Objetivo: Conhecer a situação da saúde mental dos profissionais da área da saúde da linha de frente na pandemia do COVID-19, e quais consequências para os serviços de saúde. Métodos: Trata-se de uma revisão integrativa de literatura e foi realizada nas bases de dados LILACS, MEDLINE pela biblioteca virtual da Saúde, na PUBMED e Scielo, usando descritores: Saúde mental / Mental health / Salud mental, Covid – 19 / Covid – 19 / Covid – 19, Profissional de saúde/ Health Personnel / Personal de Salud, Serviços de Saúde / Health Services / Servicios de Salud. Resultados: Foram selecionados 8 artigos para essa revisão. A partir da leitura dos artigos percebeu-se a fragilidade na saúde mental do trabalhador da saúde, dentro de incertezas do futuro e da cura do vírus COVID-19. Dessa forma, percebe-se altos índices de ansiedade, estresse, depressão, medo, angústia e sono alterado, doenças e muitas vezes esse profissional não procura ajuda para si mesmo. Considerações finais: O índice de sintomas psiquiátricos que já é alarmante normalmente no trabalhador da saúde, durante a pandemia tem se agravado. As organizações de Saúde precisam se atentar a isso e organizar maneiras de melhorar o bem-estar desses profissionais.

These findings substantiate the importance of using more than one gene protocol since the sensitivity and genetic variability changes with the locus used.Access numbers: The GenBank access numbers for the nucleotide sequences reported in this article are: JQ794877-JQ794890, JX033113-JX033118.

No abstract

Giardia duodenalis is a small intestinal protozoan parasite of several terrestrial vertebrates. This work aims to assess the genotypic variability of Giardia duodenalis isolates from cattle, sheep and pigs in the Southeast of Brazil, by comparing the standard characterization between glutamate dehydrogenase (gdh) and triose phosphate isomerase (tpi) primers. Fecal samples from the three groups of animals were analyzed using the zinc sulphate centrifugal flotation technique. Out of 59 positive samples, 30 were from cattle, 26 from sheep and 3 from pigs. Cyst pellets were stored and submitted to PCR and nested-PCR reactions with gdh and tpi primers. Fragment amplification of gdh and tpi genes was observed in 25 (42.4%) and 36 (61.0%) samples, respectively. Regarding the sequencing, 24 sequences were obtained with gdh and 20 with tpi. For both genes, there was a prevalence of E specific species assemblage, although some isolates have been identified as A and B, by the tpi sequencing. This has also shown a larger number of heterogeneous sequences, which have been attribute to mixed infections between assemblages B and E. The largest variability of inter-assemblage associated to the frequency of heterogeneity provided by tpi sequencing reinforces the polymorphic nature of this gene and makes it an excellent target for studies on molecular epidemiology.

Objetivo: Elaborar e validar um caso clínico para ser utilizado na simulação de uma situação realística do exame físico do sistema respiratório em um laboratório de práticas do Curso de Enfermagem. Métodos: Trata-se de estudo metodológico de construção e de validação de um caso clínico para ser utilizado em uma aula de simulação do exame físico do sistema respiratório. A caracterização da amostra de docentes e alunos que participaram na validação foi analisada de forma descritiva, sendo que os dados foram apresentados como média, mediana e desvio padrão O projeto foi aprovado pelo Comitê de Ética em Pesquisa da Universidade Federal de Uberlândia, sob o parecer nº 1.864.940. Resultados: Os avaliadores foram predominantemente do sexo feminino nos dois grupos. A idade foi diferente nos dois grupos (p = 0,008), sendo os docentes apresentaram idade mediana superior aos alunos (38 versus 23 anos). Quanto aos itens da validação do caso clínico, nenhum apresentou diferença significativa entre os dois grupos, docentes versus discentes (p > 0,05). Todos os itens receberam avaliação mediana superior a 2,75 e média superior a 2,48. Conclusão: O caso clínico foi elaborado e validado pelos enfermeiros especialistas e pelos alunos do Curso de Graduação em Enfermagem.

The new pneumonia caused by coronavirus 2 of severe acute respiratory syndrome (COVID-19) has the potential to develop biopsychosocial instability in the population and is responsible for much of the increase in mental disorders that occur after the beginning of the pandemic, especially among health professionals working on the front line. Physical exhaustion and mental distress leads them to search alternative therapies for harm reduction, such as therapeutic communication therapies, active listening, meditation, mindfulness and yoga. Such actions can decrease stress and have potential harm reduction in relation to the development of posttraumatic stress disorder. In addition, religiosity and/or spirituality reduced the psychological suffering of health workers, not only in moments of pandemic, but in the daily work routines.

No abstract

The COVID-19 pandemic has transformed the reality, made social isolation urgent aiming at reducing the contagion of the disease and, as a consequence, had to interrupt the classes and actions of extension projects in person. In this aspect, the Internet has become a possibility of interaction between teachers, nursing students and the community in an extension project university entitled: "Aromatherapy as a tool for coping in times of pandemic. Thus, the present study is a report of the authors' experience in the project of  aromatherapy as an activity for health promotion in times of pandemic by COVID-19. A descriptive qualitative report was carried out on the experiences of the authors with the use of a virtual platform as an alternative informative content on the subject, during this period. The project was carried out in five weeks, with three synchronous meetings, the programmatic content was guided by scientific evidence, with guidelines of great importance to the public assisted with the aim of promoting health and be a tool for coping in times of pandemic. Thus, access to health promotion and exchange of knowledge of the target audience of the extension project was expanded.