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A novel benchtop approach to fabricate xurography-enabled thermally transferred (XTT) carbon nanomaterial-based electrochemical sensors is proposed. Filtered nanomaterial (NM) films were transferred from Teflon filters to polyethylene terephthalate–ethylene vinyl acetate (PET-EVA) substrates by a temperature-driven approach. Customized PET-EVA components were xurographically patterned by a cutting plotter. The smart design of PET-EVA films enabled us to selectively transfer the nanomaterial to the exposed EVA side of the substrate. Hence, the substrate played an active role in selectively controlling where nanomaterial transfer occurred allowing us to design different working electrode geometries. Counter and reference electrodes were integrated by a stencil-printing approach, and the whole device was assembled by thermal lamination. To prove the versatility of the technology, XTT materials were exclusively made of carbon black (XTT-CB), multiwalled carbon nanotubes (XTT-MWCNTs), and single-walled carbon nanotubes (XTT-SWCNTs). Their electrochemical behavior was carefully studied and was found to be highly dependent on the amount and type of NM employed. XTT-SWCNTs were demonstrated to be the best-performing sensors, and they were employed for the determination of l-tyrosine (l-Tyr) in human plasma from tyrosinemia-diagnosed patients. High analytical performance toward l-Tyr (linear range of 0.5–100 μM, LOD = 0.1 μM), interelectrode precision (RSD i p,a = 3%, n = 10; RSD calibration slope = 4%, n = 3), and accurate l-Tyr quantification in plasma samples with low relative errors (≤7%) compared to the clinical declared values were obtained. The proposed benchtop approach is cost-effective and straightforward, does not require sophisticated facilities, and can be potentially employed to develop pure or hybrid nanomaterial-based electrodes.

Surface-enhanced Raman scattering (SERS) spectroscopy stands out due to its sensitivity, selectivity, and multiplex ability. The development of ready-to-use, simple, and low-cost SERS substrates is one of the main challenges of the field. In this paper, the intrinsic reproducibility of microfluidics technology was used for the fabrication of self-assembled nanoparticle structures over a paper film. The paper SERS substrates were fabricated by assembling anisotropic particles, gold nanostars (GNSs), and nanorods (NRs) onto paper to offer an extra enhancement to reach ultra-sensitive detection limits. A polydimethylsiloxane PDMS-paper hybrid device was used to control the drying kinetics of the nanoparticles over the paper substrate. This method allowed a high reproducibility and homogeneity of the fabrication of SERS substrates that reach limits of detection down to the picomolar range. This simple and low-cost fabrication of a paper-based sensing device was tested for the discrimination of different cell lineages.

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Organ on-a-chip (OoC) is a promising technology that aims to recapitulate human body pathophysiology in a more precise way to advance in drug development and complex disease understanding. However, the...