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The aim of study was access the cobalt and lead contamination in soil, forages and animals. Heavy metal pollution is a matter of prime significance in natural environment. Through food chain toxicity of heavy metals and their bioaccumulation potential are transferred into humans .Higher concentrations of metallic compounds are toxic to living organisms but these are essential to maintain body metabolism. Intake of food crops polluted with heavy metals is chief food chain channel for human exposure. Animals are exposed to heavy metal stress by the intake of richly contaminated food crops those are chief part of food chain. We collected samples of soil, plant, animal blood, hair and faeces to find contamination through wet digestion process in lab and metal analysis. Different forages were collected to study Zn amount in forages whilst soil and animals in Mianwali Pakistan. The health risk index (HRI) calculation was our major concern in this study.Our present findings also emphasized on the assessment of bio-concentration factor (BCF). We also calculated other significant indices i.e. Pollution load index (PLI), daily intake of metal (DIM), Health Risk Index (HRI) and Enrichment factor (EF). While the experimentation result showed different concentrations of metal in different seasons. When the Zn concentration in forages was (32.59-42.17mg/kg) and in soil (21.82-35.09 mg/kg). Soil samples showed higher level of (PLI) Pollution load index. Bio-concentration of zinc was (1.03-1.57mg/kg). It can be concluded as regular monitoring of the level metal is essential evaluate the contamination status.

The aim of study was to access the Mn contamination in soil, forages and animals. Heavy metal pollution is a matter of prime signi cance in natural environment. Through food chain toxicity of heavy metals and their bioaccumulation potential are transferred into humans .Higher concentrations of metallic compounds are toxic to living organisms but these are essential to maintain body metabolism.Intake of food crops polluted with heavy metals is chief food chain channel for human exposure. Animals are exposed to heavy metal stress by the intake of richly contaminated food crops those are chief part of food chain. We collected samples of soil, plant, animal blood, hair and faeces to nd contamination through wet digestion process in lab and metal analysis. Different forages were collected to study Mn calculation was our major concern in this study. Our present ndings also emphasized on the assessment of bio-concentration factor (BCF). We also calculated other signi cant indices i.e. Pollution load index (PLI), daily intake of metal (DIM), Health Risk Index (HRI) and Enrichment factor (EF). While the experimentation result showed different concentrations of metal in different seasons. When the Mn concentration in forages was (20.01-28.29 mg/kg) and in soil (5.27-8.90 mg/kg). Soil samples showed higher level of (PLI) Pollution load index. Bio-concentration of iron was (2.59-4.21 mg/kg). It can be concluded as regular monitoring of the level metal is essential evaluate the contamination status.

The aim of study was access the cobalt and lead contamination in soil, forages and animals. Heavy metal pollution is a matter of prime significance in natural environment. Through food chain toxicity of heavy metals and their bioaccumulation potential are transferred into humans .Higher concentrations of metallic compounds are toxic to living organisms but these are essential to maintain body metabolism. Intake of food crops polluted with heavy metals is chief food chain channel for human exposure. Animals are exposed to heavy metal stress by the intake of richly contaminated food crops those are chief part of food chain. We collected samples of soil, plant, animal blood, hair and faeces to find contamination through wet digestion process in lab and metal analysis. Different forages were collected to study Zn amount in forages whilst soil and animals in Mianwali Pakistan. The health risk index (HRI) calculation was our major concern in this study. Our present findings also emphasized on the assessment of bio-concentration factor (BCF). We also calculated other significant indices i.e. Pollution load index (PLI), daily intake of metal (DIM), Health Risk Index (HRI) and Enrichment factor (EF). While the experimentation result showed different concentrations of metal in different seasons. When the Zn concentration in forages was (32.59-42.17mg/kg) and in soil (21.82-35.09 mg/kg). Soil samples showed higher level of (PLI) Pollution load index. Bio-concentration of zinc was (1.03-1.57mg/kg). It can be concluded as regular monitoring of the level metal is essential evaluate the contamination status.

Background: The aim of study was accessing the cobalt (Co) and lead (Pb) contamination in soil, forages and animals. The heavy metal ions bioaccumulation and escalating transfer to food chain has become main concern for mankind. Nevertheless, they play crucial role in organismal metabolism, however exceeding threshold level causes toxicity or even system failure. Since the plants are the main source of nutrition supply and carbohydrates for animal metabolism, therefore mankind exposure risk to food chain heavy metal toxicity increases significantly. Method: To highlight toxicity levels and exposure risk we designed this field study in the nearby areas of district Mianwali, Punjab, Pakistan during different seasons. and carried out samplings of soil, native flora. While following research ethics we collected samples of blood, hair and feces from domestic herbivore animals. Traditional wet digestion was carried out in available laboratory facilities, Pb and Co heavy metal concentrations were analyzed with atomic absorption spectrum. With emphasis on health risk index (HRI) and bio-concentration factor (BCF), we also draw attention to pollution load index (PLI), daily intake of metal (DIM), Health Risk Index (HRI) and Enrichment factor (EF). Results: Our findings revealed high Pb concentration (5.29-9.84 mg/kg) in forage during season 1. While our findings indicate concentrations of metal in different seasons. When the Co concentration in forages was 0.238 to 0.492 mg/kg and Pb was found in the range of 5.29-9.84 mg/kg). Soil samples showed higher level of (PLI) Pollution load index. Bio-concentration of cobalt and lead was (0.447- 1.106 and 0.717- 1.152 mg/kg) respectively. Conclusions: Bio-concentration factor and pollution load index for Co and Pb was noticed significantly higher than 1. Whereas enrichment factor (EF) was under permissible limit for both metals, daily intake metal (DIM) and no harmful health risk concentration was found. Therefore, wide-scale regular monitoring for heavy metal risk assessment on seasonal basis are highly recommended to avoid or minimize further contamination.

The aim of study was to access the Mn contamination in soil, forages and animals. Heavy metal pollution is a matter of prime significance in natural environment. Through food chain toxicity of heavy metals and their bioaccumulation potential are transferred into humans .Higher concentrations of metallic compounds are toxic to living organisms but these are essential to maintain body metabolism. Intake of food crops polluted with heavy metals is chief food chain channel for human exposure. Animals are exposed to heavy metal stress by the intake of richly contaminated food crops those are chief part of food chain. We collected samples of soil, plant, animal blood, hair and faeces to find contamination through wet digestion process in lab and metal analysis. Different forages were collected to study Mn calculation was our major concern in this study. Our present findings also emphasized on the assessment of bio-concentration factor (BCF). We also calculated other significant indices i.e. Pollution load index (PLI), daily intake of metal (DIM), Health Risk Index (HRI) and Enrichment factor (EF). While the experimentation result showed different concentrations of metal in different seasons. When the Mn concentration in forages was (20.01-28.29 mg/kg) and in soil (5.27-8.90 mg/kg). Soil samples showed higher level of (PLI) Pollution load index. Bio-concentration of iron was (2.59-4.21 mg/kg). It can be concluded as regular monitoring of the level metal is essential evaluate the contamination status.