The objectives of this experiment were to evaluate the effect of feeding a culture of Saccharomyces cerevisiae on rumen metabolism and digestibility when cows are fed diets varying in starch content. Four lactating Holstein cows were assigned to a 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments. Treatments were low starch (LS; 23% of diet DM) and no yeast culture (YC; LS-control), LS and 15 g of YC/d (LS-YC), high starch (HS; 29% of diet DM) and no YC (HS-control), and HS and 15 g of YC/d (HS-YC). Periods lasted 28 d, with the last 9 d for data collection. Days 20 to 24 were used to determine production, nutrient flow, and digestibility. On d 25, 3 kg of corn grain DM was placed in the rumen 1 h before the morning feeding, and yields of milk and milk components were measured after the challenge. Blood was sampled -1, 3, 7, and 11 h relative to the morning feeding on d 24 and 25. Rumen pH was measured continuously on d 24 and 25. Rumen papillae were collected on d 24 and 28 to quantify mRNA expression of select genes. Supplementing YC increased yields of milk (26.3 vs. 29.6 kg/d), energy-corrected milk (ECM; 26.5 vs. 30.3 kg/d), fat (0.94 vs. 1.08 kg/d), true protein (0.84 vs. 0.96 kg/d), and ECM/dry matter intake (1.15 vs. 1.30) compared with the control but did not affect dry matter intake (22.6 vs. 22.9 kg/d). Cows fed HS had increased milk true protein percentage (3.18 vs. 3.31%) and yield (0.87 vs. 0.94 kg/d) compared with cows fed LS. Feeding HS-YC increased the proportion of dietary N incorporated into milk true protein from 24.9% in the other 3 treatments to 29.6%. Feeding HS increased the concentration of propionate (21.7 vs. 32.3 mM) and reduced that of NH-N (8.3 vs. 6.7 mg/dL) in rumen fluid compared with the control, and combining HS with YC in HS-YC tended to increase microbial N synthesis compared with LS-YC (275 vs. 322 g/d). Supplementing YC to cows fed HS reduced plasma haptoglobin and rumen lactate concentrations, increased mean rumen pH, reduced the time with pH <6.0, and prevented the decrease in rumen neutral detergent fiber digestion caused by HS. Cows fed HS had less total-tract digestion of organic matter (73.9 vs. 72.4%) because of reduced acid detergent fiber (57.6 vs. 51.7%) and neutral detergent fiber (60.9 vs. 56.7%) digestibility. Production performance after the challenge was similar to that before the challenge, and YC improved yield of ECM. After the challenge, supplementing YC tended to reduce rumen lactate concentration compared with the control and reduced haptoglobin in cows fed HS. Feeding HS but not YC increased expression in rumen papillae of genes for receptors (FFAR2 and FFAR3) and transporter (SLC16A3) of short-chain fatty acids but did not affect genes involved in transport of Na/H or water or in inflammatory response. Supplementing YC to dairy cows improved lactation performance in diets containing low or high starch, and mechanisms might be partially attributed to improvements in rumen pH, digestion of fiber, microbial N synthesis, and reduct...

The objectives were to evaluate the effects of a culture of Saccharomyces cerevisiae (YC) on lactation performance of cows fed diets differing in starch content. Fifty-six Holstein cows at 42 d postpartum were blocked by parity and milk production and randomly assigned to 1 of 4 treatments, low starch (23% diet DM) and no YC (LS-control), low starch and 15 g/d of YC (LS-YC), high starch (29% diet DM) and no YC (HS-control), and high starch and 15 g/d of YC (HS-YC). The experiment lasted 14 wk. Blood was sampled twice weekly during the first 5 wk in the experiment. Feeding behavior was evaluated in 2 consecutive days when cows were 33 d in the experiment. On d 92 in the experiment, cows were challenged with 3 kg of corn grain DM immediately before the morning feeding. Blood was sampled in the first 12 h after the challenge. Rumen fluid was collected 5 h after the challenge, and pH, ammonia N, short-chain fatty acids, and lactate concentrations were quantified. Lactation performance was measured daily before and after the challenge. Supplementation with YC increased yields of 3.5% fat-corrected milk and energy-corrected milk by 2.2 and 2.0 kg/d, and the increments were observed in both low- and high-starch diets. Feeding HS tended to decrease milk fat content (LS = 3.88 vs. HS = 3.73%), but increased concentration (LS = 2.87 vs. HS = 3.00%) and yield (LS = 1.11 vs. HS = 1.20 kg/d) of milk true protein. Feeding YC increased yields of fat and true protein in milk by 100 and 60 g/d. Energy balance, body weight, and feed efficiency did not differ with treatments. Feeding HS reduced eating time (LS = 177 vs. HS = 159 min/12 h) and intermeal interval (LS = 103 vs. HS = 82 min), but tended to increase eating rate (LS = 139 vs. HS = 150 g/min). Interactions were detected between level of starch and YC for ruminating time, meal duration, and meal size because within LS, feeding YC increased ruminating time 23 min/12 h, but reduced meal duration 6 min/meal and meal size 0.7 kg/meal. Concentrations of glucose in plasma increased (LS = 62.1 vs. HS = 63.8 mg/dL), whereas those of urea N decreased (LS = 10.1 vs. HS = 9.4 mg/dL) with feeding HS compared with LS in the first 5 wk in the experiment, and the same responses were observed after the challenge with corn grain. After the challenge, rumen pH was less and short-chain fatty acid concentrations were greater in cows fed HS compared with those fed LS; however, supplementing YC to high-starch diets increased rumen pH (HS-control = 5.72 vs. HS-YC = 6.12) and reduced concentrations of lactate in rumen fluid (HS-control = 7.72 vs. HS-YC = 1.33 mM) and haptoglobin in plasma 28%. Feeding YC improved lactation performance irrespective of the level of dietary starch and reduced the risk of subacute rumen acidosis induced by a grain challenge when cows were fed a high-starch ration.

RESUMO -O experimento foi realizado com o objetivo de avaliar a substituição da silagem de milho por cana-de-açúcar em dietas para vacas leiteiras. Foram avaliados quatro níveis de substituição (0; 33,3; 66,6 e 100%) de silagem de milho por cana-de-açúcar para o estudo do consumo e da digestibilidade aparente dos nutrientes e a determinação do pH e da concentração de compostos nitrogenados amoniacais (N-NH 3 ) do líquido ruminal e da taxa de passagem ruminal da digesta. Doze vacas da raça Holandesa, puras e mestiças, com potencial para produção de 5.000 a 7.000 kg de leite por lactação, foram distribuídas em três quadrados latinos 4 x 4 balanceados, conforme o período de lactação. Os animais foram mantidos em baias individuais, onde receberam as dietas (ad libitum), duas vezes ao dia, com relação volumoso:concentrado 60:40, durante 84 dias experimentais. O aumento do nível de substituição de silagem de milho por cana-de-açúcar promoveu redução linear do consumo de todos os nutrientes, exceto lignina e carboidratos não-fibrosos. A substituição não afetou as digestibilidades da matéria seca, da matéria orgânica e da proteína bruta. A digestibilidade da fibra em detergente neutro apresentou redução acentuada, enquanto a dos carboidratos não-fibrosos aumentou. O pH ruminal não foi influenciado pelas dietas, apresentando comportamento quadrático conforme o tempo após alimentação. As concentrações de amônia ruminal apresentaram comportamento quadrático de acordo com as dietas e o tempo de alimentação. A taxa de passagem ruminal da digesta diminuiu e o tempo médio de retenção total aumentou com a substituição, o que pode explicar a redução no consumo. A silagem de milho pode ser substituída em até 33% pela cana-de-açúcar para vacas com produções médias diárias de 24 kg de leite.Palavras-chave: consumo, digestibilidade, pH e amônia ruminais Effects of replacing corn silage with sugarcane on production and ruminal metabolism of lactating dairy cowsABSTRACT -This trial was conducted to evaluate the effects of replacing corn silage with sugarcane on intake, apparent digestibility of nutrients, ruminal pH and ammonia nitrogen, and ruminal passage rate of digesta in lactating dairy cows.Proportion sugarcane increased as follows: 0, 33.3, 66.6 and 100% of the forage portion of the diet. Twelve Holstein and crossbred Holstein/Zebu cows with potential yield varying from 5,000 to 7,000 kg of milk per lactation were randomly assigned to three 4x4 Latin squares. Cows were maintained in individual stalls and were fed ad libitum twice a day a diet containing a forage:concentrate ratio of 60:40 during the 84-days experimental period. Replacing corn silage with sugarcane linearly reduced the intakes of all nutrients with the exception of lignin and nonfiber carbohydrates. No significant effects on apparent digestibilities of dry matter, organic matter and crude protein were observed by replacing corn silage with sugarcane in this trial. However, neutral detergent fiber digestibility decreased and that of nonfiber carbohydrates increas...

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Objetivou-se estimar a composição do ganho e as exigências de energia e proteína para ganho de peso em bovinos Nelore puros e mestiços. Utilizaram-se 60 bovinos machos (Nelore, F1 Nelore x Angus, F1 Nelore x Pardo-Suíço e F1 Nelore x Simental) não-castrados, com 10 a 11 meses de idade e peso médio inicial de 286, 309, 333 e 310 kg, respectivamente. O delineamento utilizado foi o inteiramente casualizado, com quatro grupos genéticos, submetidos a quatro níveis de concentrado na ração (30, 40, 60 e 70%). Três animais de cada grupo genético foram abatidos ao início do experimento e serviram como referência na determinação da composição corporal inicial. As exigências líquidas de proteína e energia para ganho de 1 kg de peso corporal vazio (PCV) foram estimadas pela equação Y' = a. b. X (b-1), em que a e b são os coeficientes das equações de regressão dos conteúdos de energia e proteína e X, o PCV dos animais. O teste de identidade de modelos não-lineares indicou não haver diferenças entre grupos genéticos para as exigências de energia e proteína para ganho de peso. Verificou-se decréscimo de 10,6% nas exigências de proteína e elevação de 37,8% nas exigências de energia para ganho de peso entre 250 e 550 kg, o que está relacionado à elevação do teor de gordura e à redução no teor de proteína com o acréscimo no PCV. As exigências líquidas de proteína e energia para ganho de peso foram estimadas em 143,5 g e 4,7 Mcal para o peso vivo de 450 kg.

51,1; 17,9; 190,5; 280,4 e -12,0 para P120; 128,6; 47,2; 695,7; 932,4 e -25,9 para P205; 435,9; 32,2; 2934,0; 3604,9 e -27,4 para P365; 607,9; 177,7; 5637,7; 6602,5 e -117,6 para P550. Os valores de herdabilidades direta encontram-se na faixa de 0,09 a 0,18; herdabilidade materna variaram de 0,01 a 0,06, correlação direta materna entre -0,23 a -0,36 e o efeito de ambiente permanente igual a 0,05 a 0,12. As estimativas das tendências genéticas, direta materna, e ambientais no período de 1975 a 2001 foram 10,4; -1,6 e 18,4 kg para P120; 16,6; -2,8 e 23,0 kg P205; 21,7; -1,3 e 23,6 kg para P365; 25,5; -3,6 e -8,1 kg para P550, respectivamente. As tendências genéticas diretas foram positivas e as maternas pequenas e negativas. O desempenho materno tem influencia importante sobre os pesos aos 120 e 205 dias de idade e esse baixo valor obtido sugere que se deve considerar para este efeito nos programas de melhoramento, mesmo se tendo correlação direta materna adversa. SUMMARY

The objective of this study was to evaluate the carcass and meat traits, and the non-carcass components of crossbred Dorper lambs fed diets with increasing levels of urea (0.0, 0.5, 1.0, and 1.5% of dry matter – DM). The experimental design was completely randomized with four treatments (urea inclusion levels) and six replicates per treatment. Lambs were fed ad libitum for 56 days and slaughtered at 37.9 ± 5.1 kg of body weight (BW). The weight and yield of carcass before and after cooling were not influenced by urea levels, with average values of 16.9 kg and 44.6% for cold carcass weight and yield. Urea levels did not affect the morphometric measurements, the fat deposition on the carcass, the weight of carcass cuts and the weight of non-carcass components. There was a quadratic effect of urea levels on the loin yield, which may achieve maximum value of 11.31% with the inclusion of 0.84% DM urea in the feed. The pH and the color coordinates L* (brightness), a* (red intensity) and C* (saturation) of the meat also showed quadratic response to the urea levels, where in the minimum value of 5.53 for pH, maximum value of 48.67 for L* and minimum values of 14.04 and 16.21 for a* and C* may be obtained by including 0.53 to 0.70% DM urea in the ration. The inclusion of 0.84% DM urea in the ration is recommended to obtain maximum yield of loin and meat with attractive characteristics to the consumer, which is characterized by high red intensity and brightness. If consumers have preference for lamb meat with a more intense red color, the inclusion of 1.5% DM urea should be considered in the ration formulation.

Objetivou-se estimar parâmetros, correlações e tendências genéticas para os pesos de animais da raça Nelore criados na região da Mata e no Agreste Nordestino aos 205 (P205), 365 (P365) e 550 (P550) dias de idade. Para realização das análises genéticas, utilizou-se da metodologia de modelos mistos. O modelo estatístico continha os efeitos aleatórios aditivos diretos, aditivos maternos, ambiente permanente e o erro; efeitos fixos de grupo contemporâneo - fazenda, sexo, estação (água e seca), mês e ano de nascimento do bezerro e a covariável idade da vaca ao parto. As tendências genéticas foram estimadas pela regressão dos valores genéticos sobre o ano de nascimento dos animais. As estimativas da herdabilidade direta foram 0,13 ± 0,03 para P205; 0,03 ± 0,02 para P365 e 0,12 ± 0,04 para P550. As correlações variaram de 0,55 a 0,85. Os parâmetros genéticos permitem ganhos por meio da seleção e, de acordo com as correlações, a seleção para peso a determinada idade possibilitará ganhos as idades posteriores, podendo assim realizar-se seleções em idades mais jovens, apesar dos ganhos serem lentos devido aos baixos coeficientes de herdabilidade.