The production rates and yields were investigated at 30 °C in a 2.3 l bioreactor operated in 19 batch and sequenced-batch mode using glucose and starch as substrates. In order to study the 20 precise effect of a stable pH on hydrogen production, and the metabolite pathway involved, 21 cultures were conducted with pH controlled at different levels ranging from 4.7 to 7.3 22 (maximum range of 0.15 pH unit around the pH level). For glucose the maximum yield (1.7 23 mol H 2 mol -1 glucose) was measured when the pH was maintained at 5.

12This paper investigated the enhancement effect of nanometre-sized metallic (Pd, Ag and Cu)

In this paper, a simple and rapid method was developed in order to assess in comparative tests the production of binary biogas mixtures containing CO 2 and another gaseous compound such as hydrogen or methane. This method was validated and experimented for the characterisation of the biochemical hydrogen potential of different pure strains and mixed cultures of hydrogen-producing bacteria (HPB) growing on glucose.The experimental results compared the hydrogen production yield of 19 different pure strains and sludges : facultative and strict anaerobic HPB strains along with anaerobic digester sludges thermally pre-treated or not. Significant yields variations were recorded even between different strains of the same species by i.e. about 20% for three Clostridium butyricum strains. The pure Clostridium butyricum and pasteurianum strains achieved the highest yields i.e. up to 1,36 mol H 2 /mol glucose compared to the yields achieved by the sludges and the tested Escherichia and Citrobacter strains.

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