The infectivity of three viruses in leaf material dried over CaC12 and stored under different conditions was tested over a period of seven years. Alfalfa mosaic virus and cucumber mosaic virus kept very well for the entire period both at 4~ and at room temperature, provided they were stored over CaCI2 in well-closed tubes. The infectivity of bean yellow mosaic virus, however, gradually declined, especially at room temperature. In open or loosely closed containers without CaCI2 none of the viruses survived more than three months of storage and the infectivity of bean yellow mosaic virus declined after only two weeks.
A virus isolate obtained from red clover in the Netherlands in 1957 has been identified as red clover mottle virus by means of host reaction studies, electron microscopy and serology. This isolate is considered to be a separate strain, however, on the basis of clear differences in severity of symptoms in broad bean, pea and red clover plants and especially of qualitative serological differences between this isolate and the English type strain. The virus produces granular inclusion bodies in artificially infected pea plants. It is especially distinguished from other legume viruses described so far by its reaction in broad bean, pea, and red clover plants. A discussion is given of its distant relationship to cowpea mosaic virus. This is the first report of the occurrence of the red clover mottle virus outside England. At present the virus appears to be of no economic importance in the Netherlands.
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