Background. This study describes antimicrobial, anticancer and antioxidant properties of Sauropus androgynus stem extract. The main objective of the present study was to reveal the potential of S. androgynus strip to be used as a medicinal drug.Materials and methods. The antimicrobial properties of S. androgynus stem extract against Aeromonas hydrophila, Escherichia coli, Edwardsiella tarda, Flavobacterium sp., Klebsiella sp., Salmonella sp., Vibrio alginolyticus, V. parahaemolyticus, V. cholerae and Pseudomonas aeruginosa were revealed by using the broth micro-dilution method, whereas the anticancer effects of the extract was determined with a colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF-7). The antioxidant activity of the plant extract was characterized also by using a α, α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging method. Finally, chemical compounds of the plant extract were screened and identified by using gas chromatography -mass spectrometry (GC-MS).Results. The minimum inhibitory concentration (MIC) values ranged from 7.81 to 62.5 mg/l in which the plant extract was found to inhibit the growth of Edwardsiella tar da, Escherichia coli, Flavobacterium sp., Pseudomonas aeruginosa and Vibrio cholerae at 7.81 mg/l, Klebsiella sp., Aeromonas hydrophila and Vibrio alginolyticus at 15.6 mg/l, and it was able to control the growth of Salmonella sp. and Vibrio parahaemolyticus at 62.5 mg/l. The results of the present study has shown S. androgynus stem extract to possess a high antimicrobial activity and a moderate antioxidant activity (inhibition concentration 50%; IC 50 of DPPH is 8 ppt), but no anticancer activity. A total of 34 compounds were identified in the plant extract in which the major compounds were 9, 12, 15-octadecatrienoic acid, methyl ester, (Z, Z, Z)-(14.48%) and phytol (13.08%).Conclusions. S. androgynus stem extract can be used as an antimicrobial and antioxidant agents.
Background: Microalgae is one of the major sources of natural compounds with antimicrobial activity. The metabolite profiling of the extracts could identify the bioactive compounds based on methanol (MET), ethanol (ETH), chloroform (CHL), hexane (HEX) and water (W) solvent systems. The microalgal crude extracts in co-application with silver nanoparticles (AgNPs) had enhanced antimicrobial activity with potential to overcome the global problem of microbial antibiotic resistance. Results: Chlorella sp. exhibited the highest lipid, N. oculata the highest total saturated fatty acids (TSFA), and T. suecica the highest mono-unsaturated (MUFA) and poly-unsaturated fatty acids (PUFA). The highest carbohydrate, protein and total phenolics contents (TPCs) were attained by N. oculata. The highest total flavonoids contents (TFCs), and chlorophyll a and b were in T. suecica, while comparable level of carotenoids were found in all species. For high-performance thin-layer chromatography (HPTLC) analyses, the eicosapentaenoic acid (EPA) with high peaks were detected in T. suecica-HEX and N. oculata-CHL; and β-carotene in Chlorella sp.-ETH. The gas chromatography-mass spectrometry (GC-MS) analyses showed high 13-docosenamide (Z)-in T. suecica-HEX; phytol in N. oculata-HEX; and neophytadiene in Chlorella sp.-ETH. The AgNPs-MCEs-MET and HEX at the 1.5:1 ratios exhibited strong activities against Bacillus subtilis, Streptococcus uberis, and Salmonella sp.; and the AgNPs-T. suecica-HEX and MET and AgNPs-Chlorella sp.-HEX at the 1.5:1 ratios exhibited activities against Klebsiella pneumoniae. Conclusion: Different bioactive components were detected in the MCEs based on the HPTLC and GC-MS analyses. Significant antimicrobial activities against the pathogenic microbes were demonstrated by the synergistic effects of the MCEs in co-application with the AgNPs. This could be beneficial in the fight against sensitive and multidrugresistant bacteria.
This paper described antimicrobial, antioxidant and anticancer activities of Ficus deltoidea Jack. leaf extract as well as its chemical composition. The main objective of the present study was to reveal the potential of F. deltoidea leaf to be used as medicinal drug. Antimicrobial property of F. deltoidea leaf extract was revealed by using two fold dilution method whereas antioxidant activity of the extract was determined with α, α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging method. The anticancer property of the plant extract was revealed through Colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF-7). Chemical compounds of the plant extract were screening and identified by using gas chromatography-mass spectrometry (GC-MS). The present study result showed that minimum inhibitory concentration (MIC) values of the plant extract against the tested bacterial isolates ranged from 31.26 to 125 mg/ l in which the plant extract can inhibit all the tested bacterial isolates. At the maximum concentration of the plant extract, it was only able to inhibit almost 30 % of DPPH while no anticancer activity was observed against MCF-7 cell. A total of 19 chemical compounds were successfully identified with the phenol 27.12 % and 2,4-bis (dimethylbenzyl)-6-t-butylphenol 11.83 % as the major compounds.
Biofouling is defined as the excessive colonization process of epibiotic organisms, ranging from microfoulers to macrofoulers, on any submerged surface in water. Previous research has attempted to explore the antifouling activity of bacterial isolates due to the biofouling problems occurring worldwide. One solution is to inhibit the early stage of fouling using secondary metabolites produced by marine bacteria. This study aims to determine the antifouling activities of the marine microorganism P. aeruginosa and to characterize the bacteria isolated as a potential anti-biofouling agent. The bacterial isolate was cultured and isolated on a media culture. The bacteria culture extract was extracted using ethyl acetate and concentrated prior to the bioassay method. It was screened for antibacterial activities against Gram-positive and Gram-negative bacteria, such as Bacillus cereus, Streptococcus uberis, Pseudomonas sp., and Vibrio parahaemolyticus, using the disk diffusion technique. The extract was investigated to verify its bioactivity in the prevention of biofilm formation following the crystal violet assay and aquarium test. The results indicated the inhibition of activity through biofilm formation, with the highest percentage at 83% of biofilm inhibition at a concentration of 0.1563 mg/mL. The bacterial isolate at a concentration of 5% showed the highest reduction in bacteria colonies in the aquarium test (161.8 × 103 CFU/mL compared to 722.5 × 103 CFU/mL for the blank sample). The bacterial isolate was characterized through phenotypic and genotypic tests for species identification. It was identified as a Gram-stain-negative, aerobic, and long-rod-shaped bacteria, designated as RLimb. Based on the 16S rDNA gene sequencing analysis, RLimb was identified as Pseudomonas aeruginosa (accession number: OP522351), exhibiting a similarity of 100% to the described neighbor P. aeruginosa strain DSM 50071. These results indicated that these isolated bacteria can potentially be used as a substitute for toxic antifoulants to prevent the formation of microfoulers.
This study was carried out to characterize antimicrobial, antioxidant and anticancer activities of Piper betle leaf extract as well as its chemical composition. The main objective of the present study is to reveal the potential of P. betle leaf to be used as a medicinal drug. Antimicrobial property of P. betle leaf extract revealed by using two fold microdilution method whereas antioxidant activity of the extract was determined with α, α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging method. The anticancer property of the plant extract was revealed through Colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF-7). Chemical compounds of the plant extract were screening and identified by using gas chromatography-mass spectrometry (GC-MS). The result of the present study showed that the minimum inhibition concentration (MIC) values of the plant extract against the tested bacterial isolates ranged from 7.81 to 31.25 mg/l in which the plant extract was found inhibited the growth of all the tested bacterial isolates namely A. hydrophila, E. tarda, E. coli, Flavobacterium sp., Klebsiella sp., P. aeruginosa, Salmonella sp., V. alginolyticus, V. cholerae and V. parahaemolyticus. The value IC50 of the plant extract against DPPH and MCF-7 cells was 4.41 ± 0.03 ppt and 19.4 ± 0.3 μg/ml, respectively. A total of 25 chemical compounds was successfully identified where Benzoic acid 40.44 % and Phytol 14.52 % were the major compounds.The findings of the present study indicated that the plant extract medicinal values are promising.
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