Oxidative stress on-chip: Prussian blue-based electrode array for in situ detection of H2O2 from cell populations

Rojas, Daniel; Hernández-Rodríguez, Juan F.; Pelle, Flavio Della; Carlo, Michele Del; Compagnone, Darío; Escarpa, Alberto

doi:10.1016/j.bios.2020.112669

Cited by 28 publications

(18 citation statements)

References 25 publications

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“…S10 in the ESM). The concentration of H 2 O 2 was calculated to be 8.04 μM and the corresponding mass of H 2 O 2 released from a single cell was about 2.68 fmol, which is in the similar order of magnitude to some other reported methods (Table S2 in the ESM) [42,43].…”

Section: Colorimetric Detection For H 2 Osupporting

confidence: 79%

Versatile Ti3C2Tx MXene for free-radical scavenging

Liu

Lü

et al. 2021

Nano Res.

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MXene, as an emerging two-dimensional (2D) material with ultrathin structure and fascinating physiochemical properties, has been widely explored in broad applications. Versatile functions of MXenes are continuously explored. This work presents distinctive feature of MXene-Ti 3 C 2 T x nanosheets for free-radical (FRs) scavenging that never reported before. We demonstrated the mechanism and equation in regard to the reaction between Ti 3 C 2 T x and H 2 O 2 , which was applied to design colorimetric H 2 O 2 strip assay with good performance. The good FRs scavenging capability of Ti 3 C 2 T x , including a series of reactive oxygen species (ROS) and reactive nitrogen species (RNS), was systemically confirmed. The antioxidation capability of Ti 3 C 2 T x for protecting cells from oxidative damage was demonstrated using the oxidative damage model of alpha mouse liver 12 (AML-12) cells. This original work provides huge opportunities for MXenes in FR-related biomedical applications. Electronic Supplementary Material Supplementary material (further details of the experimental procedures, investigation of the reaction between Ti 3 C 2 T x and other oxidants, the characterization of endocytosis of cells for Ti 3 C 2 T x , and the comparison of different antioxidants for scavenging free radicals) is available in the online version of this article at 10.1007/s12274-021-3751-y.

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Section: Colorimetric Detection For H 2 Osupporting

confidence: 79%

Versatile Ti3C2Tx MXene for free-radical scavenging

Liu

Lü

et al. 2021

Nano Res.

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“…From the intensities of the peaks vs scan rates (plot reported in Fig. S4), for both anodic and cathodic peaks, a linear trend is evident, suggesting a diffusionless process, with an electron transfer confined to the surface of the electrode [37].…”

Section: Electrochemical Features Of the Redox-active Graphenementioning

confidence: 98%

(+)-Catechin-assisted graphene production by sonochemical exfoliation in water. A new redox-active nanomaterial for electromediated sensing

et al. 2021

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A new green and effective sonochemical liquid-phase exfoliation (LPE) is proposed wherein a flavonoid compound, catechin (CT), promotes the formation of conductive, redox-active, water-phase stable graphene nanoflakes (GF). To maximize the GF-CT redox activity, the CT concentration and sonication time have been studied, and the best performing nanomaterialfraction selected. Physicochemical and electrochemical methods have been employed to characterize the morphological, structural, and electrochemical features of the GF-CT nanoflakes. The obtained GF intercalated with CT exhibits fully reversible electrochemistry (ΔE p = 28 mV, ipa/ipc = ⁓1) because of the catecholic adducts. GF-CT-integrated electrochemistry was generated directly during LPE of graphite, with no need of graphene oxide production, nor activation steps, electropolymerization, or ex-post functionalization. The GF-CT electro-mediator ability has been proven towards hydrazine (HY) and β-nicotinamide adenine dinucleotide (NADH) by simply drop-casting the redox-material onto screen-printed electrodes. GF-CT-based electrodes by using amperometry exhibited high sensitivity and extended linear ranges (HY: LOD = 0.1 µM, L.R. 0.5-150 µM; NADH: LOD = 0.6 µM, L.R. 2.5-200 µM) at low overpotential (+ 0.15 V) with no electrode fouling. The GF-CT electrodes are performing significantly better than commercial graphite electrodes and graphene nanoflakes exfoliated with a conventional surfactant, such as sodium cholate. Recoveries of 94-107% with RSD ≤ 8% (n = 3) for determination of HY and NADH in environmental and biological samples were achieved, proving the material functionality also in challenging analytical media. The presented GF-CT is a new functional redox-active material obtainable with a single-pot sustainable strategy, exhibiting standout properties particularly prone to (bio)sensors and cutting-edge device development.

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“…Although ROS and RNS are short-lived species that are mainly located within subcellular spaces, the most stable species can be also detected extracellularly. Rojas et al developed a low-cost electrode array based on Prussian Blue to detect H 2 O 2 produced by HeLa in real time upon stimulation with fMLP . Also, Zhang et al reported a conductive polymer scaffold (PEDOT/Pt NPs) to grow HeLa and MCF-7 cells and detect in situ hydrogen peroxide generated as a response to treatment with a promising anticancer drug .…”

Section: Analysis Of Cell Cultures and Microphysiological Models: An ...mentioning

confidence: 99%

“…Rojas et al developed a low-cost electrode array based on Prussian Blue to detect H 2 O 2 produced by HeLa in real time upon stimulation with fMLP. 160 Also, Zhang et al reported a conductive polymer scaffold (PEDOT/Pt NPs) to grow HeLa and MCF-7 cells and detect in situ hydrogen peroxide generated as a response to treatment with a promising anticancer drug. 161 Other reactive species have been reported.…”

Section: ■ Analysis Of Cell Cultures and Microphysiological Models: A...mentioning

confidence: 99%

Electrochemical Sensing Directions for Next-Generation Healthcare: Trends, Challenges, and Frontiers

2020

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Oxidative stress on-chip: Prussian blue-based electrode array for in situ detection of H2O2 from cell populations

Cited by 28 publications

References 25 publications

Versatile Ti3C2Tx MXene for free-radical scavenging

Versatile Ti3C2Tx MXene for free-radical scavenging

(+)-Catechin-assisted graphene production by sonochemical exfoliation in water. A new redox-active nanomaterial for electromediated sensing

Electrochemical Sensing Directions for Next-Generation Healthcare: Trends, Challenges, and Frontiers

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