Characterization of antimicrobial, antioxidant, anticancer properties and chemical composition of Sauropus androgynus stem extract

Abstract

Background. This study describes antimicrobial, anticancer and antioxidant properties of Sauropus androgynus stem extract. The main objective of the present study was to reveal the potential of S. androgynus strip to be used as a medicinal drug.Materials and methods. The antimicrobial properties of S. androgynus stem extract against Aeromonas hydrophila, Escherichia coli, Edwardsiella tarda, Flavobacterium sp., Klebsiella sp., Salmonella sp., Vibrio alginolyticus, V. parahaemolyticus, V. cholerae and Pseudomonas aeruginosa were revealed by using the broth micro-dilution method, whereas the anticancer effects of the extract was determined with a colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF-7). The antioxidant activity of the plant extract was characterized also by using a α, α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging method. Finally, chemical compounds of the plant extract were screened and identified by using gas chromatography -mass spectrometry (GC-MS).Results. The minimum inhibitory concentration (MIC) values ranged from 7.81 to 62.5 mg/l in which the plant extract was found to inhibit the growth of Edwardsiella tar da, Escherichia coli, Flavobacterium sp., Pseudomonas aeruginosa and Vibrio cholerae at 7.81 mg/l, Klebsiella sp., Aeromonas hydrophila and Vibrio alginolyticus at 15.6 mg/l, and it was able to control the growth of Salmonella sp. and Vibrio parahaemolyticus at 62.5 mg/l. The results of the present study has shown S. androgynus stem extract to possess a high antimicrobial activity and a moderate antioxidant activity (inhibition concentration 50%; IC 50 of DPPH is 8 ppt), but no anticancer activity. A total of 34 compounds were identified in the plant extract in which the major compounds were 9, 12, 15-octadecatrienoic acid, methyl ester, (Z, Z, Z)-(14.48%) and phytol (13.08%).Conclusions. S. androgynus stem extract can be used as an antimicrobial and antioxidant agents.

A Review on Pharmacognostic, Phytochemical and Ethnopharmacological Findings of Peperomia Pellucida (L.) Kunth: Pepper Elder

“…The compound 7,8-trans-8,8 / -trans-7 / ,8 / -cis-7,7 / -bis(5-methoxy-3,4-methylenedioxyphenyl)-8-acetoxymethyl-8 / -hydroxymethyltetrahydrofuran had exhibited estrogen-like properties in MCF-7 cell line 30,43 . Anticancer activity of P. pellucida leaf extract had also been observed through Colorimetric MTT (tetrazolium) assay against MCF-7 cell line with the half maximal inhibitory concentration (IC50) of 10.4 ± 0.06 μg/mL 38 .…”

“…Phytol had been identified as the major bioactive compound and 2-Naphthalenoldecahydrohexadecanoic acid, methyl ester and 9,12-Octadecadienoic acid (Z, Z)-methyl ester had been identified as the other phytochemicals presented GC/MS 38,49 . Bayma et al; 2000, had isolated a novel dimeric ArC2 compound named Pellucidin A, together with the known phenylpropanoid dillapiole from the methanol extract of aerial parts of P. pellucida.…”

“…pellucida has shown a broad spectrum of antibacterial activity against gram-positive bacteria; Staphylococcus aureus, Bacillus subtilis 13,22,36 and gram-negative bacteria; Escherichia coli 13,22,36,38 , Pseudomonas aeruginosa 13,22,30,36,38 40 . Another study has revealed the potential of the macerated methanol extract of this plant as an antimalarial against Plasmodium falciparum using Desjardin method 41 .…”

“…Antioxidant activity of the crude extracts of this plant had been determined using 2,2-diphenyl-picryhdrazyl (DPPH) radical scavenging assay 30,38,45 . Another study had determined the high antioxidant activity of P. pellucida even at low concentrations using the scavenging effect on DPPH, hydroxyl radical and ferric thiocyanate compared to the antioxidant standards; butylated hydroxyl anisole (BHA), ascorbic acid, and α-tocopherol 36 .…”

Effect of Gamma Irradiation on Angiotensin Converting Enzyme Inhibition, Antioxidant Activity, Total Phenolic Compound and Total Flavonoid of Peperomia pellucida Herbs Extract

“…These are antioxidant, antimicrobial, anticancer, analgesic, anti-inflammatory, and antihypertensive activities. [7][8][9][10][11] The ethanolic extract of the plan has antihypertensive activity through inhibition of the angiotensin-converting enzyme (ACE) in vitro with EC 50 value of 7.17 mg/mL were compared with captopril (13.68 pg / mL). 11 Irradiation can influence the levels of phytochemicals depending on the applied dose, moisture of material, exposure time, and the sensitivity of the phytochemicals towards irradiation and the nature of the raw material used.…”