Optimization of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode

Hamilton, Christopher; Hiligsmann, Serge; Beckers, Laurent; Masset, Julien; Wilmotte, Annick; Thonart, Philippe

doi:10.1016/j.ijhydene.2009.10.073

Cited by 38 publications

(22 citation statements)

References 46 publications

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“…The HPLC analysis was carried out using an Agilent 1110 series (HP Chemstation software) with a Supelcogel C-610H column preceded by a Supelguard H pre-column (oven temperature 40°C), 0.1 % H 3 PO 4 (in milliQ water) as the isocratic mobile phase at a flow rate of 0.5 ml.min _1 and a differential refraction index detector (RID, heated at 35°C). The process lasted for 35 min at a maximum pressure of 60 bars as described formerly (Hamilton et al 2010).…”

Section: Methodsmentioning

confidence: 99%

Fermentation profile of Saccharomyces cerevisiae and Candida tropicalis as starter cultures on barley malt medium

et al. 2014

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Saccharomyces cerevisiae C8-5 and Candida tropicalis F0-5 isolated from traditional sorghum beer were tested for kinetic parameters on barley malt extract, YPD (863 medium) and for alcohol production. The results showed that C. tropicalis has the highest maximum growth rate and the lowest doubling time. Values were 0.22 and 0.32 h −1 for maximum growth rate, 3 h 09 min and 2 h 09 min for doubling time respectively on barley malt extract and YPD. On contrary, glucose consumption was the fastest with S. cerevisiae (−0.36 and −0.722 g/l/h respectively on barley malt extract and YPD). When these two yeasts were used as starters in pure culture and co-culture at proportion of 1:1 and 2:1 (cell/cell) for barley malt extract fermentation, we noticed that maltose content increased first from 12.12 g/l to 13.62-16.46 g/l and then decreased. The highest increase was obtained with starter C. tropicalis + S. cerevisiae 2:1. On contrary, glucose content decreased throughout all the fermentation process. For all the starters used, the major part of the ethanol was produced at 16 h of fermentation. Values obtained in the final beers were 11.4, 11.6, 10.4 and 10.9 g/l for fermentation conducted with S. cerevisiae, C. tropicalis, C. tropicalis + S. cerevisiae 1:1 and C. tropicalis + S. cerevisiae 2:1. Cell viability measurement during the fermentation by using flow cytometry revealed that the lowest mean channel fluorescence for FL3 (yeast rate of death) was obtained with C. tropicalis + S. cerevisiae 2:1 after 48 h of fermentation.

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Section: Methodsmentioning

confidence: 99%

Fermentation profile of Saccharomyces cerevisiae and Candida tropicalis as starter cultures on barley malt medium

et al. 2014

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“…on-column injections of 50 µL gas samples on CarboPLOT P7 column (Varian Chrompack, NL) in a gas chromatography system (HP8950 SeriesII) equipped with a TCD detector, using either nitrogen or helium as carrier gas (respectively for hydrogen and for nitrogen/methane/carbon dioxide detection). A detailed procedure can be found elsewhere [37].…”

Section: Hydrogen Production Measurementmentioning

confidence: 99%

Investigation of the links between mass transfer conditions, dissolved hydrogen concentration and biohydrogen production by the pure strain Clostridium butyricum CWBI1009

Beckers

Masset

Hamilton

et al. 2015

Biochemical Engineering Journal

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Fermentative hydrogen production has often been described as inhibited by its own gas production. In this work, hydrogen production by Clostridium butyricum was investigated in batch Biochemical Hydrogen Potential (BHP) tests and in a 2.5 L Anaerobic Sequenced Batch Reactor (AnSBR) under different operating conditions regarding liquid-to-gas mass transfer.Through the addition of both stirring up to 400 RPM and nitrogen sparging, the yields were enhanced from 1.6 to 3.1 mol H2 ·mol glucose -1 and the maximum hydrogen production rates from 140 to 278 mL·h -1 . These original results were achieved with a pure Clostridium strain. They showed that hydrogen production was improved by a higher liquid-to-gas hydrogen transfer resulting in a lower dissolved hydrogen concentration in the culture medium and therefore in a lower bacterial inhibition. In addition, biohydrogen partitioning between the gas and the liquid phase did not conform to Henry's Law due to critical supersaturation phenomena up to seven-fold higher than the equilibrium conditions. Therefore dissolved hydrogen concentration should be systematically measured instead of the headspace hydrogen partial pressure. A model was proposed to correlate H 2 production yield and rate by the pure C.butyricum strain CWBI1009 with mass transfer coefficient K L a.

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“…Gas chromatography and high performance liquid chromatography analysis H 2 , CH 4 , and CO 2 were determined using a method described by Hamilton et al (2010), and separation was achieved using a Hewlett Packard 5890 Series II gas chromatograph (GC; Agilent Technologies, Santa Clara, CA, USA) equipped with a 30 m long, 0.32 mm id Alltech GAS PRO GSC column (Grace, Deerfield, IL, USA) in series with a 20 m long, 0.25 mm id…”

Section: Koh Operating Proceduresmentioning

confidence: 99%

Comparative biochemical analysis during the anaerobic digestion of lignocellulosic biomass from six morphological parts of Williams Cavendish banana (Triploid Musa AAA group) plants

Kamdem

Hiligsmann

Vanderghem

et al. 2013

World J Microbiol Biotechnol

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Significance statement: In this original paper, we present the biochemical composition and potential methane production from the anaerobic digestion of each type of lignocellulosic waste from a banana cultivar (Williams Cavendish: triploid Musa AAA group). These wastes are usually abandoned in the plantation after the fruits have been harvested. There is great interest in obtaining energy from this generally neglected biomaterial, particularly in the contexts of global warming and sustainable development. 2Abstract: We studied banana lignocellulosic biomass (BALICEBIOM) that is abandoned after fruit harvesting, and assessed its biochemical methane potential, because of its potential as an energy source. We monitored biogas production from six morphological parts (MPs) of the "Williams Cavendish" banana cultivar using a modified operating procedure (KOP) using KOH. Volatile fatty acid (VFA) production was measured using high performance liquid chromatography. The bulbs, leaf sheaths, petioles-midribs, leaf blades, rachis stems, and floral stalks gave total biogas production of 256, 205, 198, 126, 253, and 221 mL g −1 dry matter, respectively, and total biomethane production of 150, 141, 127, 98, 162, and 144 mL g −1 , respectively. The biogas production rates and yields depended on the biochemical composition of the BALICEBIOM and the ability of anaerobic microbes to access fermentable substrates. There were no significant differences between the biogas analysis results produced using KOP and gas chromatography. Acetate was the major VFA in all the MP sample culture media.The bioconversion yields for each MP were below 50%, showing that these substrates were not fully biodegraded after 188 d.The estimated electricity that could be produced from biogas combustion after fermenting all of the BALICEBIOM produced annually by the Cameroon Development Corporation-Del Monte plantations for 188 d is approximately 10.5×10 6 kW h (which would be worth 0.80-1.58 million euros in the current market). This bioenergy could serve the requirements of about 42000 people in the region, although CH 4 productivity could be improved.

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Optimization of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode

Cited by 38 publications

References 46 publications

Fermentation profile of Saccharomyces cerevisiae and Candida tropicalis as starter cultures on barley malt medium

Fermentation profile of Saccharomyces cerevisiae and Candida tropicalis as starter cultures on barley malt medium

Investigation of the links between mass transfer conditions, dissolved hydrogen concentration and biohydrogen production by the pure strain Clostridium butyricum CWBI1009

Comparative biochemical analysis during the anaerobic digestion of lignocellulosic biomass from six morphological parts of Williams Cavendish banana (Triploid Musa AAA group) plants

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