Persistence of infectivity of three viruses in plant material dried over CaCl2 and stored under different conditions

Bos, L.

doi:10.1007/bf01977034

Cited by 17 publications

(8 citation statements)

References 1 publication

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“…Out of 33 successfully co-infected plants, ten randomly selected plants were grown for a total of four months before leaf samples were collected and stored as previously described by Bos [36].…”

Section: Methodsmentioning

confidence: 99%

Complex Recombination Patterns Arising during Geminivirus Coinfections Preserve and Demarcate Biologically Important Intra-Genome Interaction Networks

et al. 2011

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Genetic recombination is an important process during the evolution of many virus species and occurs particularly frequently amongst begomoviruses in the single stranded DNA virus family, Geminiviridae. As in many other recombining viruses it is apparent that non-random recombination breakpoint distributions observable within begomovirus genomes sampled from nature are the product of variations both in basal recombination rates across genomes and in the over-all viability of different recombinant genomes. Whereas factors influencing basal recombination rates might include local degrees of sequence similarity between recombining genomes, nucleic acid secondary structures and genomic sensitivity to nuclease attack or breakage, the viability of recombinant genomes could be influenced by the degree to which their co-evolved protein-protein and protein-nucleotide and nucleotide-nucleotide interactions are disreputable by recombination. Here we investigate patterns of recombination that occur over 120 day long experimental infections of tomato plants with the begomoviruses Tomato yellow leaf curl virus and Tomato leaf curl Comoros virus. We show that patterns of sequence exchange between these viruses can be extraordinarily complex and present clear evidence that factors such as local degrees of sequence similarity but not genomic secondary structure strongly influence where recombination breakpoints occur. It is also apparent from our experiment that over-all patterns of recombination are strongly influenced by selection against individual recombinants displaying disrupted intra-genomic interactions such as those required for proper protein and nucleic acid folding. Crucially, we find that selection favoring the preservation of co-evolved longer-range protein-protein and protein DNA interactions is so strong that its imprint can even be used to identify the exact sequence tracts involved in these interactions.

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Section: Methodsmentioning

confidence: 99%

Complex Recombination Patterns Arising during Geminivirus Coinfections Preserve and Demarcate Biologically Important Intra-Genome Interaction Networks

et al. 2011

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“…Sampling and DNA extraction. Tomato (Solanum lycopersicon), tobacco (Nicotiana tabaci) and bean (Phaseolus vulgaris) leaves presenting leaf curling symptoms were collected from individual plants on the islands of the Comoros archipelago (Anjouan, Grande Comore, Mayotte and Moheli), the Seychelles archipelago (Mahé) and Madagascar (Table 1) and stored dried (Bos, 1977). Total DNA was extracted using a DNeasy Plant miniprep kit (Qiagen) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Begomovirus ‘melting pot’ in the south-west Indian Ocean islands: molecular diversity and evolution through recombination

Lefeuvre

Martin

Hoareau

et al. 2007

Journal of General Virology

166

128

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During the last few decades, many virus species have emerged, often forming dynamic complexes within which viruses share common hosts and rampantly exchange genetic material through recombination. Begomovirus species complexes are common and represent serious agricultural threats. Characterization of species complex diversity has substantially contributed to our understanding of both begomovirus evolution, and the ecological and epidemiological processes involved in the emergence of new viral pathogens. To date, the only extensively studied emergent African begomovirus species complex is that responsible for cassava mosaic disease. Here we present a study of another emerging begomovirus species complex which is associated with serious disease outbreaks in bean, tobacco and tomato on the south-west Indian Ocean (SWIO) islands off the coast of Africa. On the basis of 14 new complete DNA-A sequences, we describe seven new island monopartite begomovirus species, suggesting the presence of an extraordinary diversity of begomovirus in the SWIO islands. Phylogenetic analyses of these sequences reveal a close relationship between monopartite and bipartite African begomoviruses, supporting the hypothesis that either bipartite African begomoviruses have captured B components from other bipartite viruses, or there have been multiple B-component losses amongst SWIO virus progenitors. Moreover, we present evidence that detectable recombination events amongst African, Mediterranean and SWIO begomoviruses, while substantially contributing to their diversity, have not occurred randomly throughout their genomes. We provide the first statistical support for three recombination hot-spots (V1/C3 interface, C1 centre and the entire IR) and two recombination cold-spots (the V2 and the third quarter of V1) in the genomes of begomoviruses.

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“…Tomato leaves presenting leafcurling symptoms were collected from individual plants in Saint Pierre (Réunion), Morondova and Toliary (Madagascar), and Dembeni and Kahani (Mayotte). The leaves were preserved by dehydration with CaCl 2 (Bos, 1977). Total DNA was extracted from dried samples using the DNeasy Plant miniprep kit (Qiagen) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

South West Indian Ocean islands tomato begomovirus populations represent a new major monopartite begomovirus group

Delatte

Martin

Naze

et al. 2005

Journal of General Virology

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, were shown to be both infectious in tomato and transmissible by Bemisia tabaci. Sequence analysis revealed that these viruses had genome organizations of monopartite begomoviruses and that both ToLCMGV and ToLCYTV belong to the African begomoviruses but represent a distinct monophyletic group that we have tentatively named the South West islands of the Indian Ocean (SWIO). All of the SWIO isolates examined were apparently complex recombinants. None of the sequences within the recombinant regions closely resembled that of any known non-SWIO begomovirus, suggesting an isolation of these virus populations.

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Persistence of infectivity of three viruses in plant material dried over CaCl2 and stored under different conditions

Cited by 17 publications

References 1 publication

Complex Recombination Patterns Arising during Geminivirus Coinfections Preserve and Demarcate Biologically Important Intra-Genome Interaction Networks

Complex Recombination Patterns Arising during Geminivirus Coinfections Preserve and Demarcate Biologically Important Intra-Genome Interaction Networks

Begomovirus ‘melting pot’ in the south-west Indian Ocean islands: molecular diversity and evolution through recombination

South West Indian Ocean islands tomato begomovirus populations represent a new major monopartite begomovirus group

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