Comparative study of biological hydrogen production by pure strains and consortia of facultative and strict anaerobic bacteria

Abstract

In this paper, a simple and rapid method was developed in order to assess in comparative tests the production of binary biogas mixtures containing CO 2 and another gaseous compound such as hydrogen or methane. This method was validated and experimented for the characterisation of the biochemical hydrogen potential of different pure strains and mixed cultures of hydrogen-producing bacteria (HPB) growing on glucose.The experimental results compared the hydrogen production yield of 19 different pure strains and sludges : facultative and strict anaerobic HPB strains along with anaerobic digester sludges thermally pre-treated or not. Significant yields variations were recorded even between different strains of the same species by i.e. about 20% for three Clostridium butyricum strains. The pure Clostridium butyricum and pasteurianum strains achieved the highest yields i.e. up to 1,36 mol H 2 /mol glucose compared to the yields achieved by the sludges and the tested Escherichia and Citrobacter strains.

Investigation of the links between mass transfer conditions, dissolved hydrogen concentration and biohydrogen production by the pure strain Clostridium butyricum CWBI1009

“…The BHP experiments were carried out for 96 hours in 270 mL serum bottles with 200 mL of liquid medium according to the method reported by Hiligsmann et al [35]. Four conditions were investigated in independent triplicate experiments: vertical non-stirred (VNS) and horizontal non stirred (HNS) bottles, developing respectively gas-liquid interfacial area of 28 cm² and 63.5 cm² respectively; vertical stirred bottles (VS) with orbital shaking at 120 rpm, and horizontal stirred bottles (HS), filled with reticulated polyurethane cubes (1.5 cm x 1,5 cm size; specific surface area +/-1800m²/m³, Type Filtren TM30, Recticel, Belgium) as a biomass carrier and rolled on their horizontal axis at 20 rpm.…”

Comparative biochemical analysis during the anaerobic digestion of lignocellulosic biomass from six morphological parts of Williams Cavendish banana (Triploid Musa AAA group) plants

“…The pH was adjusted with a 0.5 N KOH solution to achieve an initial pH of 7.3 in each sample, and a maximum variation during the culture period of pH 0.15 was maintained. The sample bottles were capped tightly with rubber septa and sealed with aluminum seals, and nitrogen was passed into the bottles to flush out air and other gases before the incubation (Hiligsmann et al 2011). The bottles were then incubated at 30 °C and monitored for 188 d.…”

“…Water containing 9 N KOH in 100 mL gas replacement equipment was used to monitor the biogas production and composition, and the absorption potential of the KOH solution was regularly measured using gas mixtures containing 0, 20, 35, 80, and 100% CO 2 (Hiligsmann et al 2011). The H 2 (for the first 8 d of fermentation), CH 4 , and CO 2 contents were determined for each gas sample using the procedure published by Hiligsmann et al (2011) but adapted for the biomethanation of BALICEBIOM.…”

“…The H 2 (for the first 8 d of fermentation), CH 4 , and CO 2 contents were determined for each gas sample using the procedure published by Hiligsmann et al (2011) but adapted for the biomethanation of BALICEBIOM.…”

Fermentative Hydrogen Production by Molasses; Effect of Hydraulic Retention Time, Organic Loading Rate and Microbial Dynamics

“…Acetate and butyrate are typical for H2 production by mixed acid fermentation of sucrose as already mentioned in introduction. Lactate can be produced by either lactic acid bacteria or by bacteria of the genus Clostridium via a pathway that does not promote H2 production (Hiligsmann et al, 2011).…”