In general, it can be stated that gellan macro beads may be regarded as suitable for site specific delivery of NPX to the colon. However, these simple to obtain beads can be potentially used as carriers for many different drugs whenever it is necessary to omit the stomach.
Bacteriophage potential in combating bacterial pathogens has been recognized nearly since the moment of discovery of these viruses at the beginning of the 20th century. Interest in phage application, which initially focused on medical treatments, rapidly spread throughout different biotechnological and industrial fields. This includes the food safety sector in which the presence of pathogens poses an explicit threat to consumers. This is also the field in which commercialization of phage-based products shows the greatest progress. Application of bacteriophages has gained special attention particularly in recent years, presumably due to the potential of conventional antibacterial strategies being exhausted. In this review, we present recent findings regarding phage application in fighting major foodborne pathogens, including Salmonella spp., Escherichia coli, Yersinia spp., Campylobacter jejuni and Listeria monocytogenes. We also discuss advantages of bacteriophage use and challenges facing phage-based antibacterial strategies, particularly in the context of their widespread application in food safety.
In this paper, several
experimental techniques [X-ray diffraction,
differential scanning calorimetry (DSC), thermogravimetry, Fourier
transform infrared spectroscopy, and broad-band dielectric spectroscopy]
have been applied to characterize the structural and thermal properties,
H-bonding pattern, and molecular dynamics of amorphous bosentan (BOS)
obtained by vitrification and cryomilling of the monohydrate crystalline
form of this drug. Samples prepared by these two methods were found
to be similar with regard to their internal structure, H-bonding scheme,
and structural (α) dynamics in the supercooled liquid state.
However, based on the analysis of α-relaxation times (dielectric
measurements) predicted for temperatures below the glass-transition
temperature (
T
g
), as well as DSC thermograms,
it was concluded that the cryoground sample is more aged (and probably
more physically stable) compared to the vitrified one. Interestingly,
such differences in physical properties turned out to be reflected
in the lower intrinsic dissolution rate of BOS obtained by cryomilling
(in the first 15 min of dissolution test) in comparison to the vitrified
drug. Furthermore, we showed that cryogrinding of the crystalline
BOS monohydrate leads to the formation of a nearly anhydrous amorphous
sample. This finding, different from that reported by Megarry et al.
[
21492830
Carbohydr. Res.
2011
346
1061
1064
] for trehalose (TRE), was revealed on the
basis of infrared and thermal measurements. Finally, two various hypotheses
explaining water removal upon cryomilling have been discussed in the
manuscript.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.