Effect of Gamma Irradiation on Angiotensin Converting Enzyme Inhibition, Antioxidant Activity, Total Phenolic Compound and Total Flavonoid of Peperomia pellucida Herbs Extract

Adhitia, Anies Monica; Octaviani, Alisa Nur; Rissyelly,; Basah, Katrin; Mun’im, Abdul

doi:10.5530/pj.2017.2.41

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…Based on the results of docking analysis, compound 5 has the most similar interaction with the captopril ligand where the compound binds to the active side of ACE as can be seen in Table 2, and these prediction results are in accordance with the molecular docking results of polyphenol compounds from Phyllanthus niruri herbs (Ahmad et al, 2018). In addition, some study has reported that extracts and fractions (with rich in polyphenol content) of this herbs have activity as an ACE inhibitor using in vitro assay method (Adhitia et al, 2017;Rinayanti et al, 2013;Saputri et al, 2015). Meanwhile, according to the previous study, Kurniawan et al (2016) have done the assay of ACE inhibitory activity of Compound 9 (quercetin) with IC 50 of 16.21 nM which has excellent correlation with the obtained docking result.…”

Section: Resultsmentioning

confidence: 99%

Angiotensin-converting enzyme inhibitory activity of polyphenolic compounds from Peperomia pellucida (L) Kunth: An in silico molecular docking study

Ahmad

Azminah

Mulia³

et al. 2019

J App Pharma Sci

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This study aimed to predict the potential activity and interaction conformation of polyphenolic compounds from Peperomia pellucida (L) Kunth (nine compounds) with angiotensin-converting enzyme (ACE) macromolecule by in silico molecular docking study. The crystal structure of ACE as a molecular target was obtained from the PDB database (PDB ID: 1UZF) with captopril as a native ligand. Molecular docking analysis was performed using AutoDockZn (100 docking runs) based on the active site of Zn 2+ , the central grid was placed on Zn 2+ with a box size of 40Á × 40Á × 40Á and a center of 40.835Á × 34.382Á × 44.607Á for selective inhibitors (MCO702) with a spacing of 0.375Á. Based on the docking results demonstrated that the prediction of each polyphenol compounds from P. pellucida has the potential of active as ACE inhibitors, it was indicated that docking results of each compound has lower affinity compared to captopril (with binding affinity of −6.36 kcal/mol and the inhibition constant 21.81 μM), where the most moderate binding affinity (the most potential) was tetrahydrofuran lignin ((1R,2S,3S,5R)-3,5-bis(4-hydroxy-3,5-dimethoxyphenyl)cyclopentane-1,2-diyl)bis-(methylene) diacetate) of −8.66 kcal/mol and the highest binding affinity (the less potential) was dillapiole (6-allyl-4,5-dimethoxybenzo[d][1,3]dioxole) of −4.99 kcal/mol, although with different forms of interaction, bond, and constant inhibition. Based on the interaction of ACE binding site, 5,6,7-trimethoxy-4-(2,4,5-trimethoxyphenyl)-3,4-dihydronaphthalen-1(2H)-one showed the most similar interaction with the captopril ligand. These results are preliminary data for further research with predictions of target compound biological activity and interaction quickly, accurately, and inexpensively.

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Section: Resultsmentioning

confidence: 99%

Angiotensin-converting enzyme inhibitory activity of polyphenolic compounds from Peperomia pellucida (L) Kunth: An in silico molecular docking study

Ahmad

Azminah

Mulia³

et al. 2019

J App Pharma Sci

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“…The results showed that P. pellucida extract had total polyphenol and flavonoid content of 359.91±0.77 mg GAE/g extract and 200.28±1.23 mg QE/g extract, respectively. In the studies of Adhitia et al (2017), Ahmad et al (2018), andNg et al (2021), polyphenols and flavonoids were determined to be present in the plant. Therefore, it can be seen that P. pellucida is capable of synthesizing high content of total polyphenols and flavonoids.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and in vitro antidiabetic activities of Peperomia pellucida (L.) Kunth extract

Men¹,

Tu²,

Ánh³

et al. 2022

VIS

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Peperomia pellucida (L.) is commonly used as a herbal plant. Its effectiveness in treating inflammatory diseases, digestive disorders, and cancer in tropical and subtropical countries was introduced, especially in field of folk medicine. However, this plant species has not been studied widely in Vietnam, especially for its biological activities. This study was done to determine the antioxidant capacity of P. pellucida by using in vitro and in vivo methods, as well as its inhibitory ability to α-amylase enzyme activity. The total polyphenolic and flavonoid contents of P. pellucida extract were reported to be 359.91±0.77 mg GAE/g and 200.28±1.23 mg QE/g extract, respectively. The results showed the in vitro antioxidant activity of P. pellucida extract in four methods, including DPPH, and ABTS.+, RP and TAC, had EC50 values of 730.34 μg/mL, 84.33 μg/mL, 95.28 μg/mL, respectively, and Abs0.5 value of 114.73 μg/mL. Under H2O2-induced oxidative stress, fruit flies that were raised in the feed medium supplemented with a concentration of 1 mg/mL of P. pellucida extract showed their average survival time, 50% survival time, and 10% survival time at 1.6 times, 1.8 times, and 1.62 times, respectively, higher than those of the control treatment. The ability to inhibit the α-amylase activity in P. pellucida extract was determined with an EC50 value of 115.32±2.65 μg/mL compared with the commercial drug of 18.67±0, 01 μg/mL. The research results showed that P. pellucida is a potential species in the study of natural compounds with antioxidant and antidiabetic activities.

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“…It is used ethnobotanically for treating cough, sores, boil, fracture, conjunctivitis, asthma, hypertension, urinary infection, gastro-intestinal disorder, kidney disease. Also, its pharmacological potential has been reported as anticancer, antibacterial, anti-inflammatory, analgesic, hypotensive, antidiabetic, fibrinolytic, antisickling and antidiarrhea (Adhitia et al, 2017). Phytochemical screening revealed that Peperomia pellucida contains glycosides, F I G U R E 1 Structures of some drugs used for managing hypertension anthraquinones, terpenoids and tannins (Saputri et al, 2015).…”

Section: Peperomia Pellucidamentioning

confidence: 99%

“…Their investigation revealed that the inhibitory effect of Peperomia pellucida was more potent than captopril (the reference drug used as positive control). Adhitia et al (2017) reported the ability of ethanol extract of Peperomia pellucida to inhibit ACE, an enzyme which activity is linked to hypertension. Their investigation revealed that subjecting the extract to about 10 kGy cobalt-60 radiation did not significantly affect its ability to inhibit ACE.…”

Section: Peperomia Pellucidamentioning

confidence: 99%

A review of some common African spices with antihypertensive potential

Adeoye

Joel

Igunnu

et al. 2021

Journal of Food Biochemistry

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Hypertension is the most common non-communicable disease, with about 1.28 billion hypertensive people worldwide. It is more prevalent in men than women and more common in the elderly. Hereditary, age, obesity, lifestyle, diet, alcohol, and chronic metabolic diseases are the major risk factors of hypertension. Treating hypertension is a complex process as there are several mechanisms responsible for its pathogenesis; hence, a combination of several drugs is used for managing hypertension. Drugs used in managing hypertension are expensive and often come with associated side effects; thus, there is need for alternative means of managing this life-threatening disease. These drugs do not achieve the recommended blood pressure target in most people; more so majority of people with hypertension do not follow the treatment regimen religiously. Some Africans have been reported to become normotensive as a result of dietary consumption of spices. Several spices have been used over the years in Africa to manage hypertension. The aim of this review is to evaluate the ethnomedicinal use, bioactive phytochemical composition, bioactive compounds present, and pharmacological applications of spices commonly used in Africa for managing hypertension. Most of the plants used contained polyphenols, flavonoids, tannins, anthraquinone, flavonoids, cardiac glycosides, and saponins. Dietary supplementation of Xylopia aethiopica and other spices in diet have been proven to significantly reduced plasma angiotensin-I-converting enzyme (ACE) than simvastatin (the reference drug). Toxicological, histological, and hematological evaluation revealed that acute and chronic consumption of most of these spices are safe. Studies have also revealed that some of the spices can be used as alternative therapy alongside usual antihypertensive medications. Practical implication:The prevalent rate of hypertension is on the increase in both the developed and developing countries. People often skip medication due to their busy schedule and anti-hypertensive potential side effects; however, this is not the case with food/spices as most people consumed them daily. Deliberate, right combinations and consistent incorporation of spices with proven anti-hypertensive potential into our diet may be of great benefit in normalizing blood pressure and mitigate other complications on the heart and vital organs.Hypertension or elevated blood pressure means the persistent force of blood against the artery wall leading to increase in blood pressure. According to World Health Organisation (WHO, 2021), blood pressure on two different days with both days exceeding 140/90 mmHg is classified as hypertension. The first (higher) number is the systolic, which is the blood pressure when the heart contracts, while the second (lower number) is the diastolic, which is the blood pressure when the heart relaxes (Ondimu et al., 2019). It is usually measured using upper arm cuff; however wrist devices are used for obese persons with extreme size of the arm (Nerenberg et al., 2018). Accordi...

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Effect of Gamma Irradiation on Angiotensin Converting Enzyme Inhibition, Antioxidant Activity, Total Phenolic Compound and Total Flavonoid of Peperomia pellucida Herbs Extract

Cited by 5 publications

References 12 publications

Angiotensin-converting enzyme inhibitory activity of polyphenolic compounds from Peperomia pellucida (L) Kunth: An in silico molecular docking study

Angiotensin-converting enzyme inhibitory activity of polyphenolic compounds from Peperomia pellucida (L) Kunth: An in silico molecular docking study

Antioxidant and in vitro antidiabetic activities of Peperomia pellucida (L.) Kunth extract

A review of some common African spices with antihypertensive potential

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