A method of determining corticosterone concentration in turkey plasma was developed using radioimmunoassay. Compared to fluorometric analysis and competitive protein-binding radioassay, this method had the following advantages: 1) results were consistent, 2) the method was simple and rapid to perform, 3) only 20 lambda of plasma was required, 4) all procedures were performed using the same tube except for counting, and 5) unknown and standard samples were treated identically. The coefficient of variation with the 5 ng/ml standard sample between 14 sets of assays was 12.6% and the average coefficient of variation within duplicate assays of unknown samples randomly selected from each of the 14 series was 4%. Using this method, there was an increase in the average plasma corticosterone concentration in all groups of inoculated turkeys one day after inoculation of Pasteurella multocida which was significantly (P less than .05) greater than that of the noninoculated groups.
Inflammation is frequent at the site of injection in turkeys vaccinated with various commercial fowl cholera bacterins. Seven commercial and three laboratory bacterins were compared in 12week-old turkeys as to extent and severity of irritation with 3 routes of injection: subcutaneous, intradermal, and intramuscular. Commercial fowl cholera bacterins, particularly those with paraffin oil as the adjuvant, varied greatly in the irritation produced. The amount appeared consistent with the bacterin or adjuvant and somewhat independent of route of injection. The reaction to selected bacterins was comparable in 4-week-old turkeys to that in 12-weekolds. The reaction was usually less severe at 15 days postinjection than at 5 days. The intradermal route gave the most consistent results, probably because less bacterin diffused from the injection site, yet the amount of reaction was still not sufficiently consistent to be used quantitatively. Reaction to a given bacterin or adjuvant varied the most with the subcutaneous route.
The chromosomal DNA of 29 field isolants of Pasteurella multocida from commercial turkey farms in Missouri and the avirulent Clemson University (CU) and M-9 vaccine strains of P. multocida were tested using the arbitrarily primed polymerase chain reaction (AP-PCR) in combination with 32P-labeled deoxycytidine triphosphate (dCTP) and high-resolution gel electrophoresis. The 29 field isolants of P. multocida were isolated from outbreaks of fowl cholera in turkey flocks in which vaccination with the CU vaccine had been performed within 2 weeks of the isolation, and it was suspected that the outbreak could have been due to the use of the live CU vaccine. The results of this study showed that: 1) the use of the live CU vaccine can lead to the isolation of the vaccine strain if the outbreak occurs within 2 weeks of vaccination; 2) a higher proportion of field isolants collected during 1983 and 1984, when the usage of the CU vaccine strain was highest on Missouri turkey farms, had PCR-amplified product profiles similar or identical to those of the CU vaccine strain compared with the period between 1987 and 1992, when its use was less; and 3) there was no relationship between the PCR-amplified product profiles and the serotype.
A comparative study was made of stained fecal smears and cultured fecal swabs for identification of the large spirochetes Treponema hyodysenteriae and Treponema innocens. Feces were obtained by swabbing rectums, colons, and stools of nonexposed swine and swine experimentally exposed to swine dysentery. In this study there was a significant (P < 0.001) correlation between the observation of one or more large spirochetes on stained slides and obtaining either a strong or a weak beta-hemolytic reaction in culture. A significant (P < 0.001) correlation was also found between the observation of one or more large spirochetes on stained smears or obtaining either a strong or a weak beta-hemolytic reaction in culture and the occurrence of either nonhemorrhagic or hemorrhagic diarrhea in the swine. In the diarrheic swine at the time of swabbing, 157 of 393 samples (40%) were negative for both the presence of large spirochetes on stained smears and the production of either a strong or a weak beta-hemolytic reaction; in nondiarrheic swine, 42 of 278 samples (15.1%) were positive in stained smears and 32 of 268 samples (11.9%) were positive by culturing. In swine infected with swine dysentery, 17 of 1,011 samples produced weak beta-hemolytic reactions, and in swine infected with nonpathogenic large spirochetes of T. innocens, three of 34 samples produced strong beta-hemolytic reactions. It was concluded from this study that neither staining rectal smears nor culturing rectal swabs is sufficient, either together or alone, for the diagnosis of swine dysentery; however, these laboratory methods could be highly supportive of a diagnosis of swine dysentery in swine with clinical signs and lesions of the disease.
Ketamine hydrochloride was observed to be an effective anesthetic for recently captured raccoons (Procyon lotor) when they were injected intramuscularly with 20-29 mg/kg body weight. Excellent anesthesia occurred from 5 to 15 min after injection. No respiratory difficulties were encountered. The only undesirable clinical sign was excessive salivation.
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