Polarographic determination of thiamine and its monophosphate and pyrophosphate esters

Vergara, T.; Marín, Dolores Pérez; Vera, Juan B.

doi:10.1016/s0003-2670(01)84378-6

Cited by 18 publications

(10 citation statements)

References 5 publications

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“…Mercury electrodes have been widely used in the voltammetric determination of vitamin B 1 in aqueous solutions [15][16][17][18]. Their higher overpotential for hydrogen discharge relative to other electrode materials such as platinum, gold, and carbon, makes them the electrode material of choice for the electrochemical study of many important biological compounds [22].…”

Section: Practical Considerations Of the Electrode Materialsmentioning

confidence: 99%

“…Their higher overpotential for hydrogen discharge relative to other electrode materials such as platinum, gold, and carbon, makes them the electrode material of choice for the electrochemical study of many important biological compounds [22]. Mercury, however, can bind to thiamin [15] and influence its redox behavior. In the report by Rao and co-workers [16] on the electroreduction of thiamin in neutral and acidic aqueous solutions, multiple adsorption peaks attributed to pre-and post-adsorption of thiamin and to the hydrogen discharge were observed on the mercury electrode.…”

Section: Practical Considerations Of the Electrode Materialsmentioning

confidence: 99%

“…1) [10][11][12]. Since the discovery by Breslow nearly five decades ago, that the ionization at the C2 position is key to the reactivity of thiamin [13], most electrochemical studies of thiamin conducted to date have attributed the redox behavior observed to the thiazolium ring [14][15][16][17][18]. With the recent knowledge that the 4 0 -aminopyrimidine participates in catalysis (when protonated at the N1 0 position, A 2þ ) [11,12,19,20], this report is the first to illustrate the unusual electrochemical properties of thiamin and its phosphate esters as activated by the protonation at the N1 0 position in the pyrimidine ring.…”

Section: Introductionmentioning

confidence: 99%

“…While the electrochemistry of thiamin and its phosphate esters in alkaline solutions on modified and on mercury electrodes has been known for a long time [14,15,17,18], there has been very little effort devoted to the study of the electrochemical behavior of these compounds in acidic media [16], the knowledge of which may shed more light on the way thiamin dependent enzymes catalyze reactions. To our knowledge, the polarographic study of thiamin in acidic media conducted by Rao and co-workers two decades ago [16] provides the only comprehensive report on the reduction of thiamin in acidic solutions that has come forth.…”

Section: Introductionmentioning

confidence: 99%

“…To our knowledge, the polarographic study of thiamin in acidic media conducted by Rao and co-workers two decades ago [16] provides the only comprehensive report on the reduction of thiamin in acidic solutions that has come forth. Also, because of the tendency of mercury electrodes to bind to electroactive species and participate in redox reactions as catalysts [15,21], more definitive redox information about thiamin obtained on inert or less reactive electrode materials at a pH resembling that of biological environments remains unavailable.…”

Section: Introductionmentioning

confidence: 99%

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Activation of the electrochemical properties of thiamin and its phosphate esters in acidic solutions

Sutton

Shabangi

2004

Journal of Electroanalytical Chemistry

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Section: Practical Considerations Of the Electrode Materialsmentioning

confidence: 99%

Section: Practical Considerations Of the Electrode Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Activation of the electrochemical properties of thiamin and its phosphate esters in acidic solutions

Sutton

Shabangi

2004

Journal of Electroanalytical Chemistry

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Electroanalysis and Biosensors in Clinical Chemistry

Killard

Sequeira

Diamond

et al. 2000

Encyclopedia of Analytical Chemistry

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Electroanalysis involves the measurement or determination of a chemical or biochemical species, using a wide range of electrochemical methods. The species being measured can either bring about a direct electrochemical response that can be detected and measured, or can be induced to bring about such a response, resulting in a process that can be detected electrochemically. In both cases an interface is required between the chemical or biochemical species being measured, and the electrochemical system performing the measurement. This interface is referred to as an “electrode”. It is the transfer of electrons or ions between the electrode and the environment being sampled which is of concern in electroanalysis. The application of electrochemistry in clinical analysis is concerned with the measurement of chemical and biochemical species present in biological fluids (primarily blood but also urine and other body fluids and tissues). It is applied in several areas of critical care where its rapidity, sensitivity, precision, and accuracy at biologically relevant concentrations make it an ideal choice over other, typically slower, biochemical techniques. Indeed, it is the speed of electrochemical analysis that principally differentiates it from other methods. It is not universally applicable to clinical chemistry, however, and has only been applied in situations where such analyses are relevant. This has principally been in the analysis of blood gases and pH and blood electrolytes. Electrochemical methods are, however, gradually being extended to the analysis of small biochemical components such as sugars, amino acids and other metabolites, with the advent of enzyme‐based “biosensors”. There is also little doubt that the application of electrochemistry in clinical analysis will be further exploited by the use of such biosensor devices based on other forms of biorecognition, such as antibodies, cell ligands, membranes, tissues and whole cells. These biosensor devices are also amenable to other forms of detection such as acoustic wave and optical measurements. Together, these techniques offer a wide range of advantages over traditional analytical methodologies, and will undoubtedly have a rapidly increasing impact on many types of clinical analysis. This article focuses on the use of electrochemical techniques and electrochemical biosensors and their application in clinical chemistry.

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Polarographische Verfahren [1–8]

Henze

2001

Polarographie Und Voltammetrie

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Polarographic determination of thiamine and its monophosphate and pyrophosphate esters

Cited by 18 publications

References 5 publications

Activation of the electrochemical properties of thiamin and its phosphate esters in acidic solutions

Activation of the electrochemical properties of thiamin and its phosphate esters in acidic solutions

Electroanalysis and Biosensors in Clinical Chemistry

Polarographische Verfahren [1–8]

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