RESUMO:Os jovens cada vez mais cedo planejam o curso que pretendem ingressar após o ensino médio, para isso, a pesquisa objetivou conhecer as preferências dos discentes de escolas públicas localizadas na cidade de Capistrano/CE quanto ao curso superior que pretendem ingressar, bem como a atratividade da carreira docente entre esses alunos. Para tanto, aplicouse um questionário a 228 concluintes do ensino médio durante o primeiro semestre de 2016. Obteve-se que os cursos de licenciatura foram predominantes na escolha, apesar do pouco incentivo por parte dos pais e familiares para ingressarem na carreira docente e de reconhecerem as dificuldades que a profissão enfrenta hoje no país. Palavras-chave: Curso superior. Ensino Médio. Docência. Carreira profissional.ABSTRACT: Young people are planning the course that they intend to attend after high school, so the research aimed to know the preferences of the students of public schools located in the city of Capistrano/CE regarding the course they intend to enter, as well as attractiveness Of the teaching career among these students. To that end, a questionnaire was applied to 228 high school students during the first semester of 2016. It was concluded that degree's courses were predominant in the choice, despite the lack of encouragement from parents and family members to enter the teaching profession and To recognize the difficulties that the profession faces today in the country.
Persister cells are metabolically inactive dormant cells that lie within microbial biofilms. They are phenotypic variants highly tolerant to antimicrobials and, therefore, associated with recalcitrant infections. In the present study, we investigated if Trichosporon asahii and T. inkin are able to produce persister cells. Trichosporon spp. are ubiquitous fungi, commonly found as commensals of the human skin and gut microbiota, and have been increasingly reported as agents of fungemia in immunocompromised patients. Biofilms derived from clinical strains of T asahii (n=5) and T. inkin (n=7) were formed in flat-bottomed microtiter plates and incubated at 35°C for 48 h, treated with 100 μg/ml amphotericin B (AMB) and incubated at 35°C for additional 24 h. Biofilms were scraped from the wells and persister cells were assayed for susceptibility to AMB. Additionally, we investigated if these persister cells were able to generate new biofilms and studied their ultrastructure and AMB susceptibility. Persister cells were detected in both T asahii and T. inkin biofilms and showed tolerance to high doses of AMB (up to 256 times higher than the minimum inhibitory concentration). Persister cells were able to generate biofilms, however they presented reduced biomass and metabolic activity, and reduced tolerance to AMB, in comparison to biofilm growth control. The present study describes the occurrence of persister cells in Trichosporon spp. and suggests their role in the reduced AMB susceptibility of T. asahii and T. inkin biofilms.
Trichosporon species have been considered important agents of opportunistic systemic infections, mainly among immunocompromised patients. Infections by Trichosporon spp. are generally associated with biofilm formation in invasive medical devices. These communities are resistant to therapeutic antifungals, and therefore the search for anti-biofilm molecules is necessary. This study evaluated the inhibitory effect of farnesol against planktonic and sessile cells of clinical Trichosporon asahii (n = 3) andTrichosporon inkin (n = 7) strains. Biofilms were evaluated during adhesion, development stages and after maturation for metabolic activity, biomass and protease activity, as well as regarding morphology and ultrastructure by optical microscopy, confocal laser scanning microscopy, and scanning electron microscopy. Farnesol inhibited Trichosporon planktonic growth by 80% at concentrations ranging from 600 to 1200 μM for T. asahii and from 75 to 600 μM for T. inkin. Farnesol was able to reduce cell adhesion by 80% at 300 μM for T. asahii and T. inkin at 600 μM, while biofilm development of both species was inhibited by 80% at concentration of 150 μM, altering their structure. After biofilm maturation, farnesol decreased T. asahii biofilm formation by 50% at 600 μM concentration and T. inkin formation at 300 μM. Farnesol inhibited gradual filamentation in a concentration range between 600 and 1200 μM. Farnesol caused reduction of filament structures of Trichosporon spp. at every stage of biofilm development analyzed. These data show the potential of farnesol as an anti-biofilm molecule.
Este estudo teve por objetivo investigar o processo educativo ambiental em uma escola pública de Ensino Fundamental no município de Capistrano-CE. Trata-se de pesquisa analítica de abordagem quantitativa, realizada com 40 alunos que responderam a um questionário contendo perguntas sobre conceitos e práticas de educação ambiental. Os resultados reforçam que a prática ambiental na escola e aulas diferenciadas são fundamentais para a consolidação de atitudes socioambientais e que a escola, como promotora de conhecimento e cidadania, algumas vezes esquiva-se não atendendo aos anseios socioambientais de seus alunos. Concepts, practices of environmental education and citizen training in school The objective of this study was to investigate the environmental education process in a public elementary school in the municipality of Capistrano-CE. This is an analytical research with a quantitative approach, carried out with 40 students from a municipal public school. A questionnaire containing questions that addressed ambiental education (EA) concepts and practices was applied to the participants. The results indicate that the teaching and practice of EA in schools are important contributors to social practices, being the environmental practice in school and differentiated classes as fundamental to social and environmental practices. It is, therefore, evidenced that the school, as promoter of knowledge and citizenship, sometimes dodges, failing to meet the socioenvironmental desires of its students. Keywords: Environmental Education. educational process. environmental practice.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.