The objective of this study was to evaluate the influence of five levels of N and two regrowth intervals on the structural, productive, and nutritional characteristics of Tifton 85 grass (Cynodon spp). The experiment was conducted in a hay production field at the State University of West Paraná, planted in 2004 with Cynodon spp. cv. Tifton 85. The experiment was established in a randomized block design with factorial treatments of five N fertilization amounts (0, 25, 50, 75 and 100 kg ha -1 cut -1 ) applied as urea after each cut, and two regrowth intervals (28 and 35 d), with four replicates. N application promoted changes in plant structure, such as increased canopy height, stem length, leaf elongation rate, and dry mass (DM) production. The greatest N use efficiency, considering the four harvests, occurred in the plot with 25 kg N ha -1 cut -1 at the 35-d interval, with a production of 13.79 kg DM kg -1 of N applied. A nutritional analysis indicated that 100 kg ha -1 of N cut -1 promoted higher crude protein (CP) at 28 d, with the highest concentration observed in the fourth cut (208.2 g kg -1 DM) and the lowest in the second cut (140.12 g kg -1 DM). The content average in the four cuts for CP and LIG at 28 days of regrowth was 175.85 and 104.33 kg -1 g MS and at 35 days of regrowth interval was of 164.45 and 118.65 g kg -1 DM, respectively. No differences were found between regrowth intervals in the contents of mineral matter and acid detergent fiber (ADF). Environmental factors including the wide variation in precipitation (greater than 200 mm between the peak in December 2010 and the lowest in March 2011) greatly influenced Tifton 85 grass production, affecting DM and the nutritional value of the forage in each cut. Key words: Biomass. Forage quality. Grazing. Management. Persistence. Stocking. ResumoO objetivo do trabalho foi avaliar a influência de cinco doses de nitrogênio (N) em duas idades de rebrota no capim Tifton 85 (Cynodon spp.) sobre as características estruturais, produtivas e na composição bromatológica. O experimento foi instalado no campo de produção de feno da UNIOESTE, campus de Marechal Cândido Rondon. O delineamento experimental foi em blocos casualizados em esquema fatorial 5x2, sendo cinco doses de nitrogênio (0, 25, 50, 75 e 100 kg ha -1 ) aplicados em cada corte, aplicadas na forma de uréia, duas idades de rebrota (28 e 35 dias) com quatro repetições e quatro cortes.

RESUMOEste trabalho teve por objetivo avaliar o efeito dos sistemas de ordenha e de resfriamento na contagem bacteriana total (CBT) e na quantidade de leite com CBT ≤ 10 5 , que representa leite de alta qualidade. Um total de 12.221 registros de CBT do leite foi obtido de uma indústria láctea da região Oeste do Paraná, no período de janeiro a outubro de 2006. Após as coletas, a qualidade do leite foi analisada em laboratório pelo método de citometria de fluxo. Os dados de CBT foram classificados por sistema de ordenha, tipo de resfriador e intervalos da CBT, segundo critério da Instrução Normativa 62, e submetidos à análise de independência de Qui-Quadrado. As proporções de CBT do leite entre tipos de resfriadores, em cada sistema de ordenha, foram comparadas utilizando-se a estatística calculada Z. Observou-se menor (p < 0,01) valor de CBT do leite de vacas submetidas à ordenha canalizada e do leite resfriado a granel. A proporção das amostras de leite avaliadas nos três níveis de CBT resfriadas por sistema de imersão não diferiu (p = 0,1549) do leite resfriado a granel, quando o leite foi obtido de rebanhos ordenhados por ordenha canalizada. A alta qualidade microbiológica do leite da região Oeste do Paraná é obtida quando os produtores utilizam em suas propriedades rurais a ordenha canalizada e o sistema de resfriamento a granel. PALAVRAS-CHAVE: Higienização, ordenhadeira, qualidade microbiológica, resfriador. ABSTRACT TOTAL BACTERIAL COUNT OF MILK IN DIFFERENT SYSTEMS OF MILKING ANDCOOLING. This study aimed to evaluate the effect of milking systems and cooling on total bacterial count (TBC), and the amount of milk samples with TBC ≤ 10 5 , which represents high-quality milk. A total of 12,221 records of milk TBC were obtained from a dairy industry in the western region of Paraná State, Brazil, from January to October 2006. After the sampling was conducted, the milk quality was analyzed in the laboratory by the method of flow cytometry. The TBC data were classified by milking system, type of coolers and intervals of the TBC, in accordance with Normative Instruction 62, and submitted to the chi-square test for independence. The proportions of milk TBC among types of coolers at each milking system were compared using the calculated statistic Z. A lower TBC value (p < 0.01) was observed in milk obtained from cows milked using a piped system and from bulk cooled milk. The proportion of milk samples evaluated at three levels of TBC cooled by the immersion system did not differ (p = 0.1549) from bulk cooled milk when the milk was obtained from herds milked with a piped system. The best microbiological quality of milk in the western region of Paraná was achieved with a piped milking system and bulk cooling.KEY WORDS: Cleaning, cooler, microbiological quality, milking. INTRODUÇÃOO parâmetro utilizado com maior frequência para avaliar a qualidade do leite é a contagem bacteriana total (CBT) (Bava et al., 2009). O valor da CBT acima dos limites tolerados pela legislação é indicativo de deficiência na limpeza e higieniz...

ResumoObjetivou-se verificar o efeito de três volumes mensais de venda de leite, meses do ano, tipos de resfriadores e sistemas de ordenha sobre o ponto de congelamento do leite, o percentual de gordura, a contagem de células somáticas (CCS) e a contagem bacteriana total (CBT) do leite oriundo de produtores de um laticínio do Oeste do Paraná. Avaliou-se doze meses para cada informação (volume, gordura, crioscopia, CCS e CBT) com um total de 7.105 registros. As amostras para as análises foram coletadas pelos transportadores de leite junto aos produtores. As informações foram classificadas pelos volumes mensais produzidos (até 4.500 litros, entre 4.500-15.000 litros e mais que 15.000 litros), pelos sistemas de ordenha (manual, balde ao pé, canalizado e com transferidor) e tipos de resfriadores (imersão, granel e freezer) utilizados por cada produtor. O leite oriundo de produtores com volumes superiores a quinze mil litros mensais possui menor ponto crioscópico, teor de gordura, CBT e CCS. Ocorreram variações estacionais do ponto de congelamento, teor de gordura, CBT e CCS. As variações da CBT durante os períodos do ano são menores no leite oriundo de resfriamento a granel e de ordenhadeiras canalizadas. Existem fatores relacionados ao manejo dos animais e higienização dos equipamentos de ordenha e resfriamento que necessitam ser trabalhados junto aos produtores. Palavras-chave: Crioscopia, contagem bacteriana total, contagem de células somáticas, gordura AbstractAimed to evaluate the effect of three volumes monthly milk sales, months of year, types of coolers and milking systems on the freezing point of milk, fat percentage, somatic cell count (SCC) and bacterial count Total (CBT) milk come from producers that sell to a dairy of Western of Paraná. We evaluated twelve months for volume, fat, freezing point, SCC and TBC with a total of 7,105 records. Samples for analysis were collected by milk transporters. The information was classified by monthly volume purchased from each producer (up to 4,500 gallons between 4,500-15,000 liters and more than 15,000 liters), by milking systems (manual, bucket foot, piped and protractor) and types of coolers (immersion 1 Médico Veterinário, M.e em Zootecnia, Dr. em Agronomia, Universidade Estadual do Oeste do Paraná, UNIOESTE, Marechal Cândido Rondon, PR.

-The objective of this study was to evaluate the microbiological profile and aerobic stability of silage with Tifton 85 bermudagrass and different additives and wilting. The studied treatments were: pre-drying in the sun for two hours before ensiling; use of bacterial-enzymatic inoculant; addition of soybean peel; addition of corn grits and use of salt in the surface layer of the silo. The experimental design was completely randomized with six treatments and four replications. Plants of Tifton 85 bermudagrass with 38 days were ensiled in experimental silos with Bunsen valves, with compacting density of 236 kg silage per m 3 for Tifton 85 bermudagrass, which was pre-dried in the sun; the average silage was 294 kg m -3 for the other treatments. The ratios between soybean hulls and corn grits, added to the silage, were calculated based on the initial DM content from Tifton 85 bermudagrass in order to obtain 320 g/kg DM of the material to be ensiled. There was an increase of lactic bacteria and Clostridium as well as an absence of enterobacteria after the silo opening. Fungi developed only in Tifton 85 bermudagrass and its treatments before the ensilage and yeasts developed in silages of Tifton 85 bermudagrass when they received soybean hulls or corn grits after silo opening. There was no breach regarding aerobic stability of silages during the studied period. The pH reached the highest values within 48 hours after opening the silos, but there were variations during this period.

Objetivou-se avaliar as etapas de produção de feno de capim Tifton 85 adubado com cinco doses de nitrogênio após cada corte sob a forma de ureia em cobertura. A desidratação da primeira colheita ocorreu durante seis dias em galpão, em função das condições climáticas, e a desidratação da segunda colheita ocorreu a campo durante dois dias. O delineamento experimental foi em blocos ao acaso com parcelas subdivididas no tempo, sendo as parcelas (5m x 3m) as doses de nitrogênio (0; 25; 50; 75 e 100kg ha-1 colheita-1) e as subparcelas as etapas de produção de feno (corte, enfardamento e armazenamento). O nitrogênio aumentou a altura e produção de matéria seca por hectare, os teores de proteína bruta (PB) e de proteína insolúvel em detergente ácido (PIDA) e neutro (PIDN). A secagem em galpão durante seis dias foi insuficiente para que a forragem atingisse 850g kg-1 de MS no momento de enfardar. Entre a etapa de corte e 30 dias de armazenamento, o feno desidratado em galpão aumentou os teores de fibra em detergente neutro, de fibra em detergente de ácido, a PIDA, PIDN e a lignina e ocorreu um decréscimo de 18,9% nos teores de PB. Ocorreu redução da digestibilidade in vitro da matéria seca (DIVMS) em 32,72% entre a etapa de corte e 30 dias de armazenamento para o feno seco em galpão, enquanto que não houve alteração significativa na DIVMS e PB para o feno seco em dois dias a campo.

The objective of this study was to evaluate Cynodon species 'Tifton 85' at different hay production phases (harvest, baling, and storage) and different (N) rates. Dehydration of the first crop was carried out in the field, while the second crop was in the shed. Dry matter and protein content increased linearly with the N rates. Dehydration in the shed was not effective for hay production, since dry matter did not reach the desired level of 850 g kg -1. During the hay production period (harvest, baling, and storage), the content of acid detergent fiber of the first crop and the second crop increased by 25.32 and 7.38%, respectively, and that of lignin increased by 21.33 and 32.27%, respectively. Forage digestibility decreased by 4.55%, when dehydration occurred in the field, whereas it decreased by 14.68% when dehydration occurred in the shed, a difference higher than 300% due to the loss of soluble carbohydrates. Overall, the findings of this study indicate that forage dehydration for hay production needs to be carried out in the field under appropriate environmental conditions to prevent nutritional losses. Additionally, dry matter of Tifton 85 increased by 20.40% and protein content increased by 18.65%, which equals 514.27 kg of soybean meal at 100 kg ha -1 of N.

Some winter crops sown in no-tillage system can represent an important alternative to nutrient cycling. The objective of this work was to evaluate the production of dry matter (DM) and accumulation of nutrients for winter cultivation in the West of Paraná. The experimental design was a randomized block, with four treatments and six replications. The treatments were represented by four different winter crops (oat IPR 126, crambe FMS Brilhante, radish common cultivar and wheat BRS Taruma), and the DM, the contents of C, N, P, K, Ca, Mg and C/N ratio in DM and nutrients accumulation were determied The dry matter production was higher for radish with 4.929,14 kg ha -1 . The different winter crops used influenced the contents of C, N and C/N ratio. The other studied characteristics were not influenced. Among the four winter cultivation the radish presented larger production of dry matter. The chemical composition was influenced by the cultivations, the contents of C, N and C/N ratio, consequently in the contribution differentiated in the area. The winter cultivation in the studied conditions influences the accumulation of magnesium.

Morphological characteristics, dry matter production, and nutritional values of winter forage and grains were evaluated. This study was conducted from April 24, 2012 to November 7, 2013 in the Western Paraná State University (UNIOESTE), Marechal Cândido Rondon, Brazil. Pastures under one grazing and non-grazing conditions were evaluated under 120 kg N ha-1 fertilization split into two 60 kg N ha-1 treatments. Two pastures received 40 kg N ha-1 three times. IPR 126 oat, BRS Tarumã wheat, and IPR 111 triticale were the test crops. Topdressing with 40 or 60 kg N ha-1 did not change morphological characteristics until 60 d after sowing. Pastures under non-grazing that received 120 kg N ha-1 treatments were taller than the controls, whereas those under grazing that received 80 or 120 kg N ha-1 presented with higher leaf production than did the controls. Total average dry matter (DM) production in 2012 and 2013 was, respectively, 5,275 kg ha-1 and 6,270 kg ha-1 for oat, 3,166 kg ha-1 and 7,423 kg ha-1 for wheat, and 4,552 kg ha-1 and 7,603 kg ha-1 for triticale. Split N fertilization did not cause differences in the levels of crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) in the forage. Nevertheless, increases in in vitro dry matter digestibility (IVDMD) were observed in oat and wheat receiving 60 kg N ha-1 during the first graze. IVDMD did not change in oat, wheat, and triticale forages receiving 80 or 120 kg N ha-1 during the second graze. Grazing did not affect the nutritional values of wheat and triticale grains, but reduced those of oat. Therefore, the results of the present study suggest that grazing lengthens the crop cycles, and so allow the staggered sowing of summer crops.