(2013). Genetic structure of Tetraclinis articulata, an endangered conifer of the western Mediterranean basin. Silva Fennica vol. 45 no. 5 article id 1073. 14 p.
Highlights• The employment of ISSR molecular markers has shown moderate genetic diversity and high genetic differentiation in Tetraclinis articulata.• Genetic structure of populations seems to be influenced by the anthropogenic use of this species since historical times, or alternatively, by the complex palaeogeographic history of the Mediterranean basin.• Results could be used to propose management policies for conservation of populations.
AbstractTetraclinis articulata (Vahl) Masters is a tree distributed throughout the western Mediterranean basin. It is included in the IUCN (International Union for Conservation of Nature) red list, and protected by law in several of the countries where it grows. In this study we examined the genetic diversity and genetic structure of 14 populations of T. articulata in its whole geographic range using ISSR (inter simple sequence repeat) markers. T. articulata showed moderate genetic diversity at intrapopulation level and high genetic differentiation. The distribution of genetic diversity among populations did not exhibit a linear pattern related to geographic distances, since all analyses (principal coordinate analysis, Unweighted pair group method with arithmetic mean dendrogram and Bayesian structure analysis) revealed that spanish population grouped with Malta and Tunisia populations. Although it is possible that T. articulata earlier was natural in Southeast Spain, results suggest that the current population has been reintroduced into the Iberian Peninsula in historical times, due to its utility in mining and building. In addition, results could be used to propose management guidelines for the conservation of T. articulata.
& Key message The genetic structure of Juniperus phoenicea in the Mediterranean Basin is inferred using amplified fragment length polymorphism markers (AFLP) markers. As other Mediterranean conifers, J. phoenicea populations show moderate levels of genetic diversity and interpopulational differentiation. The pattern of distribution of genetic diversity seems highly influenced by the climatic fluctuations which occurred in the Pleistocene. & Context It has been stated that the genetic structure of Mediterranean conifers is mediated by the historical climatic changes and the geological rearrangements which occurred in the Mediterranean Basin. J. phoenicea provides an excellent example to test how its genetic structure is influenced by these events. & Aims In this work, we study the amount and distribution of genetic diversity of J. phoenicea complex, in order to evaluate its taxonomic status and to reveal underlying phylogeographic patterns. & Methods The molecular diversity was analyzed for 805 individuals from 46 populations throughout its distribution range using AFLP markers. Principal coordinate analysis, analysis of molecular variance (AMOVA), and Bayesian-based analysis were applied to examine the population structure.
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