the effect of fermented rice bran suspension concentration on the fecundity and production of M. macrocopa offspring per parent, 2) determine the optimal concentration of fermented rice bran suspension on the fecundity and production of M. macrocopa offspring per parent. This research used an experimental method with a completely randomized design consisting of six treatments of suspension of rice bran without fermentation (P0), 19.54 mg / L fermented rice bran suspension (P1), 21.98 mg / L fermented rice bran suspension (P2), 24.42 mg / L fermented rice bran suspension (P3), 26.68 mg / L fermented rice bran suspension (P4) and 29.30 mg / L fermented rice bran suspension (P5) in four replications for each. The results of the culture with a different concentration of rice bran suspension showed a significant difference (P <0.05) on the fecundity and production of M. macrocopa offspring per parent. The highest fecundity and production of M. macrocopa offspring per parent occurred in M. macrocopa culture with a concentration of fermented rice bran suspension of 29.30 mg / L (P5) with the fecundity 21-25 grains/parent and production of offspring 20-24 ind/parent.

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Land preparation in a traditional shrimp pond is one of the most important parts to do. Soil Redox Potential is an indispensable parameter to see the readiness of soil land or the level of fertility of cultivated land in carrying out activities. It is toxic to shrimp such as sulfide compounds (H2S), nitrites and ammonia. This research is descriptive in nature, by observing 5 traditional aquaculture ponds measuring 3500 m2, with shrimp densities of 100 individuals/m2. The use of Hydrogen Peroxide is pool 1 (0.5 ppm/m2), pool 2 (1 ppm/m2), pool 3 (1.5 ppm/m2), pool 4 (2 ppm/m2) and pool 5 as a control (0 ppm/m2). The basis for determining the use of Hydrogen Peroxide doses is from a minimum reference dose. The best results in pool 3 with a potential reduction value of 331 m/v, wherein one period the value of NH4 ranged from 0-2 ppm with ADG (Average Daily Growth) every week an average of 0.3 grams.

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