Breeding and rearing some of the clownfishes most commonly used in the aquarium trade actually represent an economical and ecological tool for broadening development. Culture of clownfish species in low-saline water is still in its infancy. Salinity of the culture environment is one of the more relevant parameters affecting fish physiology, modifying food intake and growth performance in many fish species. The objective of this study was to breed skunk clownfish (Amphiprion akallopisos) in aquarium condition, document the embryonic development, determine the upper and lower lethal salinities of juveniles, tolerance of five different salinities (20, 25, 30, 35, and 40 ppt) and their effect on the survival rate of larvae. Higher (53-55 ppt) and lower (3-6 ppt) salinities produced loss of appetite and movement, finally leading to mortality in juveniles. In a ninety six hour experiment, larvae showed 100% survival at the salinities of 30 (control) and 35 ppt and 88% survival in 40 ppt salinity and 76% survivals in 20 and 25 ppt. In conclusion juveniles of A. akallopisos exhibit satisfactory rates of survival and no signs of stress in high (up to 53 ppt) and low saline (up to 6 ppt) waters. These results demonstrate that using such salinities, which can reduce the incidence of diseases and mortality, does not produce significant physiological alterations in this species. In addition, descriptive studies on embryonic development and mass scale larval rearing were also carried out during the present study.
The aim of the present study is conducted to evaluate the antioxidant potential and toxicity effect of polysaccharide-fucoidan from the brown seaweed Sargassum tenerrimum. Fucoidan-a sulphated polysaccharide contains fucose and sulphate as major compounds. Interestingly, various studies reported that the presence of sulphate content of the sample plays a significant role in pharmacological activities. In this study, fucoidan was fractionated by Ion exchange chromatography method and it major chemical constituent sulphate and fucose was determined by the biochemical methods. The toxicity effect of fucoidan was analyzed by the brine shrimp toxicity assay. Three fucoidan fractions (F1, F2 and F3) were obtained from Intact Fucoidan (IF) through anion-exchange column chromatography. In vitro antioxidant capability was analyzed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radical scavenging and total antioxidant assays and intact fucoidan showed the maximum activity 83.66 +/- 0.35, 81.73 +/- 0.35% and 41.6 +/- 0.43 mg g(-1), respectively. The finding of the present study was confirmed that the antioxidant property of fucoidan was depending upon the sulphate content of the fraction and these studies proved that fucoidan have non toxicity effect. Hence, fucoidan have the scope of being used as natural antioxidants in treating many human diseases.
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