The aim of the investigation was to determine the potential effect of lead on maize growth. Lead is considered as important potent environmental contaminant. Various ecological, environmental and evolutionary processes in the microsphere are disrupted because of lead toxicity to the microbial community. The effects of Lead Nitrate (Pb(No 3 ) 2 ) as heavy metal on germination, early growth seedling, root-shoot length, root-shoot fresh and dry weights, total protein content and the uptake of lead by roots and shoots of Zea-mays were investigated. All of the parameters were reduced by the increased lead concentrations. Such growth retardation was due to metals toxicity that resulted in damages to various physiological and biochemical processes.
We report that endogenous, as well as exogenous, interferon (IFN) regulates the growth of human melanoma cells in culture. When antibodies directed against human fibroblast IFN were incorporated into the media of high-density cells stimulated to proliferate with serum, the cells entered the cell cycle earlier than did the controls. In investigating the biochemical basis for this finding, we have found that there is an inverse relationship between the (2'-5')oligoadenylate synthetase levels and the percentage of cells in S in untreated cultures. Upon IFN treatment, the relationship is obliterated and (2'-5')oligoadenylate synthetase levels increase throughout all phases of the cell cycle. This increase in enzyme levels correlates well with the decreased probability of the IFN-treated cells to cycle. These findings suggest a biological role for IFN as a negative growth factor for cells in culture.
In the recent years, the use of sewage water for irrigation has attracted the attention of arid and semi-arid countries where the availability of fresh water is poor. Despite the potential use of sewage water in crop irrigation as effective and sustainable strategy, the environmental and human risks behind this use need to be deeply investigated. In this regard, an experiment was carried out under field conditions in Nursery, University College of Agriculture Sargodha, to evaluate the possible health risks of undesirable metals in wheat grains. Wheat variety Sarang was cultivated and irrigated with different combinations of ground (GW) and sewage water (SW). The concentrations of heavy metals (Cr, Cd, Ni, and Pb) and trace elements (Cu, Zn, and Fe) in wheat grains as well as in soil were determined. Moreover, the pollution load index (PLI), accumulation factor (AF), daily intake of metals (DIM), and health risk index (HRI) were calculated. Results showed that the concentration trend of heavy metals was Pb
T HE MAIN objective of the study was to determine the cadmium (Cd) concentrations in vegetables grown in soil irrigated with canal water and sewage water. The samples were analysed by using atomic absorption spectrophotometer (AAS). The mean Cd concentrations in soil ranged between 1.153-2.294mg/kg and the mean Cd concentrations (mg/kg) in vegetables ranged from 0.789 to 1.575, 0.210 to 0.423, 0.264 to 0.523, 0.203 to 0.404, 0.169 to 0.334, 0.223 to 0.443, 0.723 to 1.443 and 0.344 to 1.450mg/kg for Raphanus sativus, Brassica rapa, Zingiber officinale, Capsicum baccatum, Capsicum frutescens, Capsicum annuum, Solanum lycopersicum and Curcuma longa, respectively. The recorded health risk index value for Cd concentration was greater than 1 in each vegetable and these values were higher than the determined permissible limit. In all vegetables, health risk index values for Cd were higher during sewage water treatments as compared to the canal water treatments. Finally, the study showed that bioaccumulation of Cd in vegetable samples was high in the study area.
Investigation was performed to assess the effect of different sewage water treatments on the metal status of different fodder species consumed by ruminants under semi-arid conditions. Five samples each of six fodder species viz., Trifolium alexandrinum, Cichorium intybus, Avena sativa, Medicago polymorpha, Brassica campestris and Medicago sativa were collected from three fields irrigated with canal water, mix water (canal water and sewage water) and sewage water, respectively. Fodder samples were analyzed to determine the Mg, Co and Zn concentrations in shoots. Higher values of these metals were found in fodder species irrigated with sewage water relatively. Mg and Zn concentrations in all the fodder species were found to be below the critical level among all treatments. Whereas, concentrations of Co in the different fodder species were significantly different (p < 0.05) and above the critical level. Consequently, ruminants feeding on these fodder species need continued mineral supplementation of Zn and Mg elements to prevent diseases caused by the deficiency of these elements, and to support optimum animal productivity.
2 The key component for global sustainability is plant diversity, an essential for living sources on this planet. An investigation was carried out in the Soone Valley, Punjab, Pakistan to determine the nutritional value of plant diversity in relation to requirements of grazing ruminants therein. Various parameters such as moisture content, crude protein, mineral matter, fat fiber content, dry matter, nitrogen free extractable substances, and net energy were evaluated in this investigation. Results showed that various plant species studied exhibited fluctuation in all parameters in this valley. Correlation studies also indicated that various parameters showed negative as well as positive significant relationships with various parameters of soil in this valley. The information will be useful in further conservation program. A. farnesiana was negatively correlated (−0.335) with protein and mineral matter (−0.048). Significant correlations of different plant species regarding nitrogen-phosphorus-potassium (NPK) were recorded with net energy. Various variables studied in this investigation demonstrated their importance to be used in further conservation, research, and grazing livestock programs.
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