In vitro and in vivo efficacy of partially purified herbal extracts against bacterial fish pathogens

Devi, K. Nanthini; Dhayanithi, Nagarajan Balachandran; Kumar, T. T. Ajith; Balasundaram, Chellam; Harikrishnan, Ramasamy

doi:10.1016/j.aquaculture.2016.02.035

Cited by 19 publications

(8 citation statements)

References 45 publications

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“…Similarly, feeding Caspian brown trout with peppermint ( Mentha piperita L.) extract enhanced the mucosal and humoral immune parameters and growth performance indexes (Adel, Abedian Amiri, et al, 2015). Devi, Dhayanithi, Kumar, Balasundaram, & Harikrishnan, (2016) found that administration of the extracts of seagrass ( Cymodocea serrulata ), turmeric ( Curcuma longa ), Spirulina ( Spirulina platensis ) and beetroot ( Beta vulgaris ) could promote antimicrobial and antioxidant activities in the blood of Pseudochromis dilectus against Proteus mirabilis infection. Also, yellowtail kingfish, Seriola lalandi , fed on garlic powder, rosemary extract and rosemary oil in pellets showed reduced Zeuxaptaseriolae abundance on the gills after 20 days of feeding (Ingelbrecht et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

The potential benefits of Mentha longifolia on growth performance and innate immunity parameters in Caspian kutum ( Rutilus frisii kutum )

et al. 2020

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The current research was conducted to examine the benefits of Mentha longifolia (ML) administration through diet on growth performance and haematological, biochemical, and immunological parameters of Caspian kutum (Rutilus frisii kutum). Fish with average weight of 3 g were fed for 8 weeks with diets supplemented with 0%, 2%, 4% and 6% ML‐extract. Feeding with 6% ML for 8 weeks significantly improved the growth performance. The highest values in total protein content, protease and esterase activities were observed in the 6% ML. Feeding with 4% and 6% ML led to a significant increase in the alternative complement, respiratory burst activities and total immunoglobulins in blood as well as lysozyme activity in the mucus and serum. The enhancement of skin mucus immune parameters was observed in fish fed with dietary (≥4%) ML supplement. The mucosal antibacterial effects revealed that the highest ML concentration (6%) had the maximum bacterial growth inhibition zone diameter (mm) and MIC compared with other groups in the study. White blood cell numbers and haematocrit values were significantly higher than in the control groups following the use of 4% and 6% ML. The highest red blood cell counts resulted from the use of 6% ML. Although 4% and 6% ML led to a decrease in the number of lymphocytes, the neutrophils increased after using the same dose, compared with the control group. Triglycerides levels significantly decreased in fish fed with 4% and 6% ML; however, no significant changes were observed in other blood biochemical parameters. Thus, it can be concluded that diets supplemented with ML‐extract could significantly impact Caspian kutum by improving growth performance and boosting their immune response.

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Section: Introductionmentioning

confidence: 99%

The potential benefits of Mentha longifolia on growth performance and innate immunity parameters in Caspian kutum ( Rutilus frisii kutum )

et al. 2020

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“…Recently, the popularity of plant extracts as natural immunostimulant is gaining in demand and importance in medical purposes. Various plant extracts, such as Indian almond leaves ( Terminalia catappa ), oats ( Avena sativa ), oyster mushroom ( Pleurotus ostreatus ), nettle ( Urtica dioica ), sea grass ( Cymodocea serrulata ) and beetroot ( Beta vulgaris ) have been used as alternatives to antibiotics 5, 14– 16 . Plant extracts also contain levamisole 13 and saponin 17 which can enhance the work of nonspecific immune systems and increase the activation of phagocytosis 14 .…”

Section: Introductionmentioning

confidence: 99%

Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus)

et al. 2019

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Background: The combination of some plant extracts to prevent and treat bacterial infections is gaining momentum, because of effectiveness against certain bacteria. This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zingiber Zerumbet (ZZ) plant extracts to treat and prevent Aeromonas hydrophila and Pseudomonas fluorescens infection on Tilapia (Oreochromis niloticus). Methods: Tilapia (initial weight 15±2 g) were injected intramuscularly (0.1 ml/fish) with a combination of A. hydrophila and P. fluorescens at a density of 1×10 5 CFU ml -1 of each bacteria. Treatment trials were performed at day 7 post-injection with each combined extract, while the prevention trial was performed by including the combined extract into the commercial diet for six and seven days prior to injection. Various extract combinations were 60 mg SF extract/kg feed with 40 mg ZZ/kg feed (SF60/ZZ40), SF50/ZZ50, BP90/SF10, and BP50/SF50. Haemato-immunological parameters were performed for four weeks. Results: In prevention trials, tilapia fed SF50/ZZ50 showed a significant increase of white and red blood cells. Similarly, significantly increased haematocrit was found in tilapia fed SF50/ZZ50 in the treatment trial but not in the prevention trial. In both trials, haemoglobin of tilapia was not affected by any combined extracts but decreased the number of bacteria. Phagocytic index, respiratory burst, lysozyme activity and survival rate of fish fed combined extracts were found significantly higher than controls. The amount of pathogenic bacteria in fish fed combined extracts was lower than the control at week 4 (P<0.05). In both trials The percentage of survival rate and relative percent survival of tilapia fed SF 50/ZZ 50, showed the optimum results compared to the other combinations. Conclusions: The combined extract in feed, especially SF50/ZZ50 has a positive effect on the tilapia's innate immune system of tilapia to treat and prevent bacterial infections.

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“…Symptoms caused by the two bacteria are skin ulcers in fish. In addition, these bacteria are also found in fish teguments, intestines, and fresh fillets [17]. Spingomonas paucimobilis is an organism that is often found in the environment, namely from water, soil, and other sources.…”

Section: Discussionmentioning

confidence: 99%

The Abundance of Pathogenic Bacteria in Response to The Administration of Fermented Herbal Extract Vitomolt Plus into The Tilapia Cultivation Water

Sari

Fujaya

Sriwulan

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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The purpose of this study was to analyze the effect of various concentrations of vitomolt plus added to the culture water on the abundance of pathogen bacteria. This research was conducted in August - September 2021 in Makassar, Indonesia. There were 18 containers with 30 L of volume used in this study. Juvenile tilapia about 5 cm of body length were stocked with a density of 5 fish for each container. The experimental design were designed in a completely randomized design with 6 concentrations of vitomolt treatment, namely: A (control), B (2 ppm), C (4 ppm), D (6 ppm), E (8 ppm), F (10 ppm). Each treatment consisted of 3 replications. The parameters observed were the total abundance of bacteria, types of bacteria, and the percentage of identified bacterial abundance. The results showed that the dose of Vitomolt plus treatment had an effect (P<0.05) on the total abundance of bacteria, where the concentration of 6 ppm vitomolt plus had the lowest average total bacterial abundance and the control was the highest. There were several bacteria identified with an average abundance starting from the highest, respectively, including Yersinia pseudotuborculosis, Aeromonas sobria, Staphylococcus sciuri, Spingomonas paucimobilis, Staphylococcus haemolyticus, Micrococcus luteus, and Aeromonas hydrophila. Based on the results, it can be concluded that the administration of 6 ppm vitomolt plus into the water of tilapia aquaculture has an effect on the abundance of pathogenic bacteria. The use of fermented herbal extracts can be considered as anti-bacterial in aquaculture.

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In vitro and in vivo efficacy of partially purified herbal extracts against bacterial fish pathogens

Cited by 19 publications

References 45 publications

The potential benefits of Mentha longifolia on growth performance and innate immunity parameters in Caspian kutum ( Rutilus frisii kutum )

The potential benefits of Mentha longifolia on growth performance and innate immunity parameters in Caspian kutum ( Rutilus frisii kutum )

Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus)

The Abundance of Pathogenic Bacteria in Response to The Administration of Fermented Herbal Extract Vitomolt Plus into The Tilapia Cultivation Water

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