Artificial Insemination (AI) using sexed semen produce sex of calf as expected. There are various methods of sexed semen has been found. The aim of this research is to determine the effect of sexed semen methods using egg white sedimentation and percoll density gradient centrifugation toward motility and ratio of X and Y sperm Filial Ongole Cattle. Semen was collected using Artificial Vagina (AV) from Filial Ongole Cattle, evaluated and then separated using egg white sedimentation and percoll density gradient centrifugation technique. Only fresh semen with a minimum of 70% individual motile sperm and 2+ mass motility used in this study. Andromed as a based extender was diluted using aquabidest with 1:4 ratio. The obtained data were analyzed with analysis of variance (ANOVA) and continued by Duncan test if there was significant or very significant different. The result showed that the sexing methods (non sexing sperm, upper and under fraction using percoll density gradient centrifugation, upper and under fraction using egg white sedimentation technique) had very significant effect (P<0.01) on motility (64.25±3.94%; 48.55±8.28%; 53±7.93%; 56.9±8.22%; 49.75±8.19% respectively) and significant effect (P<0.05) on the ratio of X and Y sperm. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation methods for the upper fraction were 28.0±2.26%:72.0±2.26% and 77.5±1.26%:22.5±1.26%. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation technique for the under fraction were 69.0±15.35%: 26.0±4.37% and 22.9±1.44%: 77.1±1.44%. The study concludes that the sexed with egg white sedimentation technique is better than percoll density gradient centrifugation on sperm motility and ratio of X and Y sperm.
The objective of this study was to determine the effect of live weight (LW) on libido, sperm quality, testosterone, luteinizing hormone (LH) in Ongole grade (OG) bull. This study used 32 heads of OG bull with a completely randomized design that were divided to four different LW groups, i.e. group I was 170 to <210kg, group II was 210 to <250kg, group III was 250 to <290kg and group IV was 290 to 330kg. Parameters: observed were libido, testosterone hormone, LH, and semen quality. Data were analyzed by ANOVA. Libido of OG bull in group II and III higher than those in group IV. The testosterone hormone and LH showed that group II, III and IV were better than group I. The highest LH was in group IV, but low levels of testosterone were observed in these respective groups. Live sperm was the lowest in group I (21.25±13,20%) than II (66.00±11.17%), III (59.80±22.20%) and IV (75,67±9.46%). It can be concluded that the libido, sperm quality, testosterone, LH of OG bull in group II, III and IV were better than those in the group I, therefore standard LW of OG bulls had at least 250 kg.
<p class="abstrak2">The demand for meat in Indonesia cannot be supplied by domestic cattle production, and so breeder cattle stock are imported from abroad overseas to increase domestic production. A commonly imported breed is Brahman cross bred (Bx). Smallholder farmers have reported a wide range of problems associated with Bx cow reproductive performance. The purpose of this study was to identify changes in the reproductive performance of Bx cows by providing advice to farmers on improved management strategies, such as: maintaining body condition score (BCS) of cow> 3, appropriate estrous detection and weaning at 5- 6 months. This study was conducted from October 2011 – December 2013 by using 189 Bx cows and 151 farmers. The research was conducted in two sub districts of Lamongan district (East Java), Modo and Sambeng. Cattle were fed diets based on crop residues and were supplemented with green feeds, by-products (rice bran) and concentrate during the last 2 months of pregnancy and early lactation. The reproductive performance of cows improved during the study, there were a shorter lactation length/early weaning (reduced from 188 days to 153 days) and improved BCS (from 3.0 to 3.3). The length of postpartum anestrous interval (PPAI) decreased from 198 days to 98 days. Cycling to conception decreased from 75 days to 64 days and days open decreased from 217 days to 118 days over the length of the study. This was associated with improvement in BCS of cows at calving (from 3.0 to 3.3) and a shorter lactation length (reduced from 188 days to 153 days). There was no change in the average birth weight of calves (31.8 kg). There was no change in average weaning weight of calves over the year and average daily gain of calves while suckling 0.7 kg/day. The pre weaning calf mortality rate was decreased during this study (from 13% to 6%). This project demonstrated that Brahman cross cows can successfully be kept by smallholder farmers in Indonesia if appropiate management is used. It can be concluded that improving management, such as maintaining body condition score of cow >3, estrous detection, appropiate mating and weaning at 5-6 months can improve reproduction performance of Brahman cross.</p><p><strong>Key Words</strong>: Reproduction, Cattle, Brahman Cross</p>
ABSTRAK: Perkawinan sapi induk merupakan faktor penting dalam usaha perbibitan peternakan rakyat. Penelitian bertujuan untuk memperoleh waktu perkawinan sapi induk pasca beranak di kondisi peternakan rakyat. Penelitian dilakukan di Kabupaten Pasuruan dan Probolinggo Provinsi Jatim. Pengamatan dilakukan terhadap reproduksi dan produksi pada 280 sapi induk Peranakan Ongole (PO) selama tahun 2010-2013. Penelitian menggunakan rancangan acak kelompok dengan empat kelompok perlakuan waktu perkawinan sapi pasca beranak bebeda, yaitu Kelompok I (<60 hari), Kelompok II (60-<90 hari), Kelompok III (90-<120 hari), dan Kelompok IV (>120 hari); dengan jumlah masing-masing adalah 18, 57, 74 dan 59 ekor induk. Parameter: post partus anoestrus/PPAI, service per conception (S/C), conception rate (CR), waktu penyapihan pedet, dan calving interval/CI, persentase kelahiran dan kematian pedet. Data dianalisis menggunakan Anova; dilanjutkan dengan uji Duncan menggunakan SPSS. Nilai PPAI pada sapi induk PO menunjukkan perbedaan sangat nyata (P<0,01) antara kelompok waktu perkawinan; dengan PPAI pada kelompok I, II, III, dan IV masing-masing 44,7±6,2; 71,5±15,9; 92,1±23,0; dan 132,8±48,1 hari. Calving interval pada kelompok IV menunjukkan nilai tertinggi 499,0±123,4 hari (P<0,05) dibandingkan dengan dengan kelompok I, II, III. Nilai CR tertinggi dicapai pada kelompok III (79,45%) daripada CR I, II dan IV. Mortalitas pedet tertinggi dicapai pada kelompok I (16,67%) daripada kelompok II, III dan IV%. Persentase kelahiran pedet pada kelompok I, II, III dan IV adalah sama; namun waktu penyapihan pedet terendah diperoleh pada kelompok perkawinan II 92,8±22,5 hari (P<0,01) daripada kelompok I, III dan IV. Disimpulkan bahwa waktu perkawinan sapi induk yang efisien adalah 60-<90 hari pasca beranak. Kata kunci: Induk PO; pasca beranak; perkawinan; performans reproduksi.ABSTRACT: The mating of the beef cow is important factors in beef cattle breeding. The research aims to obtain the mating time of the cow after calving in smallholder farmers. The study was conducted on Pasuruan and Probolinggo Regency, East Java Province. Observations were on productivities performance in Ongole-cross breed cows in smallholder farmers from 2010 to 2013. The study used a randomized block design with four treatment groups postpartum mating, namely Group I (<60 days), Group II (60-<90 days), Group III (90-<120 days), and Group IV (>120 days. The parameters measured included postpartum anoestrus interval/PPAI, service per conception (S/C), conception rate (CR), calf weaning time, and calving interval/CI, percentage of calf birth and death. Data were analyzed using ANOVA; followed by the Duncan test using SPSS. The values of PPAI in PO cows showed very significant differences (P<0.01) between the mating time groups. Calving Interval in group IV showed the highest value of 499.0±123.4 days (P <0.05) compared with group I, II, III. The highest CR value was in group III (79.45%) than I, II and IV. The highest calf mortality was in the group I (16.67%) than in group II, I...
Excellent performance of bull as sperm producer was needed to maintain and increase the high pregnancy cows rate.The study aimed to determine the effect of mineral-vitamin combining [MVC] and herbs supplementing [HS]on feed intake, feed efficiency, average daily gain[ADG], linear body, semen quality and B/C ratio of OngoleCrossbred bull. Eight animals [aged 3 to 5 years] within initial weight 505.2 ± 70.5 kg were examined. They were grouped in two feed regimes, firstly, the basal diet was given with the inclusion of Vitamin A, E and Zinc-minerals [P1] and secondly, were basal diet plus herbs supplementation [P2]. The basal diet consisted of elephant grass, gliricidia, and commercial concentrates. Feeding was assigned to dry matter[DM] of 3% of body weight [BW] to meet the balance nutrient intake. The experimental which conducted as long as three months, was designed in two treatments and four replicates. Data analysed by using the T-test. There was no significant different between P1 and P2 in the results on feed intake, efficiency, ADG, and linear body. However, the sperm concentration of P1 [1,366.7 ± 768.9 million/ml] was higher[P<0.05] than those of P2 [873,3 ± 488.7 million/ml]. Meanwhile, the sperm viability of P1[90.4 ± 8.5%] was also higher than that of P2 [78.7 ± 16.2%]. Both P1 and P2 were recommended for being usedcommercially [due to requirement of Indonesia National Standard/SNI 4869-1:2017], but the P1 was the efficient one in regards of the B/C ratios.
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